公开(公告)号：US20230220352A1

公开(公告)日：2023-07-13

申请号：US17776100

申请日：2020-11-12

Applicant: JUNTENDO EDUCATIONAL FOUNDATION

Inventor： Yasushi OKAZAKI ,  Masahito MATSUMOTO ,  Hiroko HAGIWARA

IPC: C12N5/071 ,  C12N5/077 ,  C12N5/0793 ,  C12N15/90

CPC classification number: C12N5/067 ,  C12N5/0653 ,  C12N5/0619 ,  C12N5/0654 ,  C12N5/0657 ,  C12N15/907 ,  C12N2500/02 ,  C12N2506/1353 ,  C12N2501/165 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/33 ,  C12N2501/12 ,  C12N2501/11

Abstract: A method of direct transdifferentiation of somatic cells into other somatic cells may be convenient and still have good reproducibility, excellent production efficiency, and short performed time. Methods for direct transdifferentiation of somatic cells into other somatic cells may include: (a) introducing a GLIS family gene, a mutated GLIS family gene or a gene product thereof into somatic cells; and (b) culturing the gene-introduced somatic cells in a culture medium containing a component that induces differentiation of the somatic cells or precursor cells of the somatic cells into other somatic cells.

公开(公告)号：US20230130038A1

公开(公告)日：2023-04-27

申请号：US17977788

申请日：2022-10-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Sanjay K. VASU ,  Chao Yan LIU ,  Jeffrey ROGERS ,  Maria Isabel CISNEROS ,  Jingqui LI ,  Henry C. CHIOU ,  Meredith Bauman JONES

IPC: C07K14/505 ,  C12N5/00 ,  C12N5/0793

Abstract: High-yield mammalian transient expression systems can include a cell culture media (particularly serum free, non-animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

公开(公告)号：US20230129433A1

公开(公告)日：2023-04-27

申请号：US18049327

申请日：2022-10-25

Applicant: Axogen Corporation

Inventor： Curt DEISTER ,  Kasra TAJDARAN

IPC: G01N1/30 ,  G01N33/483 ,  C12N5/0793

Abstract: Techniques and systems are disclosed for a bioassay that is an in vitro mimic of peripheral nerve generation using the sensory neurons that innervate the peripheral nervous system. In some embodiments, the techniques may assist in detecting the bioactivity or potency of nerve grafts (e.g., processed, acellular human allografts) for fostering or supporting peripheral nerve regeneration. In various embodiments, techniques comprise affixing neurons (e.g., a DRG) to a nerve graft segment to form a test construct; culturing the test construct in a medium; analyzing the test construct to indicate the amount of outgrowing nerve structure; and determining the potency of the nerve graft from a metric derived from the analysis. In some embodiments, techniques and materials may be used to test the effect of a varied test condition on nerve growth.

公开(公告)号：US20230090462A1

公开(公告)日：2023-03-23

申请号：US17439251

申请日：2020-03-16

Applicant: The Schepens Eye Research Institute, Inc. ,  Massachusetts Eye and Ear Infirmary ,  Massachusetts Institute of Technology

Inventor： Michael J. Young ,  Pierre Colombe ,  Deepti Singh

IPC: A61K35/545 ,  C12N5/0793 ,  A61P27/02 ,  A61K9/00 ,  A61K49/00

Abstract: Progenitor cells were isolated, purified and expanded using a microfluidic based cell sorting approach. The methods were successfully in purifying cone progenitor cells (CPCs) defined based on a proliferative population expressing cone arrestin and Red/Green (R/G) opsin at greater than 80% using a two multistage approach.

公开(公告)号：US20230090286A1

公开(公告)日：2023-03-23

申请号：US17929647

申请日：2022-09-02

Applicant: AKOUOS, INC.

Inventor： Emmanuel John Simons ,  Ellen Reisinger ,  Sebastian Kügler ,  Hanan Al-Moyed

IPC: C12N15/52 ,  A61K38/17 ,  A61K48/00 ,  A61P27/16 ,  C07K14/47 ,  C12N5/0793 ,  C12N9/16 ,  C12N15/65 ,  C12N15/86 ,  C12N15/90 ,  A01K67/027

Abstract: Provided herein are compositions that include at least two different nucleic acid vectors, where each of the at least two different vectors includes a coding sequence that encodes a different portion of an otoferlin protein, and the use of these compositions to treat hearing loss in a subject.

公开(公告)号：US11603518B2

公开(公告)日：2023-03-14

申请号：US16750975

申请日：2020-01-23

Applicant: Asterias Biotherapeutics, Inc.

Inventor： Kento Onishi ,  Nathan C. Manley ,  Craig R. Halberstadt ,  Erik M. Whiteley

IPC: C12N5/0793

Abstract: Methods for differentiating human pluripotent stem cells to dorsal neuroectoderm progenitors and further to glial progenitor cells and oligodendrocyte progenitor cells (OPCs) using inhibitors of BMP signaling and MAPK/ERK signaling are provided. Also provided are cells and cellular compositions obtained by such methods, and uses of such cells. Further provided are methods and protocols for efficiently differentiating human pluripotent stem cells to OPCs in the absence of the ventralizing morphogen SHH or a SHH signaling activator. The methods of the present disclosure reproducibly produce dorsal neuroectoderm progenitor cells by day 7 of the differentiation process, glial progenitor cells by day 21 of the differentiation process and OPCs by day 42 of the differentiation process.

公开(公告)号：US11591567B2

公开(公告)日：2023-02-28

申请号：US16373026

申请日：2019-04-02

Applicant: MEMORIAL SLOAN-KETTERING CANCER CENTER

Inventor： Stuart Chambers ,  Lorenz Studer ,  Zehra Dincer ,  Bastian Zimmer

IPC: C12N5/0793 ,  A61K35/12 ,  C07D211/00 ,  C07D307/32 ,  C07D243/36

Abstract: Cranial placodes are embryonic structures essential for sensory and endocrine organ development. The efficient derivation of cranial placodes from human pluripotent stem cells is disclosed where the timed removal of the BMP inhibitor Noggin, a component of the dual-SMAD inhibition strategy of neural induction, triggers placode induction at the expense of CNS fates. Further fate specification at the pre-placode stage enables the selective generation of placode-derived trigeminal ganglia capable of in vivo engraftment, mature lens fibers and anterior pituitary hormone-producing cells that upon transplantation produce hormones including, but not limited to, human growth hormone and adrenocortiocotropic hormone in vivo. Alternatively, anterior pituitary hormone-producing cells are generated in cell culture systems in vitro.

公开(公告)号：US20230049017A1

公开(公告)日：2023-02-16

申请号：US17758696

申请日：2020-01-13

Applicant: CORESTEM CO., LTD

Inventor： Sang Hun LEE ,  Mi Yoon CHANG ,  Seung Won KIM ,  Hye Ji WOO ,  Yanuar Alan SULISTIO

IPC: A61K35/30 ,  C12N5/0793 ,  C12N5/079 ,  G01N33/50 ,  A61P25/28

Abstract: The present invention relates to a method of patterning and chopping 3D organoids prepared from human pluripotent stem cells, culturing the stem cells or progenitor cells, and inducing the differentiation thereof to obtain a large amount of finally differentiated cells. Compared to cells differentiated by a conventional differentiation method, the cells obtained in a large amount exhibit remarkably superior effects in terms of reproducibility, stability, and functionality, and thus are expected to be very useful for cell therapeutic agents or for the screening of therapeutic drugs.

公开(公告)号：US11543404B2

公开(公告)日：2023-01-03

申请号：US16763944

申请日：2018-11-15

Applicant: Stem Cell & Device Laboratory, Inc.

Inventor： Kazuhiro Aiba ,  Norie Tooi ,  Kosuke Hori ,  Masaki Sudo

IPC: G01N33/50 ,  C12N11/082 ,  C12N11/089 ,  C12N5/0793 ,  C12N11/096

Abstract: The present invention provides a nerve cell device in which early observation of nerve activity (spikes, bursts, and the like) is made possible and the measured electric strength is increased by cultivating neurons upon a cell scaffold. By using this nerve cell device, imaging of intracellular signaling is also possible.

公开(公告)号：US20220364058A1

公开(公告)日：2022-11-17

申请号：US17844757

申请日：2022-06-21

Applicant: KEIO UNIVERSITY

Inventor： Minoru Ko

IPC: C12N5/077 ,  C12N15/09 ,  C12N5/0793 ,  C12N5/071 ,  C12N15/113

Abstract: In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being highly efficient. Many attempts have been made, including a stepwise differentiation induction method based on the control of culture conditions or the addition of, for example, various cell growth factors/differentiation factors to a culture solution, but the use of complicated culture steps is a big problem. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by use of a pluripotent stem cell that actively undergoes cell differentiation, which is obtained by reducing an undifferentiated state of the pluripotent stem cell, has been developed, and thus the present invention has been completed.