公开(公告)号：US20170242026A1

公开(公告)日：2017-08-24

申请号：US15328073

申请日：2015-07-21

Applicant: INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM) ,  UNIVERSITE D'EVRY - VAL D'ESSONNE

Inventor： David PASTRE ,  Michel CAILLERET ,  Patrick CURMI ,  Mirela BOCA

IPC: G01N33/68 ,  C12N15/10 ,  C07K14/47

CPC classification number: G01N33/6845 ,  C07K14/47 ,  C07K2319/20 ,  C07K2319/30 ,  C07K2319/60 ,  C07K2319/70 ,  C12N15/1055 ,  G01N33/582 ,  G01N2333/47 ,  C12Q2563/107

Abstract: A method for detecting an interaction between one or more protein bait and one or more candidate prey in a eukaryotic cell, comprising the steps of: a) providing an eukaryotic cell expressing (i) one or more protein bait, and (ii) one or more candidate prey, wherein said protein bait comprises a bait moiety and a polymerized-tubulin binding moiety. b) determining the occurence of an interaction between said one or more protein bait and said one or more candidate prey in the eukaryotic cell, wherein said protein bait is bound to polymerized tubulin in the eukaryotic cell, thereby localizing said one or more candidate prey along said polymerized tubulin, thereby detecting said interaction.

公开(公告)号：US20160377610A1

公开(公告)日：2016-12-29

申请号：US15149833

申请日：2016-05-09

Applicant: GENOPTICS ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  UNIVERSITE D'EVRY VAL D'ESSONNE

Inventor： Régis DANIEL ,  Florence GONNET ,  William BUCHMANN ,  Sophie BELLON ,  Nathalie JARROUX ,  Marielle ANGER-LEROY

IPC: G01N33/543 ,  G01N33/68 ,  G01N21/552 ,  G01N33/553

CPC classification number: G01N33/54373 ,  G01N21/554 ,  G01N33/54353 ,  G01N33/553 ,  G01N33/6851 ,  G01N2560/00 ,  G01N2570/00 ,  Y10T436/24

Abstract: The invention relates to a method for coupling in-line the analysis of molecular interactions by surface plasmon resonance (SPR) with a structural identification by mass spectrometry using the same functionalized support for both types of analysis.

Abstract translation: 本发明涉及一种通过表面等离子体共振（SPR）在线分析分子相互作用的分析方法，并通过质谱法进行结构鉴定，使用相同的功能化支持进行两种分析。

公开(公告)号：US20040009505A1

公开(公告)日：2004-01-15

申请号：US10400985

申请日：2003-03-27

Applicant: Genethon III and Universite D'Evry Val D'Essonne

Inventor： Gilles Waksman ,  Jerome Lamartine

IPC: C12Q001/68 ,  A01K067/00 ,  C07H021/04 ,  A61K007/06 ,  A61K048/00 ,  A61K007/11 ,  C12N009/64

CPC classification number: C12Q1/6883 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2227/50 ,  A01K2267/0306 ,  A61K38/00 ,  A61K48/00 ,  C07K14/705 ,  C12N15/8509 ,  C12Q2600/158

Abstract: The invention is derived from the identification of mutations in the GJB6 gene, responsible for Clouston's syndrome. The symptomatology of said syndrome suggests that the GJB6 coding for connexin 30 (Cx-30), is most probably involved also in other types of alopecia with genetic susceptibility, in particular non-pathological. Therefore, the invention concerns the GJB6 gene sequence bearing at least one of the mutations 31 (G>A) and 263 (C>T), responsible for Clouston's syndrome, and the use of constructs comprising the GJB6 gene, both for preparing pharmaceutical compositions for treating Clouston's syndrome and/or certain disorders of the body hair system, and for screening molecules likely to have a beneficial effect in the treatment of alopecia. The invention also concerns methods for diagnosing Clouston's syndrome.

Abstract translation: 本发明来源于鉴定GJB6基因中的突变，导致克劳斯顿综合征。 所述综合征的症状学表明，编码连接蛋白30（Cx-30）的GJB6最可能也涉及具有遗传易感性的其他类型的脱发，特别是非病理性的。 因此，本发明涉及带有至少一个负责克劳斯顿综合征的突变31（G> A）和263（C> T）的GJB6基因序列，以及包含GJB6基因的构建体的用途，用于制备药物组合物 用于治疗克劳斯顿综合征和/或身体毛发系统的某些障碍，以及用于筛选可能在治疗脱发中有益的分子。 本发明还涉及诊断克劳斯顿综合征的方法。