公开(公告)号：US20060189890A1

公开(公告)日：2006-08-24

申请号：US10723990

申请日：2003-11-25

Applicant: Andrew Gooley ,  Robert Ramsden ,  Kurt Weber

Inventor： Andrew Gooley ,  Robert Ramsden ,  Kurt Weber

IPC: A61B10/00

CPC classification number: G01N35/1016 ,  B01J2219/00691 ,  G01N35/0099 ,  G01N35/1065 ,  G01N2001/2873 ,  G01N2035/1044

Abstract: A device for dispensing liquids includes one or more liquid outlet means carried on a movable head. The device forms part of an automated excision apparatus for cutting biomolecule spots from an array of such spots carried in a gel. The head is mounted on an automated motion control system to allow for movement of the head in X, Y, Z directions under the control of a control means. A displacement means for causing the dispensing of liquid from the one or more liquid outlet means of the device is also carried by the moveable head carrying the liquid delivery outlets allowing the length of the tubing between the outlet and the aspiration device, typically a syringe, to be much shorter. This means that swelling and contraction of the tube is minimized during liquid handling operations which means more accurate delivery of specified volumes through the outlet. The syringes and the outlets are mounted on opposite sides of an axis of movement of the apparatus, keeping the machine compact. Typically the axis will be a horizontal X or vertical Y axis defined by a beam or the like which supports the movable head.

Abstract translation: 用于分配液体的装置包括承载在可移动头上的一个或多个液体出口装置。 该装置构成用于从凝胶中携带的这种斑点的阵列切割生物分子斑点的自动切除装置的一部分。 头部安装在自动运动控制系统上，以允许头部在X，Y，Z方向上在控制装置的控制下移动。 用于引起来自装置的一个或多个液体出口装置的液体分配的位移装置也由携带液体输送出口的可移动头承载，允许出口和抽吸装置（通常为注射器）之间的管道的长度， 要短得多 这意味着在液体处理操作期间管的膨胀和收缩最小化，这意味着通过出口更精确地输送指定的体积。 注射器和出口安装在装置的运动轴线的相对侧上，保持机器紧凑。 通常，轴将是由支撑可移动头的梁等限定的水平X或垂直Y轴。

公开(公告)号：US20070008387A1

公开(公告)日：2007-01-11

申请号：US10550813

申请日：2004-03-29

Applicant: Femia Hopwood ,  Andrew Gooley

Inventor： Femia Hopwood ,  Andrew Gooley

IPC: B41J2/045

CPC classification number: B41J2/17523 ,  B41J2/17563 ,  G01N35/1002 ,  G01N2035/00158 ,  G01N2035/00237

Abstract: A piezoelectric dispensing apparatus (10) includes a piezoelectric dispensing device (20) including a fluid reservoir (20a) and a piezoelectric dispensing tube (70). The apparatus includes a seat for receiving the device and a plunger which is shaped and configured to abut with and seal the top of the reservoir and which defines a through bore to permit the application of vacuum and or pressure to the reservoir through the bore. The plunger may be moved up and down towards and away from the seat. The fluid reservoir defines an open top and an outlet at the base of the reservoir closed by a filter. The base of the reservoir define a foot. A secondary filter assembly is attachable to the base of the reservoir. The secondary filter assembly defines a bore which is in fluid communication with the reservoir when the secondary filter assembly is attached thereto, The secondary filter assembly also defines means for removably attaching the piezoelectric dispensing tube in fluid communication with the bore. The device includes a ducks bum valve or septum for closing the outlet of the reservoir until the reservoir is attached to the removable secondary filter assembly.

Abstract translation: 压电分配装置（10）包括压电分配装置（20），其包括流体储存器（20a）和压电分配管（70）。 该装置包括用于容纳该装置的座和一个柱塞，该柱塞的形状和构造与其邻接并密封储存器的顶部并且限定通孔以允许通过该孔施加真空和/或对储存器施加压力。 柱塞可以朝向和远离座椅上下移动。 流体储存器限定开放的顶部和在储存器底部的由过滤器封闭的出口。 水库的底部定义一个脚。 二次过滤器组件可附接到储存器的底部。 次级过滤器组件限定了当辅助过滤器组件附接到其上时与储存器流体连通的孔。次级过滤器组件还限定用于可拆卸地将压电分配管与孔流体连通的装置。 该装置包括用于关闭储存器的出口的鸭形凸起阀或隔膜，直到储存器附接到可移除的次级过滤器组件。

公开(公告)号：US20050124011A1

公开(公告)日：2005-06-09

申请号：US10896868

申请日：2004-07-23

Applicant: Carol Morris ,  Mark Willcox ,  Shirley Bolis ,  Bradley Walsh ,  Ben Herbert ,  Mark Molloy ,  Andrew Gooley ,  Keith Williams

Inventor： Carol Morris ,  Mark Willcox ,  Shirley Bolis ,  Bradley Walsh ,  Ben Herbert ,  Mark Molloy ,  Andrew Gooley ,  Keith Williams

IPC: C07K14/47 ,  G01N33/574 ,  G01N33/53

CPC classification number: C07K14/47 ,  G01N33/57415 ,  G01N33/57434 ,  G01N33/57488

Abstract: A method of screening or detecting non-ocular disease in an animal, the method comprising the steps of: (a) obtaining a tear sample from the animal; (b) separating biomolecules present in the tear sample; and (c) detecting for the presence or absence of one or more biomolecules such that the presence or absence of the one or more biomolecules being an indicator or marker of a disease state in the animal.

Abstract translation: 一种在动物中筛选或检测非眼部疾病的方法，所述方法包括以下步骤：（a）从动物获得泪液样品; （b）分离存在于泪液样品中的生物分子; 和（c）检测一种或多种生物分子的存在或不存在，使得一种或多种生物分子的存在或不存在是动物中疾病状态的指示剂或标志物。

公开(公告)号：US20050019942A1

公开(公告)日：2005-01-27

申请号：US10865678

申请日：2004-06-10

Applicant: Andrew Sloane ,  Malcolm Pluskal ,  Andrew Gooley ,  Janice Joss ,  Russell Ludowyke ,  Michael Hsu

Inventor： Andrew Sloane ,  Malcolm Pluskal ,  Andrew Gooley ,  Janice Joss ,  Russell Ludowyke ,  Michael Hsu

IPC: B01L3/00 ,  G01N27/447 ,  G01N35/00 ,  G01N35/02 ,  G01N21/00 ,  G01N1/00

CPC classification number: B01L3/5085 ,  G01N27/44717 ,  G01N27/44726 ,  G01N35/00029 ,  G01N35/028 ,  Y10T436/25

Abstract: Separation of macromolecules by one-dimensional or two-dimensional methods, such as gel electrophoresis, produces an array of macromolecules, which can be transferred to a support, thereby producing the same array as on the gel. In the case of one-dimensional gel electrophoresis, because of the regular spacing of the gel lanes and the predictable direction of migration of the macromolecules, the positions of the macromolecule spots or bands in the array can be predicted to be at least within the area of the support corresponding to the lanes of the gel. Where the molecular weight of a macromolecule of interest is known, molecular weight markers can be used to determine where the macromolecule band is on the support, even if the macromolecule is not stained in the gel or on the support. Assays that reveal characteristics of the macromolecule can be carried out by spotting reagents onto the support in a series of microspots of small volume in a line which intersects the macromolecule band, and which corresponds to the line of the direction of migration of the macromolecules on the gel. Appropriate detection methods can be applied, depending on the reagent, to see the results. The steps for locating the bands of macromolecules, applying reagents, and detecting the effect of the reagent on the macromolecule can be automated in an appropriate instrument.

Abstract translation: 通过一维或二维方法（例如凝胶电泳）分离大分子产生大分子阵列，其可以转移到载体上，从而产生与凝胶上相同的阵列。 在一维凝胶电泳的情况下，由于凝胶泳道的规则间隔和大分子的可预测的迁移方向，阵列中的大分子斑点或条带的位置可以预测至少在该区域内 的对应于凝胶泳道的支撑物。 当感兴趣的大分子的分子量是已知的时，分子量标记可用于确定大分子带在载体上的位置，即使大分子没有在凝胶或载体上染色。 可以通过将试剂点在与大分子带相交的一行中的小体积的一系列小体积的载体上，将大分子的迁移方向对应于大分子的迁移方向 凝胶。 可以根据试剂适用检测方法来查看结果。 用于定位大分子带，应用试剂和检测试剂对大分子的影响的步骤可以在合适的仪器中自动化。

公开(公告)号：US20080035483A1

公开(公告)日：2008-02-14

申请号：US11711808

申请日：2007-02-28

Applicant: Ben Herbert ,  Andrew Gooley ,  Keith Williams

Inventor： Ben Herbert ,  Andrew Gooley ,  Keith Williams

IPC: B01D57/02

CPC classification number: C07K1/285 ,  G01N27/44747 ,  G01N27/44795

Abstract: An improved method of separating a macromolecule by isoelectric focusing comprising subjecting the macromolecule to electrophoresis in an isoelectric-focusing medium including a substantially thiol-free reducing agent, preferably a trivalent phosphorous compound and more preferably tributyl phosphine, the improvement being the solubility and focusing of the macromolecule is enhanced compared to isoelectric focusing of the same macromolecule in a similar isoelectric-focusing medium containing a thiol-reducing agent.

Abstract translation: 通过等电聚焦分离大分子的改进方法包括使大分子在包括基本上不含硫醇的还原剂，优选三价磷化合物，更优选三丁基膦的等电聚焦介质中进行电泳，其改进是溶解度和聚焦 与含有硫醇还原剂的类似等电聚焦介质中的相同大分子的等电聚焦相比，大分子得到增强。

公开(公告)号：US20130330249A1

公开(公告)日：2013-12-12

申请号：US13898232

申请日：2013-05-20

Applicant: Ernest Frederick Dawes ,  Andrew Gooley

Inventor： Ernest Frederick Dawes ,  Andrew Gooley

IPC: G01N1/40

CPC classification number: G01N1/405 ,  G01N2001/1427

Abstract: A flow control assembly for use in preparation of samples for analysis by solid phase extraction includes a housing assembly having a first passage communicating with a needle bore and locating a solid phase sorbent, a second fluid port, and a valve for selective aspiration or delivery of fluid across a solid phase sorbent, or bypassing the solid phase sorbent. Independent flow paths are provided for a sample via the needle, and of the elution solvent via a separate port. The valve includes relatively slideable first and second members, the second member including an elongate shank that is relatively reciprocably slideable for effecting selective aspiration or delivery of fluid between first and second relative positions. A third fluid port may be provided communicating with the elongate second passage for selective fluid communication with a port in a shank when the shank is at the second relative position.

Abstract translation: 用于制备用于通过固相萃取分析的样品的流量控制组件包括壳体组件，其具有与针孔连通的第一通道并且定位固相吸附剂，第二流体端口和用于选择性抽吸或递送的阀 流过固相吸附剂，或绕过固相吸附剂。 通过针头和洗脱溶剂经由单独的端口为样品提供独立的流动路径。 所述阀包括相对可滑动的第一和第二构件，所述第二构件包括可相对往复滑动的细长柄，用于在第一和第二相对位置之间进行选择性抽吸或输送流体。 可以提供第三流体端口，其与细长的第二通道连通，用于当柄处于第二相对位置时与柄中​​的端口选择性地流体连通。

公开(公告)号：US20060243110A1

公开(公告)日：2006-11-02

申请号：US11409271

申请日：2006-04-24

Applicant: Dirk Ostermann ,  Cheng Bing ,  Pieter Faber ,  George Kraguljac ,  Andrew Gooley

Inventor： Dirk Ostermann ,  Cheng Bing ,  Pieter Faber ,  George Kraguljac ,  Andrew Gooley

IPC: B26F1/14

CPC classification number: G01N35/109 ,  B26D7/1818 ,  B26F1/16 ,  G01N1/04 ,  G01N27/44739 ,  Y10T83/2133 ,  Y10T83/9428

Abstract: A sampling device includes a table (12) and an overhead beam (14) which is spaced and supported above the table by two columns (16, 18). In the center of the beam, a cutting tool (20) assembly is located. The table is mounted so as to be moveable in the x and y directions in a generally horizontal plane. By moving the table in the x and y directions, any part of the table may be located under the cutting tool assembly. A computerized controller includes a digital scanner to record an image of an array of samples on the table and control the cutting tool to cause it to cut out a sample from the array, pick it up and transfer it to a selected location.

Abstract translation: 采样装置包括台（12）和塔架梁（14），其通过两列（16,18）间隔并支撑在工作台的上方。 在梁的中心，设有切割工具（20）组件。 桌子被安装成在大致水平的平面上在x和y方向上可移动。 通过在x和y方向上移动桌子，桌子的任何部分可能位于切割工具组件下方。 计算机化控制器包括数字扫描器，用于将样品阵列的图像记录在桌子上并控制切割工具以使其从阵列中切出样品，将其拾取并将其转移到选定的位置。