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公开(公告)号：US5869299A
公开(公告)日：1999-02-09
申请号：US446806
申请日：1995-08-25
Applicant: Keith Howard Baggaley , Neville Hubert Nicholson , Stephen William Elson , Jeffrey Edwards , Alison Jane Earl , William Henry Holms , David Michael Mousdale , Jack Edward Baldwin , Christopher Schofield
Inventor： Keith Howard Baggaley , Neville Hubert Nicholson , Stephen William Elson , Jeffrey Edwards , Alison Jane Earl , William Henry Holms , David Michael Mousdale , Jack Edward Baldwin , Christopher Schofield
IPC: C12N15/09 , C07C257/14 , C07C279/14 , C07D205/08 , C07D503/00 , C12N9/06 , C12N9/14 , C12N9/70 , C12N9/78 , C12N15/00 , C12N15/57 , C12P17/10 , C12P17/18 , C12R1/465 , C12N9/80
CPC classification number: C07C279/14 , C07D205/08 , C12N9/78 , C12P17/10 , C12P17/188
Abstract: A process for preparing clavulanic acid and other clavam derivatives using an enzyme system from Streptomyces, particularly S. Clavuligerus.
Abstract translation: PCT No.PCT / GB93 / 02442 Sec。 371日期1995年8月25日 102（e）日期1995年8月25日PCT 1993年11月26日PCT公布。 公开号WO94 / 12654 日期1994年6月9日使用来自链霉菌（Streptomyces），特别是S. Clavuligerus的酶系制备克拉维酸和其他克拉维姆衍生物的方法。
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2.发明授权
公开(公告)号：US5759831A
公开(公告)日：1998-06-02
申请号：US159950
申请日：1993-12-01
Applicant: John Edward Hodgson , Alison Jane Earl
Inventor： John Edward Hodgson , Alison Jane Earl
CPC classification number: C12N15/52 , C12P17/188
Abstract: DNA sequences obtained from S. clavuligerus, recombinant vectors incorporating such sequences and hosts transformed with such vectors are disclosed. The DNA comprises one or more genes encoding one or more enzymes involved in the biosynthesis of clavulanic acid and may be used to improve the yield of clavulanic acid produced by clavulanic acid-producing organisms such as S. clavuligerus ATCC 27064.
Abstract translation: 公开了从S.clavuligerus获得的DNA序列，结合这样的序列的重组载体和用这些载体转化的宿主。 DNA包含编码参与克拉维酸生物合成的一种或多种酶的一种或多种基因，并且可用于提高由克拉维酸产生生物体如克拉维氏菌ATCC 27064产生的克拉维酸的产量。
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公开(公告)号：US06258555B1
公开(公告)日：2001-07-10
申请号：US08909108
申请日：1997-08-11
Applicant: Martin Karl Russell Burnham , Alison Jane Earl , John Henry Bull , David John Smith , Geoffrey Turner
Inventor： Martin Karl Russell Burnham , Alison Jane Earl , John Henry Bull , David John Smith , Geoffrey Turner
IPC: C12P3700
Abstract: DNA encoding the gene for the synthetase enzyme capable of generating &dgr; (L-a-aminoadipyl)-L-crysteinyl-D-valine (ACV) from its constituent amino acids was obtained from several penicillin and cephalosporin producing organisms, e.g. Penicillium chrysogenum, cephalosporium and a Flavobacterium species. The DNA was used to prepare recombinant vectors comprising the ACV synthetase gene and hosts transformed with such vectors. The ACV synthetase gene can form part of a gene cluster comprising other genes involved in -&bgr;-lactam biosynthesis and the production of penicillin by expression of the entire biosynthetic gene cluster for the synthesis of penicillin from primary amino acids is described. Suitable hosts in which expression can take place include heterologous hosts which are naturally non-producers of penicillin.
Abstract translation: 编码能够从其组成氨基酸产生δ（L-α-氨基己二酰基）-L-结晶乙酰-D-缬氨酸（ACV）的合成酶的基因的DNA从几种青霉素和头孢菌素生产生物获得，例如， 产黄青霉，头孢菌属和黄杆菌属。 使用该DNA制备包含ACV合成酶基因的重组载体和用这些载体转化的宿主。 ACV合成酶基因可以形成包含参与β-内酰胺生物合成的其他基因的基因簇的一部分，并且通过用于从初级氨基酸合成青霉素的整个生物合成基因簇的表达来产生青霉素。 可以进行表达的合适的宿主包括天然非青霉素生产者的异源宿主。
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公开(公告)号：US06361982B1
公开(公告)日：2002-03-26
申请号：US08491944
申请日：1995-08-31
Applicant: Nicola Jane Walters , Barry Barton , Alison Jane Earl
Inventor： Nicola Jane Walters , Barry Barton , Alison Jane Earl
IPC: C12D740
CPC classification number: C12P17/188 , A61K38/00 , C07K14/36
Abstract: DNA comprising a regulatory gene for clavulanic acid biosynthesis. DNA is no greater than 7 kb in length and has the configuration of restriction sites shown in FIG. 1 or a fragment thereof. Use of DNA in a method for producing clavulanic acid in a host.
Abstract translation: 包含克拉维酸生物合成调节基因的DNA。 DNA长度不大于7kb，具有图1所示的限制性位点的构型。 1或其片段。 在一个宿主生产克拉维酸的方法中使用DNA。
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