公开(公告)号：US10807937B2

公开(公告)日：2020-10-20

申请号：US16339756

申请日：2016-07-11

申请人： CATHAY BIOTECH INC. ,  CIBT AMERICA INC.

发明人： Bingbing Qin ,  Yufeng Yang ,  Xiucai Liu

IPC分类号： C07C51/43 ,  C07C51/42 ,  C07C55/21 ,  C12P7/64 ,  C07C51/02 ,  C07C51/347 ,  C12P7/44

摘要： The present disclosure provides a method for isolating a long chain dicarboxylic acid from fermentation broth containing microbial cells. Also provided is a substantially pure long chain dicarboxylic acid isolated by the method.

公开(公告)号：US10759737B2

公开(公告)日：2020-09-01

申请号：US16075929

申请日：2016-09-07

申请人： Cathay Biotech Inc. ,  CIBT America Inc.

发明人： Xiucai Liu ,  Bingbing Qin ,  Chen Yang

IPC分类号： C07C209/86 ,  C12P13/00 ,  C07C211/09 ,  C07C209/84 ,  B01D3/10 ,  B01D11/04

摘要： Provided is a method for extracting a 1,5-pentanediamine from a solution system containing a 1,5-pentanediamine salt. The method comprises adding to the solution system an insoluble basic substance to form a solution system containing free 1,5-pentanediamine. The provided method has high applicability, is easy to use, and is environmentally-friendly, significantly lowering raw material costs and operating costs for the entire manufacturing process. The method achieves a high recovery rate for pentanediamine, and is more suitable for industrial-scale production.

公开(公告)号：US20200239919A1

公开(公告)日：2020-07-30

申请号：US16826606

申请日：2020-03-23

申请人： Cathay Biotech Inc. ,  CIBT America Inc.

发明人： Wenbo LIU ,  Min XU ,  Chen YANG ,  Howard CHOU ,  Xiucai LIU

IPC分类号： C12P7/64 ,  C12N9/02

摘要： The invention relates to a long-chain dibasic acid with low content of monobasic acid impurity and a production method thereof, in particular to the preparation of a long-chain dibasic acid producing strain by means of directed evolution and homologous recombination, and to the production of a long-chain dibasic acid with low content of monobasic acid impurity by fermentation of said strain. The invention relates to a mutated CYP52A12 gene, homologous gene or variant thereof, which, relative to GenBank Accession Number AY230498 and taking the first base upstream of the start codon ATG as −1, comprises a mutation. The invention relates to a strain comprising said mutated CYP52A12 gene, homologous gene or variant thereof wherein when the strain is fermented to produce a long-chain dibasic acid, the content of monobasic acid impurity in the fermentation product is significantly reduced.

公开(公告)号：US20200231999A1

公开(公告)日：2020-07-23

申请号：US16473149

申请日：2016-12-30

申请人： CATHAY R&D CENTER CO., Ltd. ,  CIBT AMERICA INC.

发明人： Howard CHOU ,  Wenqiang LU ,  Ling CHEN ,  Xiucai LIU

IPC分类号： C12P13/00 ,  C12N9/88

摘要： The invention provides CadA polypeptides with mutations that increase activity in alkaline pH compared to the wild-type lysine decarboxylase. The invention also provides methods of generating such mutant polypeptides, microorganisms genetically modified to overexpress the mutant polypeptides, and methods of generating such microorganism.