公开(公告)号：US20250002892A1

公开(公告)日：2025-01-02

申请号：US18709663

申请日：2022-11-22

Applicant: AMANO ENZYME INC.

Inventor： Hirotaka Matsubara

IPC: C12N9/80 ,  C12N15/70 ,  C12P21/00 ,  C12R1/19

Abstract: A protein-deamidating enzyme includes a polypeptide that contains the amino acid sequence given by SEQ ID NO: 1 or a partial sequence thereof (the amino acid sequence of positions 1 to 221 in SEQ ID NO: 1, the amino acid sequence of positions 36 to 231 in SEQ ID NO: 1, or the amino acid sequence of positions 36 to 221 in SEQ ID NO: 1); a polypeptide that has a protein glutaminase activity and contains an amino acid sequence obtained by substituting, adding, inserting, or deleting one or a plurality of amino acids in the amino acid sequence given by SEQ ID NO: 1 or the partial sequences thereof; or a polypeptide that has a protein glutaminase activity and contains an amino acid sequence that has a 70% or higher sequence identity with the amino acid sequence given by SEQ ID NO: 1 or the partial sequences thereof.

公开(公告)号：US20240306668A1

公开(公告)日：2024-09-19

申请号：US18264853

申请日：2022-02-10

Applicant: AMANO ENZYME INC.

Inventor： Kiyota SAKAI

IPC: A23J3/16 ,  A23J3/18 ,  A23J3/22 ,  A23L5/43 ,  A23L29/231 ,  A23L29/262

CPC classification number: A23J3/16 ,  A23J3/18 ,  A23J3/227 ,  A23L5/43 ,  A23L29/231 ,  A23L29/262

Abstract: The purpose of the present invention is to provide a new processing technique to increase the crosslinkability of a plant protein material. In this invention, causing polysaccharides and multi-copper oxidase to act on a plant protein can increase the crosslinking effect of the plant protein.

公开(公告)号：US20240301463A1

公开(公告)日：2024-09-12

申请号：US17906217

申请日：2021-03-12

Applicant: Amano Enzyme Inc.

Inventor： Kazunori YOSHIDA ,  Yukihide SATO ,  Tomoshi KAMEDA ,  Jinzen IKEBE

IPC: C12P41/00 ,  C12N9/20 ,  C12N15/70 ,  C12P7/22

CPC classification number: C12P41/004 ,  C12N9/20 ,  C12N15/70 ,  C12P7/22

Abstract: Provided is a technique that can further improve substrate specificity to an L-form during L-menthol and/or L-menthol ester production. A polypeptide that: has A120G, Q88A, Q88G, Q88D, Q88M, Q88L variants of a polypeptide comprising the amino acid sequence represented by sequence no. 1, and in said variants, has a random different moiety other than an amino-acid residue into which a substitution has been introduced; has esterification activity for L-menthol and/or hydrolyzing activity for L-menthol ester; and improves substrate specificity to L-menthol and/or L-menthol ester compared to a polypeptide comprising the amino acid sequence represented by sequence no. 1. During L-menthol and/or L-menthol ester production, the polypeptide can further improve the optical purity of the product.

公开(公告)号：US20240138431A1

公开(公告)日：2024-05-02

申请号：US18547980

申请日：2022-02-25

Applicant: AMANO ENZYME U.S.A. CO., LTD. ,  AMANO ENZYME INC.

Inventor： Keita OKUDA

IPC: A23C20/02 ,  A23J3/14 ,  A23J3/34

CPC classification number: A23C20/025 ,  A23J3/14 ,  A23J3/346

Abstract: The purpose of the present invention is to provide a technique for producing a cheese substitute by which improved stretchability can be imparted to a cheese substitute which contains a plant protein and 0.6 part by weight or more, per part by weight of the plant protein, of starch. A method for producing a stretching cheese substitute, said method comprising a step for treating a starting material composition, which contains a plant protein and 0.6 part by weight or more, per part by weight of the plant protein, of starch, with an enzyme preparation containing a protease optionally together with an amylase, wherein the enzyme preparation is used so as to give a starch gelatinization ability of the amylase of 8 U or less per gram of the starch, relative to 100,000 U of the protease activity of the protease per gram of the plant protein. The cheese substitute obtained by this method has improved stretchability.

公开(公告)号：US20240114915A1

公开(公告)日：2024-04-11

申请号：US18275792

申请日：2022-02-04

Applicant: AMANO ENZYME U.S.A. Co., LTD. ,  AMANO ENZYME INC.

Inventor： Keita OKUDA ,  Miho NAGAYA

IPC: A23C19/032

CPC classification number: A23C19/0328

Abstract: The purpose of the present invention is to provide a technology for promoting short-chain fatty acid separation in a natural cheese production technology employing lipase of microbial origin. It is possible to promote short-chain fatty acid separation by means of a natural cheese production method including a step for causing lipase of microbial origin and protease of filamentous fungal origin to act on milk.

公开(公告)号：US20240084278A1

公开(公告)日：2024-03-14

申请号：US18503526

申请日：2023-11-07

Applicant: AMANO ENZYME INC.

Inventor： Yukiko Hoshi ,  Masamichi Okada ,  Akio Horii ,  Masayuki Hojo

IPC: C12N9/38 ,  C07H3/06 ,  C12N5/10 ,  C12N15/09 ,  C12P19/00 ,  C12P19/14

CPC classification number: C12N9/2471 ,  C07H3/06 ,  C12N5/10 ,  C12N15/09 ,  C12P19/00 ,  C12P19/14 ,  C12Y302/01023

Abstract: The present invention has a purpose of providing a novel β-galactosidase enzyme useful for the production of oligosaccharides. Disclosed is a β-galactosidase enzyme comprising the amino acid sequence of any one of SEQ ID NOs: 1 to 4 or an amino acid sequence that is 80% or more identical to said amino acid sequence.

公开(公告)号：US20240057625A1

公开(公告)日：2024-02-22

申请号：US18278896

申请日：2022-02-25

Applicant: AMANO ENZYME INC.

Inventor： Masahiro KAMON ,  Shusaku TADA ,  Dai HASHIMURA ,  Kan YAMASHIRO ,  Takuma HAYASHI

IPC: A23C9/12 ,  C12N9/04 ,  C12N9/42 ,  A23C9/123 ,  A23C11/10 ,  A23C9/127

CPC classification number: A23C9/1213 ,  C12N9/0006 ,  C12Y101/00 ,  C12N9/2445 ,  C12Y302/01021 ,  A23C9/123 ,  A23C11/106 ,  A23C11/10 ,  A23C9/1275

Abstract: The main purpose of this technique is to newly provide a technique whereby it becomes possible to shorten the time for anaerobic fermentation by reducing the concentration of oxygen contained in a raw material by a method other than an inert gas replacement method that requires the introduction of a facility. This technique provides a method for producing a fermented food or beverage, including a saccharide oxidase action step for allowing a saccharide oxidase to act on a portion or the whole of a saccharide in a raw material, and an anaerobic fermentation step for performing anaerobic fermentation. This technique also provides an oxygen concentration reducing agent for anaerobic fermentation use which contains a saccharide oxidase. This technique further provides an anaerobic fermentation method which includes a saccharide oxidase action step for allowing a saccharide oxidase to act on a portion or the whole of a saccharide in a raw material.

公开(公告)号：US20240049746A1

公开(公告)日：2024-02-15

申请号：US18259265

申请日：2021-12-23

Applicant: AMANO ENZYME INC. ,  Amano Enzyme Asia Pacific Co., Ltd. ,  AMANO ENZYME EUROPE LTD.

Inventor： Supaporn NAKNUKOOL ,  Hiroki FUJIOKA

IPC: A23J3/14 ,  A23L2/66 ,  A23L2/38

CPC classification number: A23J3/14 ,  A23L2/66 ,  A23L2/38

Abstract: The purpose of the present invention is to provide a technique for producing a rice protein-containing liquid composition having improved smoothness. The rice protein-containing liquid composition, obtained by a method which is for producing a rice protein-containing liquid composition and comprises a step for treating a rice protein-containing liquid material with a protein deamidase, has improved smoothness.

公开(公告)号：US20240008515A1

公开(公告)日：2024-01-11

申请号：US18254697

申请日：2021-11-25

Applicant: AMANO ENZYME INC.

Inventor： Kiyota SAKAI

IPC: A23L29/20 ,  A23L15/00 ,  C12N9/80

CPC classification number: A23L29/20 ,  A23L15/25 ,  C12N9/80 ,  C12Y305/01044

Abstract: The purpose of the present invention is to provide a technology that enables control for increasing or decreasing the heat coagulation gel strength of egg white protein, and that has little effect on taste. This heat coagulation gel strength regulating agent for egg white protein contains a protein deamidase and can control the increase or decrease of the heat coagulation gel strength of egg white protein.

公开(公告)号：US11859222B2

公开(公告)日：2024-01-02

申请号：US17961625

申请日：2022-10-07

Applicant: AMANO ENZYME INC.

Inventor： Yukiko Hoshi ,  Masamichi Okada ,  Akio Horii ,  Masayuki Hojo

IPC: C12N9/38 ,  C12P19/00 ,  C07H3/06 ,  C12N5/10 ,  C12N15/09 ,  C12P19/14

CPC classification number: C12N9/2471 ,  C07H3/06 ,  C12N5/10 ,  C12N15/09 ,  C12P19/00 ,  C12P19/14 ,  C12Y302/01023

Abstract: The present invention has a purpose of providing a novel β-galactosidase enzyme useful for the production of oligosaccharides. Disclosed is a β-galactosidase enzyme comprising the amino acid sequence of any one of SEQ ID NOs: 1 to 4 or an amino acid sequence that is 80% or more identical to said amino acid sequence.