公开(公告)号：US20050191677A1

公开(公告)日：2005-09-01

申请号：US11054621

申请日：2005-02-09

Applicant: Jochen Franzen ,  Detlev Suckau

Inventor： Jochen Franzen ,  Detlev Suckau

IPC: G01N33/543 ,  G01N33/68 ,  C12Q1/68 ,  G01N33/553

CPC classification number: G01N33/6848 ,  G01N33/54326

Abstract: The invention relates to the determination of the relative concentrations of proteins or protein derivatives in liquids. The invention provides a method which uses nanoparticles coated with specific affinity collectors in order to fish the desired proteins or protein derivatives out of the liquids and to separate them, in order to introduce them to the mass spectrometric frequency analysis after elution from the affinity collectors. This makes it possible to determine the concentrations of several proteins or several forms of protein modification or mutation relative to each other with relatively high measuring dynamics.

Abstract translation: 本发明涉及测定液体中蛋白质或蛋白质衍生物的相对浓度。 本发明提供了一种方法，其使用涂覆有特异性亲和收集剂的纳米颗粒，以从液体中捕获所需的蛋白质或蛋白质衍生物并将其分离，以便将它们从亲和收集剂洗脱后引入质谱分析。 这使得可以通过相对较高的测量动力学确定几种蛋白质的浓度或相对于彼此的几种形式的蛋白质修饰或突变。

公开(公告)号：US20050023461A1

公开(公告)日：2005-02-03

申请号：US10849499

申请日：2004-05-19

Applicant: Michael Schubert ,  Jochen Franzen

Inventor： Michael Schubert ,  Jochen Franzen

IPC: G01N23/00 ,  H01J49/42

CPC classification number: H01J49/424 ,  H01J49/067 ,  H01J49/4295

Abstract: The invention relates to methods and devices for the effective capturing of externally generated ions in an RF operated quadrupole ion trap. The invention involves applying a voltage consisting of positive and negative pulses, instead of a sinusoidal RF voltage, during the capturing process, with capturing intervals between each pulse in which the voltage is low.

Abstract translation: 本发明涉及用于在RF操作的四极离子阱中有效捕获外部产生的离子的方法和装置。 本发明涉及在捕获过程期间施加由正脉冲和负脉冲组成的电压而不是正弦RF电压，其中电压低的每个脉冲之间的捕获间隔。

公开(公告)号：US06770443B2

公开(公告)日：2004-08-03

申请号：US10099157

申请日：2002-03-15

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: C12Q168

CPC classification number: H01J49/04 ,  C12Q1/6827 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2565/501 ,  C12Q2523/319

Abstract: Methods and equipment are provided for the mass spectrometric measurement of a large number of genotyping profiles, each formed by several tens to several hundreds of SNPs (single nucleotide polymorphisms). A multitude of chips each carrying an array of surface-bound oligonucleotide probes for mutations are synchronously processed. The chips are attached to plates such that they can be immersed in a multitude of wells with DNA samples requiring analysis while also serving directly as sample carriers in mass spectrometers. The multitude of wells can, for instance, take the form of microtitre plates. Primers may be used which possess a photolytically or chemically cleavable linker that bridges one base pair and does not hinder either the possibility of hybridization or enzymatic extension. Light or chemicals can then be used to cleave short chains particularly suitable for ionization by matrix assisted laser desorption and mass spectrometric analysis using time-of-flight mass spectrometers.

Abstract translation: 提供了方法和设备用于大量基因分型谱的质谱测量，每个基因型分布由几十到几百个SNP（单核苷酸多态性）形成。 同时处理各自携带用于突变的表面结合寡核苷酸探针阵列的多个芯片。 芯片连接到板上，使得它们可以浸入需要分析的DNA样品的多个孔中，同时也可以直接作为质谱仪中的样品载体。 例如，许多井可采取微量滴定板的形式。 可以使用引物，其具有桥接一个碱基对的光解或化学可切割的接头，并且不妨碍杂交或酶促延伸的可能性。 然后可以使用光或化学品来切割特别适合离子化的短链，通过使用飞行时间质谱仪的基质辅助激光解吸和质谱分析。

公开(公告)号：US06717132B2

公开(公告)日：2004-04-06

申请号：US09778654

申请日：2001-02-07

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: B01D5944

CPC classification number: H01J49/401 ,  H01J49/06 ,  H01J49/405

Abstract: The invention relates to a time-of-flight mass spectrometer for injection of the ions orthogonally to the time-resolving axis-of-flight component, with a pulser for acceleration of the ions of the beam in the axis-of-flight direction, preferably with a velocity-focusing reflector for reflecting the ion beam and with a flat detector at the end of the flight section. The invention consists of using, both for acceleration in the pulser and for reflection in the reflectors, a gridless optical system made up of slit diaphragms which can spatially focus the ions onto the detector in the direction vertical to the directions of injection and flight axis, but which does not have any focusing or deflecting effect on the other directions. For some reflector geometries it is essential to use an additional cylindrical lens for focusing, and for other reflector geometries the use of such a lens may be advantageous.

Abstract translation: 本发明涉及一种飞行时间质谱仪，用于通过脉冲发生器在垂直于飞行时间的方向加速离子的离子注入离子与时间分辨的飞行时间分量成正比， 优选地具有用于反射离子束的速度聚焦反射器和在飞行部分的末端处的平坦检测器。 本发明包括使用脉冲发生器加速和反射器反射的无栅光学系统，其由狭缝光阑构成，其可以在垂直于注射和飞行轴线的方向上将离子空间聚焦到检测器上， 但是对其他方向没有任何聚焦或偏转效应。 对于一些反射器几何，必须使用附加的柱面透镜进行聚焦，并且对于其它反射器几何形状，使用这种透镜可能是有利的。

公开(公告)号：US06624408B1

公开(公告)日：2003-09-23

申请号：US09407729

申请日：1999-09-28

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: B01D5944

CPC classification number: H01J49/0036

Abstract: The invention relates to the scanning and representation of daughter ion spectra for the purpose of determining the structural characteristics of parent ions in ion traps. The invention consists of combining all or selected daughter and granddaughter spectra of a parent ion over several generations in one combined descendants spectrum. This combined descendants spectrum can be depicted as a graphic or a list. The references to origin can be plotted on the combined descendants spectrum. For biopolymers, where the loss of fragments can be identified due to their mass, the names or abbreviations of lost molecule fragments can be entered. The criteria for selection of the spectra can be predefined; in this way, the spectra can be depicted and even scanned automatically.

Abstract translation: 本发明涉及扫描和表示子离子谱，以确定离子阱中母体离子的结构特征。 本发明包括在一个组合的后代谱中结合几代的母体离子的所有或选定的女儿和孙女光谱。 这种组合的后代谱可以被描绘成图形或列表。 对起源的引用可以绘制在组合的后代谱上。 对于生物聚合物，其中由于其质量可以鉴定片段的损失，可以输入丢失的分子片段的名称或缩写。 选择光谱的标准可以预先定义; 以这种方式，可以自动绘制和均匀扫描光谱。

公开(公告)号：US06555814B1

公开(公告)日：2003-04-29

申请号：US09607774

申请日：2000-06-30

Applicant: Gökhan Baykut ,  Jochen Franzen

Inventor： Gökhan Baykut ,  Jochen Franzen

IPC: B01D5944

CPC classification number: H01J49/063 ,  H01J49/38 ,  H01J49/4265

Abstract: The invention relates to a method and a device for controlling the number of ions in ion cyclotron resonance (ICR) mass spectrometers, whereby the ions enter a multipole ion guide after their formation and are stored there temporarily. By measuring the ion number in a predefined subset of these temporarily stored ions, the number of ions transferred into the ICR trap for mass spectrometric analysis is regulated. A mode of operation of the multipole ion guide can ensure that undesirable mass ranges are filtered out before the transfer of ions into the ICR mass spectrometer. The invention makes it possible to eliminate space charge effects, which are caused by overfilling the ICR traps.

Abstract translation: 本发明涉及一种用于控制离子回旋共振（ICR）质谱仪中的离子数的方法和装置，由此离子在其形成后进入多极离子导向器并暂时储存在其中。 通过测量这些临时存储的离子的预定子集中的离子数，调节转移到用于质谱分析的ICR阱中的离子数。 多极离子导向器的操作模式可以确保在将离子转移到ICR质谱仪之前过滤不需要的质量范围。 本发明使得可以消除由过度填充ICR陷阱引起的空间电荷效应。

公开(公告)号：US06180372B2

公开(公告)日：2001-01-30

申请号：US09049646

申请日：1998-03-27

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: C12P1934

CPC classification number: B01L3/502707 ,  B01L7/52 ,  B01L7/525 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0487 ,  B01L2400/084 ,  C12Q1/686 ,  Y10S977/775 ,  Y10S977/783 ,  Y10S977/788 ,  Y10S977/80 ,  Y10S977/924 ,  C12Q2565/629 ,  C12Q2527/125 ,  C12Q2527/113

Abstract: The invention concerns methods and instruments for fast, selective replication of deoxyribonucleic acid (DNA) from biomaterial through the known polymerase chain reaction (PCR), working in individual duplication thermocycles. The invention consists of extremely brief cycle times of only a few seconds for the PCR reactions, generated, on the one hand, by reaction chambers for the reception of the reaction solution constructed of a pattern of fine capillaries in close proximity to heating and cooling elements in order to optimally accelerate the temperature setting in the reaction solution for the three temperature phases of the PCR duplication cycles and, on the other hand, by keeping the flow rates in the capillaries to a minimum during the amplification phase so that the polymerase reaction is not disturbed. The capillary pattern can be simply produced by means of microsystern technology.

Abstract translation: 本发明涉及通过已知的聚合酶链式反应（PCR）从生物材料快速，选择性复制脱氧核糖核酸（DNA）的方法和工具，在单独的复制热循环中工作。 本发明由PCR反应的极短的循环时间仅为几秒钟，一方面由反应室产生，用于接收由加热和冷却元件附近的精细毛细管图案构成的反应溶液 为了最佳地加速PCR复制循环的三个温度阶段的反应溶液中的温度设定，另一方面，通过在扩增阶段将毛细管中的流速保持在最小值，使得聚合酶反应为 不打扰 毛细管图案可以通过微观技术简单地生产。

公开(公告)号：US6049077A

公开(公告)日：2000-04-11

申请号：US133868

申请日：1998-08-13

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: H01J49/40

CPC classification number: H01J49/40

Abstract: The time-of-flight mass spectrometers which must demonstrate a high constancy of the calibrated mass scale even under changeable ambient temperatures and thermal loads due to pumps or electronics. Time-of-flight mass spectrometers calculate the masses of ions from the measured time of flight in a long flight tube that is normally manufactured of stainless steel. These flight tubes are subject to temperature-related length changes which affect the flight time and therefore the mass determination. The thermal expansion of spectrometer parts between ion source and ion detector, thus keeping the flight path for the ions at a constant length. Length compensation can be produced by design of the spacing system made of materials of different thermal expansion coefficients, the length changes of which balance out in opposite directions.

Abstract translation: 飞行时间质谱仪即使在可变环境温度和由于泵或电子设备造成的热负载的情况下也必须表现出校准质量标度的高恒定性。 飞行时间质谱仪从通常由不锈钢制造的长飞行管中测量的飞行时间计算离子质量。 这些飞行管受到温度相关的长度变化，影响飞行时间，因此影响质量测定。 离子源和离子检测器之间的光谱仪部分的热膨胀，从而保持离子的飞行路径恒定的长度。 长度补偿可以通过设计由不同热膨胀系数的材料制成的间隔系统，其长度变化在相反方向平衡。

公开(公告)号：US5969348A

公开(公告)日：1999-10-19

申请号：US933862

申请日：1997-09-19

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: H01J49/40 ,  B01D59/44 ,  H01J49/00

CPC classification number: H01J49/403

Abstract: The invention relates to measurement methods for time-of-flight mass spectrometers which operate with an ionization of analyte substances adsorbed at the surface of a sample support and an improvement in mass resolution through delayed ion acceleration (or "delayed extraction") in front of the sample support. It particularly relates to velocity focusing for good mass resolution simultaneously for wide ranges of masses within the spectrum.The invention consists of focusing the flight-times of the ions simultaneously for all masses in wide ranges of interest relative to their initial velocity, by allowing the acceleration in the first accelerating region to increase in time after being switched on. Thus a good resolution cannot only be set for one mass on the spectrum but for all masses in wide ranges simultaneously. In computer simulations, provided there is a correlation of space and velocity distribution, focusing of a least first order is obtained simultaneously for all ions.

Abstract translation: 本发明涉及飞行时间质谱仪的测量方法，其通过吸附在样品载体表面的分析物质的离子化和通过延迟离子加速（或“延迟提取”）提高质量分辨率而工作， 样品支持。 它特别涉及速度聚焦，用于在光谱范围内的宽范围的质量范围内同时获得良好的质量分辨率。 本发明包括通过允许第一加速区域中的加速度在接通之后随时间增加，将离子的飞行时间同时聚焦在相对于其初始速度的广泛范围内的所有质量。 因此，不仅可以在光谱上设置一个质量，而且可以同时为宽范围内的所有质量设置好的分辨率。 在计算机模拟中，只要存在空间和速度分布的相关性，所有离子同时获得最小一阶的聚焦。

公开(公告)号：US5936241A

公开(公告)日：1999-08-10

申请号：US32579

申请日：1998-02-27

Applicant: Jochen Franzen ,  Michael Schubert

Inventor： Jochen Franzen ,  Michael Schubert

IPC: H01J49/42

CPC classification number: H01J49/4265

Abstract: The invention consists of deriving the control of the space charge in the ion trap for the initial daughter ion spectrum from the filling rates of previous normal spectra, from the abundance ratio of the parent ions to be isolated to the total ions in the spectrum, and from the at least roughly known isolation and fragmentation yields. For further daughter ion spectra, the resulting measured overall filling rate with daughter ions may be used. The same applies in an analogue way to spectra of isolated ions or of ions from MS.sup.n processes.

Abstract translation: 本发明包括从先前正常光谱的填充率，从要分离的母离子的丰度比与光谱中的总离子的丰度比，导出离子阱中离子阱中空间电荷的控制，以及 从至少大致已知的分离和碎裂产率。 对于更多的子离子光谱，可以使用与子离子的测得的总体填充率。 同样的情况也适用于分离离子或MSn过程离子的光谱。