公开(公告)号：US20060005273A1

公开(公告)日：2006-01-05

申请号：US11148754

申请日：2005-06-09

Applicant: Sairam Rudrabhatla ,  Stephen Goldman ,  Diaa Al- Abed

Inventor： Sairam Rudrabhatla ,  Stephen Goldman ,  Diaa Al- Abed

IPC: A01H1/00 ,  C12N15/82 ,  A01H5/00

CPC classification number: A01H4/00 ,  A01H4/001 ,  A01H4/005 ,  A01H4/008 ,  C12N5/0025 ,  C12N15/8201

Abstract: The present invention provides an efficient and novel maize transformation and regeneration system based on a novel split-seed explant. Mature maize seeds are split longitudinally to form a split-seed explant. The split-seed explant can then be used in transformations to introduce a gene of interest into the maize genome to produce novel maize lines having desired characteristics. The split-seed explant can also be used to generate calli and/or multiple shoots, and rooted plantlets.

Abstract translation: 本发明提供了一种基于新颖的分裂种子外植体的有效和新颖的玉米转化和再生系统。 成熟的玉米种子纵向分裂以形成裂殖种子外植体。 然后分裂种子外植体可用于转化以将目的基因引入玉米基因组以产生具有所需特征的新型玉米品系。 分裂种子外植体也可用于产生愈伤组织和/或多个芽，以及根植物。

公开(公告)号：US20050246955A1

公开(公告)日：2005-11-10

申请号：US11111553

申请日：2005-04-21

Applicant: W. Bissonnette ,  Robert Wainwright ,  John Thompson ,  Carson Payne ,  Sylvia Bernstein ,  Curt Morgan ,  Robert Bromley ,  Andrew Bridgeman ,  Laura Conley ,  Ann Forsthoefel ,  Robert Showalter ,  Frederic Wiedemann

Inventor： W. Bissonnette ,  Robert Wainwright ,  John Thompson ,  Carson Payne ,  Sylvia Bernstein ,  Curt Morgan ,  Robert Bromley ,  Andrew Bridgeman ,  Laura Conley ,  Ann Forsthoefel ,  Robert Showalter ,  Frederic Wiedemann

IPC: A01C1/02 ,  A01G31/00 ,  A01G31/02 ,  A01H4/00

CPC classification number: A01C1/02 ,  A01G31/02 ,  A01H4/001 ,  Y02P60/216

Abstract: This invention provides methods, devices, and kits for growing a plant or germinating a seed into a plant; methods and devices for delivering oxygen to a plant or a seed which will germinate into a plant; methods and devices for delivering liquid to a plant; methods and devices for increasing the dissolved oxygen concentration in a liquid to be delivered to a plant; methods and devices for increasing the dissolved oxygen concentration in a liquid within a hydroponics device; terraced oxygenators; aspirators, downdraft venturis, net baskets; germination caps, sets of germination caps; methods for increasing the likelihood of germination of a seed; seed-bearing support media; methods for germinating a seed; and smart garden devices for hydroponics growing systems.

公开(公告)号：US20040259831A1

公开(公告)日：2004-12-23

申请号：US10891258

申请日：2004-07-14

Inventor： Bruce Marvin Held ,  Herbert Martin Wilson ,  Liming Hou ,  Carol Jean Lewnau ,  Janelle Christine Eby

IPC: A01N043/04 ,  A01N063/00 ,  C12N015/82

CPC classification number: C12N15/895 ,  A01H4/001 ,  A01H4/005 ,  C12M35/02 ,  C12N5/0025 ,  C12N15/64 ,  C12N15/8201 ,  C12N15/8206 ,  C12N15/8207

Abstract: The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus production of elite lines of soybean.

Abstract translation: 本发明涉及在细胞和组织中引入包括核酸，碳水化合物，植物生长调节剂和肽的分子。 本发明还涉及用于增强大豆精英品系的胚发生愈伤组织生产的培养基和方法。

公开(公告)号：US20040259830A1

公开(公告)日：2004-12-23

申请号：US10891255

申请日：2004-07-14

Inventor： Bruce Marvin Held ,  Herbert Martin Wilson ,  Liming Hou ,  Carol Jean Lewnau ,  Janelle Christine Eby

IPC: A01N043/04 ,  A01N063/00 ,  C12N015/82 ,  A61K048/00

CPC classification number: C12N15/895 ,  A01H4/001 ,  A01H4/005 ,  C12M35/02 ,  C12N5/0025 ,  C12N15/64 ,  C12N15/8201 ,  C12N15/8206 ,  C12N15/8207

Abstract: The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus production of elite lines of soybean.

公开(公告)号：US06815205B2

公开(公告)日：2004-11-09

申请号：US09778132

申请日：2001-02-06

Applicant: Jhy-Jhu Lin ,  Jianqing Lan ,  Nacyra Assad-Garcia

Inventor： Jhy-Jhu Lin ,  Jianqing Lan ,  Nacyra Assad-Garcia

IPC: C12N500

CPC classification number: A01H4/001 ,  A01H4/005 ,  A01H4/008 ,  A01N43/38 ,  C07D209/18 ,  C12N5/0025 ,  C12N15/8201 ,  C12N15/8205

Abstract: The present invention provides compounds and compositions capable of stimulating plant growth, regeneration of plant cells and tissues, and transformation of plant cells and tissues, comprising mono- and multi-substituted auxinic analogues of indole-3-acetic acid (IAA) comprising substituent groups such as halo-, alkyl-, alkoxy-, acyl-, acylamido- and acyloxy-groups. The invention relates to a method of using such mono- and multi-substituted auxinic analogues of IAA to affect growth, regeneration or transformation in monocotyledonous and dicotyledonous plants, as well as in transgenic plant tissues. The invention also contemplates the use of these auxinic IAA analogues in the presence of other plant growth regulators, such as cytokinin, etc., to enhance plant growth.

公开(公告)号：US06723894B2

公开(公告)日：2004-04-20

申请号：US10047206

申请日：2002-01-15

Applicant: Anthony John Fist ,  Christopher James Byrne ,  Wayne Lyle Gerlach

Inventor： Anthony John Fist ,  Christopher James Byrne ,  Wayne Lyle Gerlach

IPC: C12P1716

CPC classification number: A01H5/04 ,  A01H4/001 ,  C07D489/02 ,  C07D489/08 ,  C07D489/12

Abstract: There is disclosed an improved poppy straw of a stably reproducing Papaver somniferum for the extraction of thebaine and/or oripavine, the threshed straw having thebaine and oripavine constituting about 50% by weight or greater of the alkaloid combination consisting of morphine, codeine, thebaine and oripavine.

公开(公告)号：US20030194281A1

公开(公告)日：2003-10-16

申请号：US10435339

申请日：2003-05-08

Applicant: Weyerhaeuser Company

Inventor： Michael K. McKinnis

IPC: B65G051/18

CPC classification number: A01H4/001 ,  B07C5/362 ,  Y10T137/87804

Abstract: An apparatus for directing objects carried serially by a fluid to a desired designation is provided. The apparatus includes an upstream fluid conduit and first and second downstream conduits. The apparatus also includes a sensor associated with the upstream fluid conduit operable to provide information regarding an object at a location in the upstream conduit. A switch is coupled to the upstream fluid conduit and selectively operable to deliver the object from the upstream fluid conduit to either the first or second downstream fluid conduit according to the information by applying a force to at least one of the upstream or downstream fluid conduits.

Abstract translation: 提供了一种用于将由流体串联运送的物体引导到所需指定的装置。 该装置包括上游流体导管和第一和第二下游导管。 该设备还包括与上游流体管道相关联的传感器，其可操作以提供关于上游管道中的位置处的物体的信息。 开关联接到上游流体管道，并且可选择性地操作以根据该信息将物体从上游流体导管输送到第一或第二下游流体导管，通过向至少一个上游流体管道或下游流体管道施加力。

公开(公告)号：US20030143744A1

公开(公告)日：2003-07-31

申请号：US10220837

申请日：2003-01-13

Inventor： James Martin Dunwell ,  Deborah Jane Keith ,  Jose Manso Preto Nobre

IPC: C12N005/04 ,  C12N015/82

CPC classification number: C12N15/8201 ,  A01H4/001 ,  A01H4/005 ,  A01H4/008

Abstract: The present invention provides Inter alia, a method for the production of cotton somatic embryos comprising (a) isolating a totipotent stomatal cell-containing epidermal explant from leaf material excised from a cotton plant; and (b) culturing said explant in a basal medium which comprises an embryogenic callus-inducing quantity of an auxin and a cytokinin under an embryogenic callus inducing intensity of light until embryogenic callus is formed; and (c) sub-culturing said embryogenic callus onto a somatic embryo differentiation media to produce said somatic embryos. Plants may be regenerated from the somatic embryos and in a particular embodiment of the invention said totipotent stomatal cell is transformed, prior to the inducement of embryogenic callus, with a polynucleotide that provides for a desired agronomic trait.

Abstract translation: 本发明尤其提供了一种用于生产棉花体细胞胚的方法，其包括（a）从棉花植物切除的叶材料中分离具有全能气孔细胞的表皮外植体; 和（b）在基础培养基中培养所述外植体，所述基础培养基包含胚胎发生愈伤组织诱导量的生长素和细胞分裂素，在胚发生愈伤组织下诱导强度直到形成胚性愈伤组织; 和（c）将所述胚性愈伤组织亚细培养到体细胞胚分化培养基上以产生所述体细胞胚。 植物可以从体细胞胚再生，并且在本发明的特定实施方案中，在诱导胚性愈伤组织之前，用提供所需农艺性状的多核苷酸转化全能气孔细胞。

公开(公告)号：US06569680B2

公开(公告)日：2003-05-27

申请号：US09820556

申请日：2001-03-29

Applicant: Pratap Kumar Pati ,  Madhu Sharma ,  Paramvir Singh Ahuja

Inventor： Pratap Kumar Pati ,  Madhu Sharma ,  Paramvir Singh Ahuja

IPC: C12N502

CPC classification number: A01H4/005 ,  A01H4/001 ,  C12N5/0025 ,  C12N5/04

Abstract: The invention a novel and efficient method for protoplast culture comprising the steps of isolating the protoplasts from a cell suspension, mixing the protoplasts with equal volumes of alginate solution, placing 40-50 &mgr;l of CaCl2 solution on a glass microslide, placing a mixture of protoplasts and alginate solution on the glass microslide and immediately covering by a glass coverglass, adding CaCl2 solution from the sides of coverglass, sliding down the coverglass towards one side and placing it in a petridish containing protoplast culture medium, sealing the petridishes, incubating it and transferring the extra thin alginate layer with celled colonies to regeneration medium for development of culture.

公开(公告)号：US20030074686A1

公开(公告)日：2003-04-17

申请号：US10053266

申请日：2002-01-17

Inventor： Ernst Heinz ,  Jodi Scheffler ,  Hjordis Voss

IPC: C12N015/82 ,  C12N015/87

CPC classification number: A01H5/10 ,  A01H4/001

Abstract: Provided is a method for the generation and selection of transgenic plant cells and tissue as well as plants of the genus Linum, in particular flax. Furthermore, transgenic plant cells, tissue and plants obtained by a method of the invention and their use in plant cell and tissue culture and plant breeding is described.

Abstract translation: 提供了用于产生和选择转基因植物细胞和组织以及Linum属植物，特别是亚麻的方法。 此外，描述了通过本发明的方法获得的转基因植物细胞，组织和植物及其在植物细胞和组织培养和植物育种中的用途。