公开(公告)号：US08709785B2

公开(公告)日：2014-04-29

申请号：US13600109

申请日：2012-08-30

Applicant: Marguerite A. Cervin ,  Gopal K. Chotani ,  Frank J. Feher ,  Richard La Duca ,  Joseph C. McAuliffe ,  Andrei Miasnikov ,  Caroline M. Peres ,  Aaron S. Puhala ,  Karl J. Sanford ,  Fernando Valle ,  Gregory M. Whited

Inventor： Marguerite A. Cervin ,  Gopal K. Chotani ,  Frank J. Feher ,  Richard La Duca ,  Joseph C. McAuliffe ,  Andrei Miasnikov ,  Caroline M. Peres ,  Aaron S. Puhala ,  Karl J. Sanford ,  Fernando Valle ,  Gregory M. Whited

IPC: C12N1/00 ,  C12N1/20 ,  C12N15/00 ,  C12P5/02 ,  C07H21/04

CPC classification number: C12P5/007 ,  C12N9/1022 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y203/01009 ,  C12Y207/01036 ,  C12Y401/01033 ,  C12Y402/03027 ,  C12Y503/03002 ,  Y02E50/343

Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Abstract translation: 本发明的特征在于从培养细胞产生异戊二烯的方法。 本发明还提供包括这些培养细胞的组合物。

公开(公告)号：US08192959B2

公开(公告)日：2012-06-05

申请号：US10420587

申请日：2003-04-22

Applicant: Mark S. Payne ,  Stephen K. Picataggio ,  Amy K. Hsu ,  Ramesh V. Nair ,  Fernando Valle ,  Philippe Soucaille ,  Donald E. Trimbur

Inventor： Mark S. Payne ,  Stephen K. Picataggio ,  Amy K. Hsu ,  Ramesh V. Nair ,  Fernando Valle ,  Philippe Soucaille ,  Donald E. Trimbur

IPC: C12P19/34 ,  C12N15/00 ,  C12N15/74

CPC classification number: C07K14/36 ,  C12N9/90 ,  C12N15/70 ,  C12N15/76 ,  C12P7/18

Abstract: This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

Abstract translation: 本发明提供了一系列含有限制性内切核酸酶识别位点的低拷贝数质粒，所述限制性内切核酸酶识别位点用于克隆至少三种不同的基因或操纵子，每个侧翼有终止子序列，所述质粒含有不同水平的蛋白质表达的葡萄糖异构酶启动子的变体。 这些材料和方法可用于微生物的遗传工程，特别是在寻求多种遗传插入的情况下。

公开(公告)号：US08034583B2

公开(公告)日：2011-10-11

申请号：US12141522

申请日：2008-06-18

Applicant: Timothy C. Dodge ,  Manoj Kumar ,  M. Harunur Rashid ,  Fernando Valle

Inventor： Timothy C. Dodge ,  Manoj Kumar ,  M. Harunur Rashid ,  Fernando Valle

IPC: C12P21/06

CPC classification number: C12P7/58 ,  C07K14/24 ,  C12P7/60

Abstract: The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.

Abstract translation: 本发明涉及在细菌宿主细胞中工程代谢途径，其导致用于生产抗坏血酸（ASA）中间体的增强的碳流。 特别地，本发明涉及通过增加由内源性葡萄糖酸转运蛋白基因失活产生的葡萄糖酸盐的可利用性来增加细菌细胞中ASA中间体的产生。

公开(公告)号：US20110046014A1

公开(公告)日：2011-02-24

申请号：US12940225

申请日：2010-11-05

Applicant: MARK S. PAYNE ,  Stephen K. Picataggio ,  Amy Kuang-Hua Hsu ,  Ramesh V. Nair ,  Fernando Valle ,  Philippe Soucaille ,  Donald E. Trimbur

Inventor： MARK S. PAYNE ,  Stephen K. Picataggio ,  Amy Kuang-Hua Hsu ,  Ramesh V. Nair ,  Fernando Valle ,  Philippe Soucaille ,  Donald E. Trimbur

IPC: C40B40/08 ,  C07H21/00 ,  C12N15/63 ,  C07H21/04 ,  C12N1/21

CPC classification number: C07K14/36 ,  C12N9/90 ,  C12N15/70 ,  C12N15/76 ,  C12P7/18

Abstract: This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

Abstract translation: 本发明提供了一系列含有限制性内切核酸酶识别位点的低拷贝数质粒，所述限制性内切核酸酶识别位点用于克隆至少三种不同的基因或操纵子，每个侧翼有终止子序列，所述质粒含有不同水平的蛋白质表达的葡萄糖异构酶启动子的变体。 这些材料和方法可用于微生物的遗传工程，特别是在寻求多种遗传插入的情况下。

公开(公告)号：US20100285567A1

公开(公告)日：2010-11-11

申请号：US12664357

申请日：2008-06-02

Applicant: Kai Bao ,  Fernando Valle

Inventor： Kai Bao ,  Fernando Valle

IPC: C12N9/14 ,  C12N1/21 ,  C12N15/63 ,  C07H21/04

CPC classification number: C12P21/02 ,  C07K2319/02 ,  C12N9/16 ,  C12N15/625 ,  C12N15/76

Abstract: This invention provides polynucleotides encoding TAT fusion proteins, and methods for producing proteins of interest in a host cell. In particular, the present invention relates to polynucleotides, vectors, polypeptides and methods for expressing organophosphate-degrading enzymes e.g. organophosphorus hydrolase (OPH) in host cell, such as a Streptomyces species host cell.

Abstract translation: 本发明提供了编码TAT融合蛋白的多核苷酸，以及在宿主细胞中产生感兴趣的蛋白质的方法。 特别地，本发明涉及用于表达有机磷酸酯降解酶的多核苷酸，载体，多肽和方法，例如 有机磷水解酶（OPH）在宿主细胞中，如链霉菌属宿主细胞。

公开(公告)号：US20100274033A1

公开(公告)日：2010-10-28

申请号：US12768419

申请日：2010-04-27

Applicant: Fernando Sanchez-Riera ,  Stephen Del Cardayre ,  Fernando Valle

Inventor： Fernando Sanchez-Riera ,  Stephen Del Cardayre ,  Fernando Valle

IPC: C12P7/64 ,  C07C69/003 ,  C12N1/21

CPC classification number: C12P7/649 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/52 ,  C12P7/065 ,  Y02E50/13 ,  Y02E50/17

Abstract: Methods of producing fatty acid esters, such as fatty acid ethyl esters, from genetically engineered microorganisms are described.

Abstract translation: 描述了从遗传工程化的微生物生产脂肪酸酯，如脂肪酸乙酯的方法。

公开(公告)号：US20100257778A1

公开(公告)日：2010-10-14

申请号：US12758001

申请日：2010-04-11

Applicant: Alfred Gaertner ,  Andreas Schirmer ,  Fernando Valle ,  Stephen Del Cardayre

Inventor： Alfred Gaertner ,  Andreas Schirmer ,  Fernando Valle ,  Stephen Del Cardayre

IPC: C10L1/19 ,  C12P7/62 ,  C07C69/003 ,  C12P7/64 ,  C12N1/00 ,  C12N1/21 ,  C07C53/00

CPC classification number: C10L1/026 ,  C10L2200/0476 ,  C10L2270/026 ,  C10L2290/26 ,  C12P7/649 ,  Y02E50/13 ,  Y02P30/20

Abstract: Methods and compositions for producing fatty acid derivatives, for example, fatty esters, are described.

Abstract translation: 描述了用于生产脂肪酸衍生物的方法和组合物，例如脂肪酸酯。

公开(公告)号：US07745184B2

公开(公告)日：2010-06-29

申请号：US12053991

申请日：2008-03-24

Applicant: Marguerite A. Cervin ,  Philippe Soucaille ,  Fernando Valle

Inventor： Marguerite A. Cervin ,  Philippe Soucaille ,  Fernando Valle

IPC: C12P7/18 ,  C12P21/06 ,  C12Q1/68 ,  C12N1/21 ,  C12N15/74 ,  C07H21/04

CPC classification number: C12P7/18 ,  C12P7/20

Abstract: The present invention provides a microorganism useful for biologically producing 1,3-propanediol from a fermentable carbon source at higher yield than was previously known. The complexity of the cofactor requirements necessitates the use of a whole cell catalyst for an industrial process that utilizes this reaction sequence to produce 1,3-propanediol. The invention provides a microorganism with disruptions in specified genes and alterations in the expression levels of specified genes that is useful in a higher yielding process to produce 1,3-propanediol.

Abstract translation: 本发明提供了一种可用于从可发酵碳源生产1,3-丙二醇的微生物，其产率高于之前已知的。 辅助因子要求的复杂性需要使用全细胞催化剂用于工业过程，其利用该反应顺序产生1,3-丙二醇。 本发明提供了一种在特定基因中具有破坏的微生物，并且在用于产生1,3-丙二醇的更高产率的方法中有用的特定基因的表达水平的改变。

公开(公告)号：US20090203102A1

公开(公告)日：2009-08-13

申请号：US12335071

申请日：2008-12-15

Applicant: Marguerite A. Cervin ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. Mcauliffe ,  Frank J. Feher ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

Inventor： Marguerite A. Cervin ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. Mcauliffe ,  Frank J. Feher ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

IPC: C12P5/02 ,  C12N1/20 ,  C12N1/21

CPC classification number: C12P5/007 ,  C12N9/1022 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y203/01009 ,  C12Y207/01036 ,  C12Y401/01033 ,  C12Y402/03027 ,  C12Y503/03002 ,  Y02E50/343

Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Abstract translation: 本发明的特征在于从培养细胞产生异戊二烯的方法。 本发明还提供包括这些培养细胞的组合物。

公开(公告)号：US20090156430A1

公开(公告)日：2009-06-18

申请号：US12370864

申请日：2009-02-13

Applicant: Mark S. Payne ,  Stephen K. Picataggio ,  Amy Kuang-Hua Hsu ,  Ramesh V. Nair ,  Fernando Valle ,  Philippe Soucaille ,  Donald E. Trimbur

Inventor： Mark S. Payne ,  Stephen K. Picataggio ,  Amy Kuang-Hua Hsu ,  Ramesh V. Nair ,  Fernando Valle ,  Philippe Soucaille ,  Donald E. Trimbur

IPC: C40B40/08 ,  C12N15/11 ,  C12N15/00

CPC classification number: C07K14/36 ,  C12N9/90 ,  C12N15/70 ,  C12N15/76 ,  C12P7/18

Abstract: This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

Abstract translation: 本发明提供了一系列含有限制性内切核酸酶识别位点的低拷贝数质粒，所述限制性内切核酸酶识别位点用于克隆至少三种不同的基因或操纵子，每个侧翼有终止子序列，所述质粒含有不同水平的蛋白质表达的葡萄糖异构酶启动子的变体。 这些材料和方法可用于微生物的遗传工程，特别是在寻求多种遗传插入的情况下。