公开(公告)号：US10018602B2

公开(公告)日：2018-07-10

申请号：US15032394

申请日：2014-10-27

申请人： SHIMADZU CORPORATION ,  AICHI PREFECTURAL GOVERNMENT

发明人： Eiji Ueno ,  Riki Kitano ,  Haruhiko Miyagawa

IPC分类号： G01N30/06 ,  G01N30/88 ,  G01N30/86 ,  B01J20/281 ,  B01J20/283 ,  G01N30/72 ,  G01N30/00

CPC分类号： G01N30/06 ,  B01J20/283 ,  B01J2220/54 ,  G01N30/48 ,  G01N30/7206 ,  G01N30/8665 ,  G01N30/88 ,  G01N2030/486

摘要： A number of analytes are divided into groups based on at least either the elution position determined by silica gel column chromatography under the same condition or the partition ratio in a hexane-acetonitrile partition method. To each group, a compound obtained by labeling one of the compounds in the group by deuterium and/or carbon is assigned as the surrogate. After those surrogates are added as internal standard substances to a standard sample for the creation of calibration curves, a GC/MS analysis for the sample is performed and a calibration curve is created for each analyte. In the measurement of an unknown sample, the same set of surrogates are added to the sample and the GC/MS analysis is performed. A quantitative determination processor determines the quantity of each analyte by comparing a peak area ratio calculated from the analysis result with a calibration curve read from the database.

公开(公告)号：US20160266074A1

公开(公告)日：2016-09-15

申请号：US15032394

申请日：2014-10-27

申请人： SHIMADZU CORPORATION ,  AICHI PREFECTURAL GOVERNMENT

发明人： Eiji UENO ,  Riki KITANO ,  Haruhiko MIYAGAWA

IPC分类号： G01N30/06 ,  B01J20/281

CPC分类号： G01N30/06 ,  B01J20/283 ,  B01J2220/54 ,  G01N30/48 ,  G01N30/7206 ,  G01N30/8665 ,  G01N30/88 ,  G01N2030/486

摘要： A number of analytes are divided into groups based on at least either the elution position determined by silica gel column chromatography under the same condition or the partition ratio in a hexane-acetonitrile partition method. To each group, a compound obtained by labeling one of the compounds in the group by deuterium and/or carbon is assigned as the surrogate. After those surrogates are added as internal standard substances to a standard sample for the creation of calibration curves, a GC/MS analysis for the sample is performed and a calibration curve is created for each analyte. In the measurement of an unknown sample, the same set of surrogates are added to the sample and the GC/MS analysis is performed. A quantitative determination processor determines the quantity of each analyte by comparing a peak area ratio calculated from the analysis result with a calibration curve read from the database.

摘要翻译： 至少基于相同条件下的硅胶柱色谱法测定的洗脱位置或在己烷 - 乙腈分配方法中的分配比例将多种分析物分成几组。 对于各组，将通过氘和/或碳标记该组中的化合物之一而获得的化合物被指定为替代物。 将这些替代物作为内标物质添加到标准样品中以创建校准曲线后，对样品进行GC / MS分析，并为每个分析物创建校准曲线。 在未知样品的测量中，将相同的替代品组添加到样品中，并进行GC / MS分析。 定量确定处理器通过将从分析结果计算的峰面积比与从数据库读取的校准曲线进行比较来确定每个分析物的量。