公开(公告)号：US06602696B1

公开(公告)日：2003-08-05

申请号：US09787583

申请日：2001-06-11

Applicant: Maartje Maria Franse ,  Catherine Marie Therese Grassin ,  Margareta Adriana Herweijer ,  Petrus Johannes Albertus Meeuwsen ,  Albert Johannes Joseph Van Ooijen ,  Alphons Gerard Joseph Voragen

Inventor： Maartje Maria Franse ,  Catherine Marie Therese Grassin ,  Margareta Adriana Herweijer ,  Petrus Johannes Albertus Meeuwsen ,  Albert Johannes Joseph Van Ooijen ,  Alphons Gerard Joseph Voragen

IPC: C07H2104

CPC classification number: C12Y302/01015 ,  C12N9/2402

Abstract: The present invention relates to a DNA sequence obtainable from Aspergillus tubigensis which encodes a polygalacturonase. It also relates to vectors comprising the DNA sequence and to host cells transformed with such vectors. The invention further relates to the expression of the polygalacturonase and its use.

Abstract translation: 本发明涉及可从编码多聚半乳糖醛酸酶的曲霉菌（Aspergillus tubigensis）获得的DNA序列。 它还涉及包含DNA序列的载体和用这些载体转化的宿主细胞。 本发明还涉及多聚半乳糖醛酸酶的表达及其用途。

公开(公告)号：US6033900A

公开(公告)日：2000-03-07

申请号：US201505

申请日：1998-11-30

Applicant: Lene Venke Kofod ,  Lene Nonboe Andersen ,  Henrik Dalb.o slashed.ge ,  Markus Sakari Kauppinen ,  Stephan Christgau ,  Hans Peter Heldt-Hansen ,  Claus Christophersen ,  Per Munk Nielsen ,  Alphons Gerard Joseph Voragen ,  Hendrik Arie Schols

Inventor： Lene Venke Kofod ,  Lene Nonboe Andersen ,  Henrik Dalb.o slashed.ge ,  Markus Sakari Kauppinen ,  Stephan Christgau ,  Hans Peter Heldt-Hansen ,  Claus Christophersen ,  Per Munk Nielsen ,  Alphons Gerard Joseph Voragen ,  Hendrik Arie Schols

IPC: A23K20/189 ,  A23L2/84 ,  A23L29/00 ,  C12N9/24 ,  C12N9/26 ,  C12N15/09 ,  C12N15/56 ,  C12R1/66 ,  C13B10/08 ,  C13K13/00 ,  C08B30/04 ,  A23B7/10 ,  C08B1/00

CPC classification number: C13B10/083 ,  A23K20/189 ,  A23L2/84 ,  A23L29/06 ,  C12N9/2402 ,  C12Y302/01171 ,  C13K13/00

Abstract: Animal feed compositions and methods for treating one or more of soy, pea or rape-seed, or other material derived from Fabales or Cruciferaceae, with an enzyme having rhamnogalacturonase activity, wherein the enzyme having rhamnogalacturonase activity cleaves a rhamnogalacturonan backbone to produce rhamnose as a non-reducing end (RGase II) or cleaves a rhamnogalaturonan backbone to produce galacturonic acid as a non-reducing end.

Abstract translation: 用具有鼠李糖聚半乳糖醛酸酶活性的酶处理一种或多种大豆，豌豆或油菜种子或其他衍生自法布尔斯或十字花科的物质的动物饲料组合物和方法，其中具有鼠李糖半乳糖醛酸酶活性的酶切割鼠李糖半乳糖醛酸骨架以产生鼠李糖 非还原末端（RGase II）或切割鼠李糖激肽主链以产生作为非还原末端的半乳糖醛酸。

公开(公告)号：US06492159B1

公开(公告)日：2002-12-10

申请号：US09319790

申请日：1999-07-19

Applicant: Jean Paul Vincken ,  Gerrit Beldman ,  Alphons Gerard Joseph Voragen

Inventor： Jean Paul Vincken ,  Gerrit Beldman ,  Alphons Gerard Joseph Voragen

IPC: C12N942

CPC classification number: A23L2/62

Abstract: An enzyme preparation composition comprising a mixture of enzymes comprising an enzyme with homogalacturonan activity, more specifically pectin lyase, and an enzyme with endoglucanase activity, wherein the enzymes are in relative proportions to produce cloud volume from fruit or vegetable material. The preparation is such that enzymes which reduce the cloud volume by more than 5% are excluded, and wherein the endoglucanase has both CMCase activity and xyloglucanase activity in a ratio of at least 0.7.

Abstract translation: 一种酶制剂组合物，其包含含有高半聚半乳糖醛酸活性的酶的混合物，更具体地说是果胶裂解酶和具有内切葡聚糖酶活性的酶的酶的混合物，其中所述酶以相对比例从水果或植物材料产生云量。 该制剂使得排除了将云体积减少5％以上的酶除外，其中内切葡聚糖酶具有至少0.7的比例的CMCase活性和木葡聚糖酶活性。

公开(公告)号：US5882911A

公开(公告)日：1999-03-16

申请号：US102433

申请日：1998-06-22

Applicant: Lene Venke Kofod ,  Lene Nonboe Andersen ,  Henrik Dalb.o slashed.ge ,  Markus Sakari Kauppinen ,  Stephan Christgau ,  Hans Peter Heldt-Hansen ,  Claus Christophersen ,  Per Munk Nielsen ,  Alphons Gerard Joseph Voragen ,  Hendrik Arie Schols

Inventor： Lene Venke Kofod ,  Lene Nonboe Andersen ,  Henrik Dalb.o slashed.ge ,  Markus Sakari Kauppinen ,  Stephan Christgau ,  Hans Peter Heldt-Hansen ,  Claus Christophersen ,  Per Munk Nielsen ,  Alphons Gerard Joseph Voragen ,  Hendrik Arie Schols

IPC: A23K20/189 ,  A23L2/84 ,  A23L29/00 ,  C12N9/24 ,  C12N9/26 ,  C12N15/09 ,  C12N15/56 ,  C12R1/66 ,  C13B10/08 ,  C13K13/00 ,  C07H21/04 ,  C12N1/14 ,  C12N15/00

CPC classification number: C13B10/083 ,  A23K20/189 ,  A23L2/84 ,  A23L29/06 ,  C12N9/2402 ,  C12Y302/01171 ,  C13K13/00

Abstract: The present invention relates to DNA sequences encoding a rhamnogalacturonase which comprises(a) the DNA sequence of nucleotides 64-1587 of SEQ ID NO:1;(b) a DNA sequence which hybridizes to the same probe as nucleotides 64-1587 of SEQ ID NO:1 under conditions of presoaking in 5.times.SSC and prehybridizing for 1 hour at -40.degree. C. in a solution of 5.times.SSC, 5.times.Denhardt's solution, 50 mM sodium phosphate, pH 6.8, and 50 mg of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with 50 .mu.Ci 32-P-dCTP labelled probe for 18 h at -40.degree. C., followed by washing three times in 2.times.SSC, 0.2% SDS at 40.degree. C. for 30 minutes; or(c) a DNA sequence encoding an amino acid sequence having amino acids 20-527 of the sequence of SEQ ID NO;2.

Abstract translation: 本发明涉及编码鼠李糖半乳糖醛酸酶的DNA序列，其包含（a）SEQ ID NO：1的核苷酸64-1587的DNA序列; （b）在5×SSC中预浸泡的条件下，在-40℃下在5×SSC，5×丹登溶液50μl的溶液中预杂交1小时，与SEQ ID NO：1的核苷酸64-1587相同的探针杂交的DNA序列 mM磷酸钠，pH 6.8和50mg变性超声小牛胸腺DNA，然后在补充有50μCi32-P-dCTP标记的探针的相同溶液中在-40℃下杂交18小时，然后洗涤三次 次，2xSSC，0.2％SDS，40℃30分钟; 或（c）编码具有SEQ ID NO：2的序列的氨基酸20-527的氨基酸序列的DNA序列。