公开(公告)号：US20140339421A1

公开(公告)日：2014-11-20

申请号：US14374035

申请日：2013-01-24

Applicant: Thermo Finnigan LLC

Inventor： Michael W. Senko

IPC: H01J49/00

CPC classification number: H01J49/0045 ,  G01N33/6848 ,  G06F19/18 ,  H01J49/0031 ,  H01J49/0081 ,  H01J49/428

Abstract: A mass spectrometry method for analyzing isobarically-labeled analyte compounds comprising (a) ionizing compounds including the isobarically-labeled analyte compounds to generate a plurality of precursor ion species comprising different respective m/z ratios, (b) isolating a precursor ion species, (c) fragmenting the precursor ion species to generate a plurality of first-generation fragment ion species comprising different respective m/z ratios, and (d) selecting and co-isolating two or more of the first-generation product-ion species, the method characterized by: (e) fragmenting all of the selected and isolated first-generation product ion species so as to generate a plurality of second-generation fragment ion species including released label ions; (f) generating a mass spectrum of the second-generation fragment ion species; and (g) generating quantitative information relating to at least one analyte compound based on peaks of the mass spectrum attributable to the released label ions.

Abstract translation: 一种用于分析等压标记的分析物质的质谱法，其包括（a）电离化合物，包括等量标记的分析物质，以产生包含不同相应m / z比的多种前体离子物质，（b）分离前体离子物质， c）使前体离子物质碎裂以产生包含不同相应m / z比率的多个第一代碎片离子物质，和（d）选择和共分离两种或更多种第一代产物离子种类，该方法 其特征在于：（e）将所有选择和分离的第一代产物离子物质分段，以产生多个第二代碎片离子物质，包括释放的标记离子; （f）产生第二代碎片离子物质的质谱; 和（g）基于归因于释放的标记离子的质谱的峰产生与至少一种分析物化合物相关的定量信息。

公开(公告)号：US20130299693A1

公开(公告)日：2013-11-14

申请号：US13877580

申请日：2011-10-13

Applicant: Yu Xia ,  Frank A. Londry

Inventor： Yu Xia ,  Frank A. Londry

IPC: H01J49/00

CPC classification number: H01J49/004 ,  H01J49/0031 ,  H01J49/0063 ,  H01J49/428

Abstract: A tandem mass spectrometer system and method are described, where a composite voltage waveform is applied to so as to trap ion having selected m/z. The trapped ions may be subject to collision induced ionization dissociation (CID) by a selectable discrete frequency voltage waveform positioned so as to be in a notch in a broadband waveform. The resultant ion products may be trapped using a second notch having a center frequency corresponding to the ion product to be trapped. The process may be repeated so as to increase the amount of ions produced, or the process a first resultant ion product to yield a second resultant in product, which may be trapped.

Abstract translation: 描述了串联质谱仪系统和方法，其中施加复合电压波形以捕获具有选定m / z的离子。 被捕获的离子可能通过可选的离散频率电压波形进行碰撞感应电离解离（CID），该波形定位成宽带波形中的陷波。 可以使用具有对应于待捕获的离子产物的中心频率的第二缺口来捕获所得的离子产物。 可以重复该过程，以便增加产生的离子的量，或者加工第一合成的离子产物以产生可能被捕获的第二产物。

公开(公告)号：US08288720B2

公开(公告)日：2012-10-16

申请号：US13219408

申请日：2011-08-26

Applicant: Junichi Taniguchi

Inventor： Junichi Taniguchi

IPC: B01D59/44 ,  H01J49/42 ,  H01J49/40

CPC classification number: H01J49/424 ,  H01J49/428

Abstract: A technique for performing precursor isolation with a desired mass-to-charge ratio (m/z) in a digital ion trap while maintaining the q value at a substantially constant value is provided. A data obtained by digitizing an FNF signal having a notch is stored beforehand in an FNF waveform memory 15. In the process of precursor isolation, a main voltage timing controller 7 and a main voltage generator 9 generate a rectangular-wave voltage based on a reference clock signal CK. An auxiliary signal generator 14 reads data from the FNF waveform memory 15 and generates an FNF signal by performing digital-to-analogue conversion of the data in accordance with a clock signal synchronized with the reference clock signal CK. Under the command of a controller 30, a reference clock generator 6 produces the reference clock signal CK having a frequency corresponding to the m/z value of a target ion. Accordingly, a change in the m/z of the target ion leads to a change in the frequency of the reference clock signal CK, which causes the frequency of the rectangular-wave voltage and the central frequency of the notch of the FNF signal to change in the same proportion.

Abstract translation: 提供了一种用于在数字离子阱中以期望的质荷比（m / z）进行前体隔离的技术，同时将q值保持在基本恒定的值。 通过数字化具有凹口的FNF信号获得的数据预先存储在FNF波形存储器15中。在前驱体隔离的过程中，主电压定时控制器7和主电压发生器9基于参考产生矩形波电压 时钟信号CK。 辅助信号发生器14从FNF波形存储器15读取数据，并且通过根据与参考时钟信号CK同步的时钟信号对数据进行数模转换来产生FNF信号。 在控制器30的指令下，参考时钟发生器6产生具有对应于目标离子的m / z值的频率的参考时钟信号CK。 因此，目标离子的m / z的变化导致参考时钟信号CK的频率的变化，这导致矩形波电压的频率和FNF信号的陷波的中心频率改变 以相同的比例。

公开(公告)号：US20100084548A1

公开(公告)日：2010-04-08

申请号：US11920062

申请日：2006-05-01

Applicant: Scott A. McLuckey ,  Paul A. Chrisman ,  Sharon J. Pitteri

Inventor： Scott A. McLuckey ,  Paul A. Chrisman ,  Sharon J. Pitteri

IPC: H01J49/42 ,  H01J49/00

CPC classification number: H01J49/428 ,  H01J49/0072

Abstract: A method of controlling ion parking in an ion trap includes generating a trapping field for trapping cations and anions, and applying a tailored waveform during a period when ion/ion reactions occur to park first generation product ions with m/z values that differ from those of a cation and an anion in selected m/z regions. In particular, the tailored waveform inhibits simultaneously the reactions of ions of disparate m/z ratios.

Abstract translation: 在离子阱中控制离子停放的方法包括产生用于捕获阳离子和阴离子的捕获场，并且在发生离子/离子反应的时间段期间施加定制的波形以将第一代产物离子与m / z值不同的m / z值 的选择的m / z区域中的阳离子和阴离子。 特别地，定制的波形同时抑制不同m / z比的离子的反应。

公开(公告)号：US07064323B2

公开(公告)日：2006-06-20

申请号：US10495044

申请日：2003-06-27

Applicant: Hideo Yamakoshi ,  Hiroshi Futami ,  Minoru Danno ,  Shigenori Tsuruga ,  Shizuma Kuribayashi

Inventor： Hideo Yamakoshi ,  Hiroshi Futami ,  Minoru Danno ,  Shigenori Tsuruga ,  Shizuma Kuribayashi

IPC: H01J49/00 ,  B01D59/44 ,  C12M1/34

CPC classification number: H01J49/162 ,  F23J2215/301 ,  H01J49/0063 ,  H01J49/424 ,  H01J49/428

Abstract: A vacuum ultraviolet lamp ionizes a chemical substance contained in exhaust gas Gs. The chemical substance ionized is trapped in an ion trapping apparatus in which a radio frequency electric field is formed. Energy is applied to an ion group in the ion trapping apparatus with a SWIFT waveform comprising a frequency component excluding a frequency corresponding to an orbital resonance frequency of ions of the chemical substance to remove an impurity. Energy is then applied to the ion group with a TICKLE waveform having a frequency component corresponding to the orbital resonance frequency of the ions of the chemical substance to fragmentate the ions of the chemical substance. A mass of the fragment is then measured with a mass spectrometer to identify the chemical substance.

Abstract translation: 真空紫外灯照射废气Gs中含有的化学物质。 电离的化学物质被捕获在形成射频电场的离子俘获装置中。 能量被施加到离子俘获装置中的离子组，其SWIFT波形包括除了与化学物质的离子的轨道共振频率相对应的频率的频率分量以除去杂质。 然后用具有对应于化学物质的离子的轨道共振频率的频率分量的TICKLE波形将能量施加到离子组，以使化学物质的离子破碎。 然后用质谱仪测量该片段的质量以鉴定化学物质。

公开(公告)号：US06627875B2

公开(公告)日：2003-09-30

申请号：US09840633

申请日：2001-04-23

Applicant: Noubar B. Afeyan ,  Scott McLuckey ,  Fred E. Regnier

Inventor： Noubar B. Afeyan ,  Scott McLuckey ,  Fred E. Regnier

IPC: B01D5944

CPC classification number: H01J49/0077 ,  G01N30/72 ,  H01J49/4265 ,  H01J49/428

Abstract: The methods of the invention exploit the mass-to-charge ratio dependent motion of ions in an electrodynamic ion traps in synergy with a charge quenching process. In an electrodynamic ion trap, each mass-to-charge ratio is characterized by a unique set of frequencies of motion. Accordingly, by tailoring the time-varying electrical potential of the ion trap the invention controls which ions are allowed or retained in the ion trap and which ions are subjected to charge quenching reactions. Control of ion retention and charge quenching is used to improve sample throughput, dynamic mass range and signal discrimination in the mass spectrometry of multiply charged ions.

公开(公告)号：US20020195559A1

公开(公告)日：2002-12-26

申请号：US10170687

申请日：2002-06-14

Applicant: Shimadzu Corporation

Inventor： Kozo Miseki

IPC: H01J049/42

CPC classification number: H01J49/424 ,  H01J49/428

Abstract: In an ion trap mass spectrometer including an ion trap space surrounded by a ring electrode and two end cap electrodes placed opposite each other with the ring electrode between them, a method of trapping object ions of a predetermined mass-to-charge ratio in the ion trap space more assuredly and effectively. The method includes the steps of: applying an RF voltage to the ring electrode to trap the object ions; and applying an auxiliary AC voltage to the end cap electrodes, where the auxiliary voltage has a frequency spectrum with a first notch at the basic frequency of the object ions and a second notch at a a beat frequency. Then the second stage is performed where another auxiliary AC voltage of the beat frequency is applied to the end cap electrodes to expel non-object ions still remaining in the ion trap space.

Abstract translation: 在离子阱质谱仪中，包括由环形电极和两个端盖电极围绕的离子阱空间，两个端盖电极与它们之间的环形电极相对放置，在离子中捕获预定质荷比的物体离子的方法 陷阱空间更加放心和有效。 该方法包括以下步骤：向环形电极施加RF电压以捕获物体离子; 以及向所述端盖电极施加辅助AC电压，其中所述辅助电压具有在所述物体离子的基本频率处具有第一陷波的频谱和以拍频的第二陷波。 然后执行第二阶段，其中将拍频的另一辅助AC电压施加到端帽电极以排出仍然留在离子阱空间中的非对象离子。

公开(公告)号：US06194716B1

公开(公告)日：2001-02-27

申请号：US09143399

申请日：1998-08-28

Applicant: Yasuaki Takada ,  Takayuki Nabeshima ,  Minoru Sakairi ,  Yuichiro Hashimoto

Inventor： Yasuaki Takada ,  Takayuki Nabeshima ,  Minoru Sakairi ,  Yuichiro Hashimoto

IPC: B01D5944

CPC classification number: H01J49/424 ,  H01J49/0009 ,  H01J49/428

Abstract: A method for performing a mass calibration under an application of a desired ion peak position of mass spectrum attained by a mass scanning of ions of substance having a known value of m/z and the known value of m/z is added with a step for ejecting an amount of unnecessary ions not contributing to the aforesaid mass calibration accumulated in the aforesaid ion trap type mass analysis region prior to the aforesaid mass scanning operation. An accumulation of a large amount of unnecessary ions in the ion trap type mass analysis region is restricted, a disturbance of electric field in the ion trap mass analysis region generated under an influence of a spatial charge caused by accumulation of the unnecessary ions can be prevented and the aforesaid mass calibration can be performed in an easy and accurate manner.

Abstract translation: 在通过具有已知m / z值的物质的离子的质量扫描和已知的m /​​ z值获得的质谱所需离子峰位置的施加下进行质量校准的方法添加了用于 在上述质量扫描操作之前，排出在上述离子阱型质量分析区域中累积的对上述质量校准无贡献的不需要的离子的量。 离子阱型质量分析区域中的大量不需要的离子的积聚受到限制，可以防止在由不需要的离子的积聚引起的空间电荷的影响下产生的离子阱质量分析区域中的电场的扰动 并且可以容易且准确地进行上述质量校准。

公开(公告)号：US5654542A

公开(公告)日：1997-08-05

申请号：US588209

申请日：1996-01-19

Applicant: Michael Schubert ,  Jochen Franzen

Inventor： Michael Schubert ,  Jochen Franzen

IPC: H01J49/42

CPC classification number: H01J49/428

Abstract: A method for the simultaneous resonant excitation of the oscillations of ions of various mass-to-charge ratios in ion traps, particularly for the ejection of undesirable ion species, by applying RF frequencies with various frequency components to electrodes of the ion trap. The method consists in generating and storing a broadband signal for as short a time as possible so that it can be fed to the ion trap a number of times in succession cyclically, without generating undesirable interference due to phase shifts. The excitation of the ions should be as temporally constant as possible throughout the waveform period. The duration of the waveform period depends on the mass resolution required. Excitation is terminated by controlling the broadband signal gradually toward zero in a constant function.

Abstract translation: 通过将具有各种频率分量的RF频率施加到离子阱的电极，同时共振激发离子阱中各种质荷比的离子的振荡，特别是用于喷射不期望的离子种类的方法。 该方法包括在尽可能短的时间内产生和存储宽带信号，使得其可以循环地连续多次馈送到离子阱，而不会由于相移而产生不期望的干扰。 在整个波形周期内，离子的激发应该尽可能地保持恒定。 波形周期的持续时间取决于所需的质量分辨率。 通过在恒定功能中将宽带信号逐渐向零控制来终止激励。

公开(公告)号：US5521379A

公开(公告)日：1996-05-28

申请号：US277666

申请日：1994-07-20

Applicant: Jochen Franzen ,  Reemt-Holger Gabling

Inventor： Jochen Franzen ,  Reemt-Holger Gabling

IPC: B01J19/08 ,  H01J49/42

CPC classification number: H01J49/428 ,  B01J19/087 ,  H01J49/145 ,  H01J49/4295

Abstract: A method is disclosed for chemical ionization of the molecules of an analysis gas or analysis gas mixture or for studying ion-molecule reactions in ion traps. Specifically, by superposing a quadrupole field with a dipolar introduced frequency mixture in which there are accurately selected frequency gaps in the ionization phase of the analysis, the only ions which are stored are those which are on the selected reaction path of the reactant gas ions for making the proper reactant ions for the desired ion-molecule reaction. All the other ions are eliminated from storage by excitation of their secular oscillations. In a second phase, by switching off the dipolar introduced frequency mixture, all the ions can be stored in a wide range of mass-to-charge ratios, and therefore also the product ions of the desired ion-molecule reactions of the analysis gas. In a third phase the spectrum of the product ions is scanned.

Abstract translation: 公开了用于分析气体或分析气体混合物的分子的化学电离或用于研究离子阱中的离子 - 分子反应的方法。 具体地说，通过将具有偶极引入频率混合的四极场叠加在分析的电离阶段中存在准确选择的频率间隙的情况下，存储的唯一离子是在反应物气体离子的选定反应路径上 使合适的反应物离子进行所需的离子 - 分子反应。 所有其他离子通过其长期振荡的激发从储存中消除。 在第二阶段，通过关闭偶极引入的频率混合物，所有离子可以以宽范围的质荷比存储，因此也可以分析气体所需的离子 - 分子反应的产物离子。 在第三阶段，扫描产物离子的光谱。