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    Method for analyzing n-terminal protein adducts
    25.
    发明申请
    Method for analyzing n-terminal protein adducts 有权
    分析n末端蛋白加合物的方法

    公开(公告)号：US20070190659A1

    公开(公告)日：2007-08-16

    申请号：US10593449

    申请日：2005-04-13

    Applicant: Per Rydberg

    Inventor： Per Rydberg

    IPC: G01N33/00

    CPC classification number: C07D233/86 ,  G01N33/68 ,  G01N33/6809 ,  G01N33/6824 ,  G01N33/6848 ,  G01N33/72

    Abstract: Method for analyzing adducts in a fluid and/or a solid material suspected of containing the adducts by bringing the material in contact with an isothiocyanate reagent containing a fluorescent. The reagent do not have the isothiocyanate group directly bound to an unsubstituted phenyl or pentafluorophenyl group. A method for manufacturing a standard material for use in the analyzing method, a standard material obtainable by this manufacturing method. Compounds suitable for use as standard material and use of the standard material, and a container for analyzing the adducts are also disclosed. A method for analyzing hazardous substances, a kit including standard material or a compound set out above, an apparatus for performing the analyzing method, and a computer program stored on a data carrier for performing the analyzing method or the manufacturing method are also disclosed.

    Abstract translation: 通过使材料与含有荧光的异硫氰酸酯试剂接触来分析疑似含有加合物的流体和/或固体物质中的加合物的方法。 试剂不具有与未取代的苯基或五氟苯基直接结合的异硫氰酸酯基团。 一种制造用于分析方法的标准材料的方法，可通过该制造方法获得的标准材料。 还公开了适合用作标准材料和使用标准材料的化合物，以及用于分析加合物的容器。 还公开了一种用于分析有害物质的方法，包括标准材料或上述化合物的试剂盒，用于执行分析方法的装置，以及存储在用于执行分析方法或制造方法的数据载体上的计算机程序。

    Peptide analysis using a solid support
    26.
    发明申请
    Peptide analysis using a solid support 审中-公开
    使用固体支持物进行肽分析

    公开(公告)号：US20040171070A1

    公开(公告)日：2004-09-02

    申请号：US10478910

    申请日：2004-04-28

    Inventor： Ramagauri Bhikhabhai ,  Maria Liminga ,  Jean-Luc Maloisel ,  Ronnie Palmgren ,  Thomas W Keough ,  Amy M Keough ,  Robert S Youngquist ,  Harold L Vaughn ,  Ken E Yelm

    IPC: G01N033/53

    CPC classification number: G01N33/6848 ,  C07K1/12 ,  G01N33/543 ,  G01N33/6803 ,  G01N33/6818 ,  G01N33/6824 ,  G01N33/6851

    Abstract: The present invention relates to a method of identifying a polypeptide, which method comprises the steps of (a) derivatization of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern, and (c) interpreting the fragmentation pattern obtained, wherein the peptide or polypeptide is immobilized to a solid support at least during step(a). Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique.

    Abstract translation: 本发明涉及一种鉴定多肽的方法，该方法包括以下步骤：（a）使多肽的一个或多个肽的N-末端的N末端或至少一个酸性的 试剂，其包含与活化的酸部分偶联以提供一种或多种肽衍生物的磺酰基部分; 使用质谱技术分析至少一种这样的衍生物以提供分裂模式，和（c）解释获得的断裂模式，其中至少在步骤（a）期间将肽或多肽固定在固体支持物上。 此外，本发明还涉及通过质谱技术鉴定多肽的试剂盒。

    Amino acid sequence determination for protein or the like
    27.
    发明申请
    Amino acid sequence determination for protein or the like 审中-公开
    蛋白质等的氨基酸序列测定

    公开(公告)号：US20020086442A1

    公开(公告)日：2002-07-04

    申请号：US10015662

    申请日：2001-12-17

    Applicant: Shimadzu Corporation

    Inventor： Hideshi Fujiwake

    IPC: G01N033/543

    CPC classification number: G01N33/6806 ,  G01N33/6824

    Abstract: Respective monoclonal antibodies are fixed into respective wells of a microplate one by one, and bonded with labeled analogs of PTH amino acid derivatives. A solution containing PTH amino acid derivatives obtained by Edman degradation is dripped on to the microplate for causing competitive reaction to the monoclonal antibodies with the analogs, and thereafter non-bonded PTH amino acid derivatives and liberated analogs are washed out. Then enzyme-labeled antibodies against the analogs are added and the quantity of bonded enzyme-labeled antibodies is measured thereby determining the types and quantities of the PTH amino acid derivatives. Constitutive amino acid can be detected in high sensitivity.

    Abstract translation: 将各单克隆抗体逐一固定在微孔板的相应孔中，并与PTH氨基酸衍生物的标记类似物结合。 将含有通过Edman降解获得的PTH氨基酸衍生物的溶液滴加到微孔板上，引起与类似物对单克隆抗体的竞争性反应，然后将非结合的PTH氨基酸衍生物和释放的类似物洗掉。 然后加入针对类似物的酶标记抗体，并测量结合酶标记抗体的量，从而确定PTH氨基酸衍生物的类型和数量。 组氨酸可以高灵敏度检测。

    Amino acid sequencing peptides and methods for their use
    28.
    发明授权
    Amino acid sequencing peptides and methods for their use 失效
    氨基酸测序肽及其使用方法

    公开(公告)号：US5919709A

    公开(公告)日：1999-07-06

    申请号：US739819

    申请日：1996-10-30

    Applicant: David C. Parmelee ,  Salvatore Sechi

    Inventor： David C. Parmelee ,  Salvatore Sechi

    IPC: C07K1/12 ,  C07K14/00 ,  C07K14/47 ,  G01N33/68 ,  G01N33/00 ,  A61K38/00 ,  C07K7/00

    CPC classification number: C07K14/4717 ,  C07K1/128 ,  C07K14/001 ,  G01N33/6824

    Abstract: The present invention provides a novel internal standard for amino acid sequencing which contains a peptide consisting of unnatural amino acid residues, such as ornithine, norvaline, norleucine and .alpha.-aminobutyric acid, that is capable of being sequenced simultaneously with an unknown peptide or protein without interfering with the analysis. The internal standard peptide has an amino acid sequence containing at least two different unnatural amino acid residues having retention times distinct from the corresponding retention times for natural amino acid residues. Information derived from the sequencing of the internal standard allows determination of repetitive yield, lag, N-terminal blockage and discrimination between blank cycles caused by missed injection and blank cycles caused by faulty delivery of chemicals during the sequencer reactions. The present invention further provides novel synthetic control peptides containing from about 3 to 100 natural amino acid residues that are designed for use in monitoring the proper operation of amino acid sequencers and to monitor peptide or protein cleavage reactions. The control peptide, or mixture of control peptides, are designed to obtain data for many or all common, uncommon and difficult to measure amino acids within 15 sequencer cycles and to provide cleavage sites for at least 4 different amino acid cleavage reactants.

    Abstract translation: 本发明提供了一种氨基酸测序的新型内标，其包含由非天然氨基酸残基组成的肽，例如鸟氨酸，正缬氨酸，正亮氨酸和α-氨基丁酸，其能够与未知的肽或蛋白质同时测序而没有 干扰分析。 内标肽具有含有至少两个不同的天然氨基酸残基的氨基酸序列，其保留时间与天然氨基酸残基的相应保留时间不同。 从内标测序获得的信息可以确定重复产量，滞后，N末端阻塞以及在排序反应期间由错误输送化学品导致的错过注射和空白循环引起的空白循环之间的区别。 本发明还提供了新颖的合成对照肽，其含有约3至100个天然氨基酸残基，其被设计用于监测氨基酸测序仪的正常操作并监测肽或蛋白质切割反应。 设计对照肽或对照肽的混合物以在15个测序周期内获得许多或所有常见的，罕见的和难以测量氨基酸的数据，并为至少4种不同的氨基酸切割反应物提供切割位点。

    Continuous biochemical reactor for analysis of sub-picomole quantities of complex organic molecules
    29.
    发明授权
    Continuous biochemical reactor for analysis of sub-picomole quantities of complex organic molecules 失效
    用于分析亚皮摩尔量的复杂有机分子的连续生化反应器

    公开(公告)号：US5565171A

    公开(公告)日：1996-10-15

    申请号：US438433

    申请日：1995-05-10

    Applicant: Norman J. Dovichi ,  Karen C. Waldron

    Inventor： Norman J. Dovichi ,  Karen C. Waldron

    IPC: B01J19/00 ,  G01N33/68

    CPC classification number: B01J19/0093 ,  G01N33/6824 ,  Y10T436/143333 ,  Y10T436/25375 ,  Y10T436/255

    Abstract: A novel reactor for reacting and subsequently analyzing sub-picomole quantities of a sample organic molecule. The reactor includes a continuous capillary connected between two valves that control fluid flow in the capillary. One part of the capillary forms a reaction chamber where the sample may be immobilized for subsequent reaction with reagents supplied through the valves. Another part of the capillary passes through or terminates in the detector portion of an analyzer such as an electrophoresis apparatus, liquid chromatographic apparatus or mass spectrometer. The apparatus may form a peptide or protein sequencer for carrying out the Edman degradation reaction and analyzing the reaction product produced by the reaction. The protein or peptide sequencer includes a reaction chamber for carrying out coupling and cleavage on a peptide or protein to produce derivatized amino acid residue, a conversion chamber for carrying out conversion and producing a converted amino acid residue and an analyzer for identifying the converted amino acid residue. The reaction chamber may be contained within one arm of a capillary and the conversion chamber is located in another arm of the capillary. An electrophoresis length of capillary is directly capillary coupled to the conversion chamber to allow electrophoresis separation of the converted amino acid residue as it leaves the conversion chamber. Identification of the converted amino acid residue takes place at one end of the electrophoresis length of the capillary.

    Abstract translation: 一种用于反应并随后分析亚微微摩尔量的样品有机分子的新型反应器。 反应器包括连接在控制毛细管中的流体流动的两个阀之间的连续毛细管。 毛细管的一部分形成反应室，其中样品可以被固定以便随后通过阀提供的试剂反应。 毛细管的另一部分通过或终止于分析仪的检测器部分，例如电泳装置，液相色谱装置或质谱仪。 该装置可以形成用于进行埃德曼降解反应并分析由反应产生的反应产物的肽或蛋白测序仪。 蛋白质或肽测序仪包括用于在肽或蛋白质上进行偶联和切割以产生衍生的氨基酸残基的反应室，用于进行转化并产生转化的氨基酸残基的转化室和用于鉴定转化的氨基酸的分析仪 残留物 反应室可以包含在毛细管的一个臂内，并且转化室位于毛细管的另一个臂中。 毛细管的电泳长度直接毛细耦合到转化室，以便在转化的氨基酸残基离开转化室时电泳分离转化的氨基酸残基。 转化的氨基酸残基的鉴定发生在毛细管的电泳长度的一端。

    Methods for amino acid sequencing of a polypeptide
    30.
    发明授权
    Methods for amino acid sequencing of a polypeptide 失效
    氨基酸氨基酸序列测定方法

    公开(公告)号：US5240859A

    公开(公告)日：1993-08-31

    申请号：US658403

    申请日：1991-02-22

    Applicant: Rudolf H. Aebersold

    Inventor： Rudolf H. Aebersold

    IPC: C07C331/16 ,  C07C331/28 ,  C07K1/12 ,  G01N33/68

    CPC classification number: C07C331/28 ,  C07K1/128 ,  G01N33/6824 ,  Y10T436/24

    Abstract: The present invention provides methods and reagents for sequencing amino acids. One embodiment of the method for determining the terminal amino acid of a substantialy pure polypeptide comprises the steps of (a) attaching the polypeptide to a solid support, (b) reacting the polypeptide with a compound described below, under conditions and for a time sufficient for coupling to occur between the terminal amino acid of the polypeptide and the compound, thereby yielding a polypeptide with a derivatized terminal amino acid, (c) washing the solid support to remove unbound material, (d) cleaving the derivatized terminal amino acid from the polypeptide with a cleaving agent, (e) ionizing the cleaved derivatized terminal amino acid, and (f) determining the molecular weight of the derivatized terminal amino acid, such that the terminal amino acid is determined.Within one embodiment, the compound is p-isothiocyanato phenethyl trimethylammonium and counterion salts thereof.

    Abstract translation: 本发明提供了氨基酸测序方法和试剂。 用于确定实质纯多肽的末端氨基酸的方法的一个实施方案包括以下步骤：（a）将多肽连接到固体支持物上，（b）使条件和时间足够的多肽与下述化合物反应 用于偶联发生在多肽的末端氨基酸和化合物之间，从而产生具有衍生化末端氨基酸的多肽，（c）洗涤固体支持物以除去未结合的物质，（d）将衍生化的末端氨基酸从 多肽，（e）电离切割的衍生化末端氨基酸，和（f）确定衍生的末端氨基酸的分子量，从而确定末端氨基酸。 在一个实施方案中，化合物是对异硫氰酸苯乙酯三甲基铵及其抗衡离子盐。

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