公开(公告)号：US20210172014A1

公开(公告)日：2021-06-10

申请号：US17181271

申请日：2021-02-22

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Eric Hans Vermaas ,  Mahdieh Khosroheidari ,  Claire Bevis-Mott

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6834 ,  C12Q1/6809 ,  C12Q1/6816 ,  C40B50/16 ,  C40B20/04 ,  C40B70/00 ,  C40B50/18 ,  C40B80/00

Abstract: The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by modifying or blocking 5′ and 3′ ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.

公开(公告)号：US11015266B2

公开(公告)日：2021-05-25

申请号：US16943630

申请日：2020-07-30

Applicant: City of Hope

Inventor： Jacob Berlin ,  Gregory Copeland ,  Kathleen Elison ,  Hurik Muradyan

IPC: C40B50/16 ,  G01N33/543 ,  C12N15/10 ,  C40B40/10 ,  C40B70/00 ,  B82Y5/00

Abstract: Provided herein are encoded split pool libraries useful, inter alia, for forming highly diverse and dense arrays for screening and detection of a variety of molecules.

公开(公告)号：US20200378031A1

公开(公告)日：2020-12-03

申请号：US16940499

申请日：2020-07-28

Applicant: City of Hope

Inventor： Jacob BERLIN ,  Gregory COPELAND ,  Kathleen ELISON ,  Hurik MURADYAN

IPC: C40B50/16 ,  G01N33/543 ,  C12N15/10 ,  C40B40/10 ,  C40B70/00

Abstract: Provided herein are encoded split pool libraries useful, inter alia, for forming highly diverse and dense arrays for screening and detection of a variety of molecules.

公开(公告)号：US20200347381A1

公开(公告)日：2020-11-05

申请号：US16934251

申请日：2020-07-21

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Francois Vigneault

IPC: C12N15/10 ,  C12Q1/6869 ,  C40B50/16 ,  C12Q1/6806

Abstract: Methods and compositions for high-throughput, single cell analyses are provided. The methods and compositions can be used for analysis of genomes and transcriptomes, as well as antibody discovery, HLA typing, haplotyping and drug discovery.

公开(公告)号：US10767278B2

公开(公告)日：2020-09-08

申请号：US16555531

申请日：2019-08-29

Applicant: City of Hope

Inventor： Jacob Berlin ,  Gregory Copeland ,  Kathleen Elison ,  Hurik Muradyan

IPC: C40B50/16 ,  G01N33/543 ,  C40B40/10 ,  C40B70/00 ,  C12N15/10 ,  B82Y5/00

Abstract: Provided herein are encoded split pool libraries useful, inter alia, for forming highly diverse and dense arrays for screening and detection of a variety of molecules.

公开(公告)号：US10752895B2

公开(公告)日：2020-08-25

申请号：US16270941

申请日：2019-02-08

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Francois Vigneault

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6869 ,  C40B50/16 ,  C12Q1/6806

Abstract: Methods and compositions for high-throughput, single cell analyses are provided. The methods and compositions can be used for analysis of genomes and transcriptomes, as well as antibody discovery, HLA typing, haplotyping and drug discovery.

公开(公告)号：US10246703B2

公开(公告)日：2019-04-02

申请号：US15874137

申请日：2018-01-18

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Francois Vigneault

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6869 ,  C40B50/16 ,  C12Q1/6806

Abstract: Methods and compositions for high-throughput, single cell analyzes are provided. The methods and compositions can be used for analysis of genomes and transcriptomes, as well as antibody discovery, HLA typing, haplotyping and drug discovery.

公开(公告)号：US09920084B2

公开(公告)日：2018-03-20

申请号：US14240067

申请日：2012-08-23

Applicant: Masad J. Damha ,  Matthew Hassler ,  Tak-Hang Chan ,  Mallikarjuna Reddy Nandyala ,  Robert Alexander Donga

Inventor： Masad J. Damha ,  Matthew Hassler ,  Tak-Hang Chan ,  Mallikarjuna Reddy Nandyala ,  Robert Alexander Donga

IPC: C07D233/60 ,  C07D333/00 ,  C40B80/00 ,  C07F9/54 ,  C07H19/02 ,  C07H21/00 ,  C07F9/655 ,  C07F9/6558 ,  C07H21/02 ,  C40B50/16

CPC classification number: C07F9/54 ,  C07D233/60 ,  C07F9/5407 ,  C07F9/5442 ,  C07F9/5456 ,  C07F9/65515 ,  C07F9/65586 ,  C07H19/02 ,  C07H21/00 ,  C07H21/02 ,  C40B50/16 ,  C40B80/00

Abstract: The invention relates to the chemical synthesis of oligonucleotides, e.g., oligoribonucleotides. In another aspect, the invention relates to compounds of formula (II) processes for making these compounds, and the use thereof in the chemical synthesis of oligonucleotides, e.g., oligoribonucleotides. The invention also relates to methods of synthesis of oligomers, including but not limited to oligopeptides, oligosaccharides and oligonucleotides, particularly oligoribonucleotides and also oligodeoxyribonucleotides, in solution systems, and ionic tag linkers for use in methods provided herein.

公开(公告)号：US09868986B2

公开(公告)日：2018-01-16

申请号：US14675981

申请日：2015-04-19

Applicant: BioInventors & Entrepreneurs Network, LLC

Inventor： Michael Seul

IPC: C12Q1/68 ,  C40B20/04 ,  C40B50/10 ,  C40B50/16 ,  G06Q30/00 ,  G06Q30/06 ,  G06Q50/22

CPC classification number: C12Q1/6874 ,  C12Q1/6827 ,  C40B20/04 ,  C40B50/10 ,  C40B50/16 ,  G06Q30/018 ,  G06Q30/0631 ,  G06Q50/22 ,  G06Q2220/10 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2563/179

Abstract: A method of selecting nucleic acid samples including particular desired alleles from a plurality of nucleic acid samples including the steps of performing a first reaction in a plurality of pools containing the samples to produce reaction products including a source tag identifying said each pool; pooling the pools to provide pooled pools; for each of the desired alleles to be identified, performing a second reaction using said reaction products to produce allele-specific second reaction products comprising a marker tag and a derived source tag; identifying said allele-specific second reaction products, and further identifying nucleic acid samples with the desired alleles. A source tag sharing number “d” may be determined for each of the alleles. Alleles may also be binned together.

公开(公告)号：US09487775B2

公开(公告)日：2016-11-08

申请号：US14861735

申请日：2015-09-22

Applicant: Nuevolution A/S

Inventor： Thomas Franch ,  Alex Haahr Gouliaev ,  Per-Ola Freskgard ,  Mikkel Dybro Lundorf ,  Jakob Felding ,  Eva Kampmann Olsen ,  Anette Holtmann ,  Soeren Nyboe Jakobsen ,  Christian Klarner Sams ,  Sanne Birkebaek Jensen ,  Kim Birkebaek Jensen ,  Henrik Pedersen

IPC: C12Q1/68 ,  C12N15/10 ,  C40B50/10 ,  C40B50/16

CPC classification number: C12N15/1065 ,  C12N15/1068 ,  C12Q1/6806 ,  C12Q2521/101 ,  C12Q2521/501 ,  C40B50/10 ,  C40B50/16

Abstract: Disclosed is a method for obtaining a bifunctional complex comprising a display molecule part and a coding part, wherein a nascent bifuntional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is reacted at the chemical reaction site with one or more reactants, and provided with respective tag(s) identifying the reactant(s) at the priming site is using one or more enzymes.