公开(公告)号：US09399787B2

公开(公告)日：2016-07-26

申请号：US13720124

申请日：2012-12-19

Applicant: ACEA BIOSCIENCES, INC

Inventor： Yama A. Abassi ,  Xiaobo Wang ,  Xiao Xu

IPC: G01R27/08 ,  C12Q1/00 ,  C12Q1/70 ,  C12Q1/02 ,  G01N27/02 ,  G01N33/50 ,  G01N33/543 ,  G01N33/483

CPC classification number: C12Q1/025 ,  C12Q1/002 ,  G01N27/02 ,  G01N33/4836 ,  G01N33/5014 ,  G01N33/5438

Abstract: A method of performing an assay of a response of two or more cell types to a test compound, including: providing a device for monitoring cell-substrate impedance, adding at least two different cell types to the device; adding at least one test compound to form at least two test compound wells; providing at least two control wells; monitoring impedance of the at least two test compound wells and of the at least two control wells at three or more time points after adding the at least one test compound; and analyzing measured impedance from the at least two test compound wells and from the at least two control wells at the three or more time points to obtain information on the response of the different cell types to the at least one test compound.

Abstract translation: 一种对测试化合物进行两种或多种细胞类型的反应测定的方法，包括：提供用于监测细胞 - 基底阻抗的装置，向装置添加至少两种不同的细胞类型; 加入至少一种测试化合物以形成至少两个测试化合物孔; 提供至少两个对照孔; 在加入至少一种测试化合物之后，在三个或更多个时间点监测所述至少两个测试化合物孔和所述至少两个对照孔的阻抗; 以及在所述三个或更多个时间点分析来自所述至少两个测试化合物孔和所述至少两个对照孔的测量阻抗，以获得关于所述不同细胞类型对所述至少一种测试化合物的响应的信息。

公开(公告)号：US20150185206A1

公开(公告)日：2015-07-02

申请号：US14541024

申请日：2014-11-13

Applicant: ACEA Biosciences, Inc.

Inventor： Yama A. Abassi ,  Naichen Yu ,  Josephine Atienza ,  Xiao Xu ,  Xiaobo Wang

IPC: G01N33/50 ,  G01N27/02

CPC classification number: G01N33/5041 ,  C12Q1/002 ,  G01N27/021 ,  G01N33/5014 ,  G01N33/5438 ,  G01N2333/4716 ,  G01N2333/705 ,  G01N2333/91205 ,  G01N2500/04 ,  G01N2500/10

Abstract: Use of cell-substrate impedance based methods for screening for agonists of G-Protein Coupled Receptors (GPCRs) or inhibitors of a Receptor Tyrosine Kinases (RTKs), identifying compounds that affect GPCR or RTK pathways, validating molecular targets involved in a GPCR or RTK signaling pathways, monitoring dose-dependent functional activation of GPCR or RTK; determining desensitization of a GPCR and identifying a compound capable of affecting RTK activity in cancer cell proliferation.

Abstract translation: 使用基于细胞底物阻抗的方法筛选G-蛋白偶联受体（GPCR）的激动剂或受体酪氨酸激酶（RTK）的抑制剂，鉴定影响GPCR或RTK途径的化合物，验证参与GPCR或RTK的分子靶标 信号通路，监测GPCR或RTK的剂量依赖性功能活化; 测定GPCR的脱敏作用并鉴定能够影响癌细胞增殖中的RTK活性的化合物。

公开(公告)号：US20130123136A1

公开(公告)日：2013-05-16

申请号：US13720124

申请日：2012-12-19

Applicant: ACEA BIOSCIENCES, INC

Inventor： Yama A. Abassi ,  Xiaobo Wang ,  Xiao Xu

IPC: C12Q1/02

CPC classification number: C12Q1/025 ,  C12Q1/002 ,  G01N27/02 ,  G01N33/4836 ,  G01N33/5014 ,  G01N33/5438

Abstract: A method of performing an assay of a response of two or more cell types to a test compound, including: providing a device for monitoring cell-substrate impedance, adding at least two different cell types to the device; adding at least one test compound to form at least two test compound wells; providing at least two control wells; monitoring impedance of the at least two test compound wells and of the at least two control wells at three or more time points after adding the at least one test compound; and analyzing measured impedance from the at least two test compound wells and from the at least two control wells at the three or more time points to obtain information on the response of the different cell types to the at least one test compound.

Abstract translation: 一种对测试化合物进行两种或多种细胞类型的反应测定的方法，包括：提供用于监测细胞 - 基底阻抗的装置，向装置添加至少两种不同的细胞类型; 加入至少一种测试化合物以形成至少两个测试化合物孔; 提供至少两个对照孔; 在加入至少一种测试化合物之后，在三个或更多个时间点监测所述至少两个测试化合物孔和所述至少两个对照孔的阻抗; 以及在所述三个或更多个时间点分析来自所述至少两个测试化合物孔和所述至少两个对照孔的测量阻抗，以获得关于所述不同细胞类型对所述至少一种测试化合物的响应的信息。

公开(公告)号：US10799504B2

公开(公告)日：2020-10-13

申请号：US13740182

申请日：2013-01-12

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A61K31/506 ,  C07D401/14 ,  C07D403/12 ,  A61K45/06 ,  C07D239/42 ,  C07D401/12 ,  A61K31/519 ,  A61K31/52 ,  C07D473/32 ,  C07D487/04 ,  C07D473/34 ,  C07D239/48 ,  C07D473/24 ,  C07D239/46 ,  C07D473/40 ,  C07D487/02 ,  C07D473/16 ,  C07D239/47

Abstract: The present disclosure provides pharmaceutical compounds, compositions and methods, especially as they are related to compositions and methods for the treatment of tumors and related diseases related to the dysregulation of kinase (such as EGFR (including HER), Alk, PDGFR, but not limited to) pathways.

公开(公告)号：US10725023B2

公开(公告)日：2020-07-28

申请号：US16237382

申请日：2018-12-31

Applicant: ACEA Biosciences, Inc.

Inventor： Yama A. Abassi ,  Naichen Yu ,  Josephine Atienza ,  Xiao Xu ,  Xiaobo Wang

IPC: G01N33/50 ,  G01N33/543 ,  G01N27/02 ,  C12Q1/00

Abstract: A method of identifying a therapeutic compound for treating cancer in a human subject, the method including: providing a device that measures cell-substrate impedance; culturing cancer cells in the at least two wells, wherein the cancer cells are obtained from a human subject and have a receptor tyrosine kinase (RTK) pathway; adding to a first well a proposed therapeutic compound that affects an RTK pathway and an RTK stimulating factor for the RTK pathway to form a test well, and adding to another well the RTK stimulating factor to form a control well; continuously monitoring cell-substrate impedance of the at least two wells; and determining a difference in impedance or optionally in cell index between the test well and control well; and if significantly different, concluding the proposed therapeutic compound is therapeutically active in the RTK pathway within the cancer cells of the human subject.

公开(公告)号：US10596174B2

公开(公告)日：2020-03-24

申请号：US15435722

申请日：2017-02-17

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A61K31/519 ,  C07D487/04 ,  A61K31/52 ,  A61K31/506 ,  C07D473/32 ,  C07D401/14 ,  C07D473/34 ,  C07D403/12 ,  C07D239/46 ,  C07D239/42 ,  C07D239/47 ,  C07D473/40 ,  C07D487/02 ,  C07D401/12 ,  C07D239/48 ,  C07D473/16 ,  C07D473/24 ,  A61K45/06

Abstract: The present invention relates to certain pyrrolopyrimidine derivatives, pharmaceutical compositions containing them, and methods of using them, including methods for the treatment of proliferation disorders and other diseases related to the dysregulation of kinase (such as, but not limited to, EGFR (including HER), Alk, PDGFR, BLK, BMX/ETK, BTK, FLT3(D835Y), ITK, JAK1, JAK2, JAK3, TEC and TXK) and/or the respective pathways.

公开(公告)号：US20200071672A1

公开(公告)日：2020-03-05

申请号：US16490302

申请日：2018-03-03

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaoyu Zhang ,  Yama A Abassi ,  Nan Li ,  Xiaobo Wang

IPC: C12N5/077 ,  C12N13/00 ,  G01N27/02 ,  C12N5/074 ,  C12N5/00

Abstract: Methods of inducing functional maturation of immature cardiomyocytes derived from induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) by electrically pacing the immature cardiomyocytes according to a pulse profile that induces maturation until the immature cardiomyocytes mature into functionally adult cardiomyocytes.

公开(公告)号：US10449196B2

公开(公告)日：2019-10-22

申请号：US15708024

申请日：2017-09-18

Applicant: Acea Biosciences Inc.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A61K31/519 ,  C07D487/04 ,  A61K45/06

Abstract: The present invention relates to certain pyrrolopyrimidine derivatives, pharmaceutical compositions containing them, and methods of using them, including methods for the treatment of tumors and related diseases related to the dysregulation of kinase (such as EGFR (including HER), Alk, PDGFR, but not limited to) pathways.

公开(公告)号：US10261080B2

公开(公告)日：2019-04-16

申请号：US15650896

申请日：2017-07-15

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Lingbo Kong ,  Jian Wu ,  Ting Cheng ,  Yangde Qin ,  Xiaobo Wang

IPC: G01N33/569 ,  G01N15/14 ,  G01N15/10

Abstract: An optical engine its use in a bench top flow cytometer, the optical engine having a set of lasers, each focused horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same excitation plane of a flow cell, a set of optics that separate fluorescence of a same wavelength range into different locations in a focal plane of collection optics according to the different lasers by which the fluorescent light is excited; and a detector that selectively detects light from the different locations thereby distinguishing between fluorescence emitted within the same wavelength range as excited by the different lasers.

公开(公告)号：US10234392B2

公开(公告)日：2019-03-19

申请号：US15416976

申请日：2017-01-26

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Ye Chen ,  Hyunsun Chung ,  Zengqiang Li ,  Xiaobo Wang

IPC: G01N21/64 ,  G01N33/569 ,  G01N15/14 ,  G01N15/10

Abstract: An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.