公开(公告)号：US20170101660A1

公开(公告)日：2017-04-13

申请号：US15288190

申请日：2016-10-07

Applicant: GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji-woong Park ,  Sunoh Shin

IPC: C12P37/04 ,  A61K31/545 ,  A61K31/43 ,  C12N9/84 ,  C12N11/14

CPC classification number: C12P37/04 ,  C12N9/84 ,  C12N11/08 ,  C12Y305/01011

Abstract: The present disclosure relates to an enzyme-immobilized porous membrane and a preparation method of antibiotics using the same, and more specifically, to an enzyme-immobilized porous membrane prepared by immobilizing a specific enzyme through dead-end filtration, and a preparation method of antibiotics with a high yield using the enzyme-immobilized porous membrane.According to various exemplary embodiments of the present disclosure, the enzyme capable of promoting the synthesis reaction of the antibiotic substance is able to be stably immobilized in the porous membrane by passing the solution of enzyme through the membrane.In addition, it is possible to provide antibiotics with a high yield by preparing the antibiotics by passing the reactant solution through the enzyme-immobilized porous membrane.

公开(公告)号：US20100190206A1

公开(公告)日：2010-07-29

申请号：US12670792

申请日：2007-07-27

Applicant: Anupama Datla ,  Vyasarayani Williams Rajasekar ,  Pavel Kyslik ,  Stanislav Becka ,  Ashar Trupti Krishnakant ,  Zambre Sujata Yogesh ,  Kumar Nikunj

Inventor： Anupama Datla ,  Vyasarayani Williams Rajasekar ,  Pavel Kyslik ,  Stanislav Becka ,  Ashar Trupti Krishnakant ,  Zambre Sujata Yogesh ,  Kumar Nikunj

IPC: C12P37/04 ,  C12P37/00

CPC classification number: C12P35/04 ,  C12P37/04

Abstract: The present invention discloses isolation of Penicillin Acylase (PA) from Achromobacter sp CCM 4824 expressed in recombinant strain E. coli BL21 CCM 7394 bearing the recombinant plasmid pKXIP1 and processing of PA into biocatalyst useful for the industrial synthesis of antibiotics. More particularly the invention discloses a synthesis of semi-synthetic β-lactam antibiotics in the reaction mixture consisting of activated acyl-donor (D-p-hydroxyphenylglycine methyl ester or amide for Amoxicillin and Cefadroxil; D-phenylglycine methyl ester or amide for Ampicillin and Cephalexin) and nucleophile (6-APA or 7-ADCA) catalyzed by PA obtained from recombinant E. coli BL21 CCM 7394 as the biocatalyst.

Abstract translation: 本发明公开了在具有重组质粒pKXIP1的重组菌大肠杆菌BL21 CCM7394中表达的来自无色杆菌sp CCM 4824的青霉素酰基酶（PA）的分离，并将PA加工成用于工业合成抗生素的生物催化剂。 更具体地，本发明公开了在由活化的酰基供体（Dp-羟基苯基甘氨酸甲酯或阿莫西林和头孢羟氨苄的酰胺）组成的反应混合物中合成半合成的β-内酰胺抗生素; D-苯基甘氨酸甲酯或氨苯青霉素和头孢氨苄的酰胺 ）和由重组大肠杆菌BL21 CCM 7394作为生物催化剂获得的PA催化的亲核试剂（6-APA或7-ADCA）。

公开(公告)号：US07153676B1

公开(公告)日：2006-12-26

申请号：US10415197

申请日：2000-09-29

Applicant: Rodney B. Croteau ,  Kevin D. Walker ,  Anne Schoendorf ,  Mark R. Wildung

Inventor： Rodney B. Croteau ,  Kevin D. Walker ,  Anne Schoendorf ,  Mark R. Wildung

IPC: C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C12N5/00 ,  C12P37/04 ,  C12P21/00

CPC classification number: C12P17/02 ,  C12N9/1029

Abstract: Transacylase enzymes and the use of such enzymes to produce paclitaxel, related taxoids, as well as intermediates in the paclitaxel biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the transacylase enzymes.

Abstract translation: 公开了转酰胺酶和这些酶在紫杉醇生物合成途径中产生紫杉醇，相关紫杉烷以及中间体的用途。 还公开了编码转酰酶的核酸序列。

公开(公告)号：US6017726A

公开(公告)日：2000-01-25

申请号：US839327

申请日：1997-04-17

Applicant: Michael J. Conder ,  John A. Rambosek ,  Phyllis C. McAda ,  Christopher D. Reeves

Inventor： Michael J. Conder ,  John A. Rambosek ,  Phyllis C. McAda ,  Christopher D. Reeves

IPC: C12N1/19 ,  C07D501/20 ,  C12N1/15 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N15/09 ,  C12N15/52 ,  C12N15/54 ,  C12P20060101 ,  C12P35/00 ,  C12P35/06 ,  C12P37/04 ,  C12R1/82 ,  C12P35/02 ,  C12N9/84

CPC classification number: C12N9/0071 ,  C12N9/00 ,  C12N9/1029 ,  C12P35/00 ,  C12P37/04 ,  Y10S435/935

Abstract: A process for making 7-aminodeacetyl-cephalosporanic acid (7-ADAC).

Abstract translation: 制备7-氨基脱乙酰基 - 头孢菌酸（7-ADAC）的方法。

公开(公告)号：US5916762A

公开(公告)日：1999-06-29

申请号：US940984

申请日：1997-09-30

Applicant: Wilhelmus H. J. Boesten ,  Harold M. Moody ,  Eric C. Roos

Inventor： Wilhelmus H. J. Boesten ,  Harold M. Moody ,  Eric C. Roos

IPC: C07D499/00 ,  C12P37/04 ,  C07D499/18 ,  C12P37/00

CPC classification number: C07D499/00 ,  C12P37/04

Abstract: Process for the recovery of ampicillin from a mixture containing ampicillin and 6-aminopenicillic acid (6-APA), in which a mixture of ampicillin and 6-APA, with a pH higher than 7, which apart from any solid ampicillin being present is homogeneous at a pH between 7 and 8.5, is subjected to a pH lowering till a pH lower than 8.2 is reached, and the solid substance present is recovered. The process is in particular suitable to be applied to the reaction mixture which is obtained after the enzymatic acylation reaction of 6-APA with a phenylglycidine derivative as acylation agent. Pure ampicillin can thus be recovered in a simple way.

Abstract translation: 从含有氨苄青霉素和6-氨基青蒿酸（6-APA）的混合物中回收氨苄青霉素的方法，其中氨苄西林和6-APA的混合物，pH高于7，除了存在任何固体氨苄青霉素之外 在pH7〜8.5之间，进行pH降低，直至达到低于8.2的pH值，并回收存在的固体物质。 该方法特别适用于6-APA与苯基甘氨啶衍生物作酰化剂的酰化反应后获得的反应混合物。 因此，可以简单的方式回收纯氨苄青霉素。

公开(公告)号：US5874571A

公开(公告)日：1999-02-23

申请号：US903879

申请日：1997-07-31

Applicant: Wilhelmus H. J. Boesten ,  Eric C. Roos ,  Wilhelmus J. J. van den Tweel

Inventor： Wilhelmus H. J. Boesten ,  Eric C. Roos ,  Wilhelmus J. J. van den Tweel

IPC: C07D501/00 ,  C07D501/12 ,  C07D501/22 ,  C12P35/00 ,  C12P35/04 ,  C12P37/04 ,  C07D501/06

CPC classification number: C12P35/00 ,  C07D501/00

Abstract: The disclosed process is for the recovery of cephalexin from a mixture containing cephalexin and 7-aminodesacetoxy cephalosporanic acid (7-ADCA), wherein a mixture of cephalexin and 7-ADCA, with a pH higher than 7, which apart from any solid cephalexin being present is homogeneous at a pH between 7 and 8.5, is subjected to a pH modification until a pH lower than 7.8 is reached, and the solid substance is recovered. The disclosed process is particularly suited for application to a reaction mixture obtained after the enzymatic acylation reaction of 7-ADCA with a phenylglycine derivative as an acylation agent. Pure cephalexin can thus be recovered in a simple manner.

Abstract translation: 所公开的方法是从含有头孢氨苄和7-氨基二乙酰氧基头孢菌酸（7-ADCA）的混合物中回收头孢氨苄，其中除了任何固体头孢氨苄之外，pH高于7的头孢氨苄和7-ADCA的混合物 在pH7-7.5之间存在是均相的，经过pH调节，直到达到低于7.8的pH，并回收固体物质。 所公开的方法特别适用于7-ADCA与作为酰化剂的苯基甘氨酸衍生物进行酶酰化反应后得到的反应混合物。 因此可以简单的方式回收纯头孢氨苄。

公开(公告)号：US5753458A

公开(公告)日：1998-05-19

申请号：US596166

申请日：1996-02-07

Applicant: Kim Clausen ,  Annette Nielsen ,  Niels Petersen ,  Alexander Nikolov

Inventor： Kim Clausen ,  Annette Nielsen ,  Niels Petersen ,  Alexander Nikolov

IPC: C12P35/04 ,  C12P37/04 ,  C12P35/00 ,  C12P35/02 ,  C12P35/06

CPC classification number: C12P37/04 ,  C12P35/04

Abstract: A method for providing a semisynthetic .beta.-lactam antibiotic by enzyme catalyzed acylation of the parent .beta.-lactam with an activated derivative of the side chain acid wherein a modulator, which consists of one or more compounds different from the reactants and the reaction product and which suppresses the hydrolysis of the activated derivative of the side chain acid and the desired product more than it suppresses the synthesis of the desired product, is added to the reaction mixture, at the beginning of the reaction process, in a concentration from about 0.2 to 100.times.10.sup.3 .mu.m.

Abstract translation: PCT No.PCT / EP95 / 02277 Sec。 371日期：1996年1月7日 102（e）日期1996年1月7日PCT提交1995年6月12日PCT公布。 公开号WO95 / 34675 PCT 日期1995年12月21日一种提供半合成β-内酰胺抗生素的方法，其通过用活化的侧链酸衍生物酶催化母体β-内酰胺酰化，其中调节剂由一种或多种不同于反应物的化合物组成， 在反应过程开始时，将反应产物并且抑制侧链酸和所需产物的活化衍生物的水解比抑制所需产物合成更多的方法加入到反应混合物中，浓度 约0.2〜100×103亩。

公开(公告)号：US5525483A

公开(公告)日：1996-06-11

申请号：US182872

申请日：1994-01-18

Applicant: Svend G. Kaasgaard ,  Ulla Veitland

Inventor： Svend G. Kaasgaard ,  Ulla Veitland

IPC: C07D499/68 ,  C07D501/28 ,  C12P35/04 ,  C12P37/04 ,  C12R1/19 ,  C12P37/02

CPC classification number: C12P35/04 ,  C12P37/04

Abstract: .beta.-Lactam derivatives are synthesized by an enzymatic reaction of the parent amino .beta.-lactam with the corresponding acylating agent, the concentration of the acylating agent plus the concentration of .beta.-lactam derivative in the reaction mixture being above about 400 mM.

Abstract translation: β-内酰胺衍生物通过亲本氨基β-内酰胺与相应的酰化剂的酶反应合成，酰化剂的浓度加上反应混合物中β-内酰胺衍生物的浓度高于约400mM。

公开(公告)号：US3622462A

公开(公告)日：1971-11-23

申请号：US79998369

申请日：1969-02-17

Applicant: ASTRA AB

Inventor： DELIN PER STAFFAN ,  EKSTROM BERTIL AKE ,  NATHORST-WESTFELT LARS SOLVE ,  SJOBERG BERNDT OLOF HARALD ,  THELIN KARL HUGO

IPC: C07D499/00 ,  C07D499/42 ,  C12N11/10 ,  C12P37/04 ,  C07G7/02

CPC classification number: C12N11/10 ,  C07D499/00 ,  C12P37/04 ,  Y10S435/814 ,  Y10S435/815 ,  Y10S435/849

Abstract: An improved process for the large-scale production of penicillin acylase wherein the purified, cell-free enzyme is prepared by fermenting an E. coli cell strain and separating the intracellular enzyme under controlled temperature from the culture fluid by the rapid expulsion of the cell material into water through a peripheral slit from an applied pressure of at least 500 p.s.i., but not exceeding 3,000 p.s.i. The cell material is then stirred in the aqueous solution to dissolve the enzyme which is isolated as a purified acylase capable of transferring the side chain of a natural penicillin, especially benzylpenicillin, to accomplish the formation of 6aminopenicillanic acid by enzymatic degradation. Upon acylation of the 6-aminopenicillanic acid hypo-allergenic penicillins are obtained.

公开(公告)号：US3079306A

公开(公告)日：1963-02-26

申请号：US7231160

申请日：1960-11-29

Applicant: BAYER AG

Inventor： ALBERT OFFE HANS ,  WILFRIED KAUFMANN ,  KLAUS BAUER

IPC: C07D499/12 ,  C07D499/60 ,  C12P37/04

CPC classification number: C12P37/04 ,  Y10S435/849