公开(公告)号：US06277644B1

公开(公告)日：2001-08-21

申请号：US09273945

申请日：1999-03-22

Applicant: Vincent R. Farnsworth ,  Paul K. Cartier, III

Inventor： Vincent R. Farnsworth ,  Paul K. Cartier, III

IPC: G01N3368

CPC classification number: G01N33/6818 ,  G01N33/6824 ,  Y10S436/808

Abstract: A modified Edman degradation process is used to obtain compositional tags for proteins. The Edman degradation chemistry is separated from amino acid analysis, circumventing the serial requirement of the conventional Edman process. Multiple cycles of coupling and cleavage are performed prior to extraction and compositional analysis of amino acids. The amino acid composition information is used to search a database of known protein or DNA sequences to identify the sample protein.

Abstract translation: 改进的Edman降解过程用于获得蛋白质的组成标签。 埃德曼降解化学与氨基酸分析分离，避免了传统埃德曼法的连续要求。 在提取和氨基酸组成分析之前进行多次偶联和切割循环。 使用氨基酸组成信息来搜索已知蛋白质或DNA序列的数据库以鉴定样品蛋白质。

公开(公告)号：US5922608A

公开(公告)日：1999-07-13

申请号：US690667

申请日：1996-07-31

Applicant: Vincent R. Farnsworth ,  Wulf L. Carson

Inventor： Vincent R. Farnsworth ,  Wulf L. Carson

IPC: G01N33/68 ,  G01N33/545

CPC classification number: G01N33/6818 ,  Y10T428/21 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T428/2998 ,  Y10T436/255

Abstract: A method, apparatus and article for sequencing polypeptides and proteins ("macromolecules") utilizes a sequencing packet comprised of a plurality of substrates having two different derivatization chemistries within a forarninous container. The sequencing packet is disposed within a reaction chamber in a cartridge assembly having an inlet and outlet. An unknown target macromolecule, and preferably a control macromolecule are bonded to the substrates. The macromolecule and control are then simultaneously sequenced. The control serves to monitor the efficacy of the sequencing process.

Abstract translation: 用于测序多肽和蛋白质的方法，装置和制品（“大分子”）利用由在多孔容器内具有两种不同的衍生化学物质的多个底物组成的测序包。 排序包被设置在具有入口和出口的盒组件中的反应室内。 未知的目标大分子，优选对照高分子结合到基材上。 然后将大分子和对照同时测序。 该控制用于监测测序过程的功效。

公开(公告)号：US4911195A

公开(公告)日：1990-03-27

申请号：US205772

申请日：1988-06-13

Applicant: Vincent R. Farnsworth ,  Paul K. Cartier, III

Inventor： Vincent R. Farnsworth ,  Paul K. Cartier, III

IPC: B01J4/00 ,  B01J19/00 ,  C40B40/10 ,  C40B60/14

CPC classification number: B01J4/001 ,  B01J19/0046 ,  B01J2219/00394 ,  B01J2219/00398 ,  B01J2219/0059 ,  B01J2219/00725 ,  C40B40/10 ,  C40B60/14 ,  Y10T137/3124 ,  Y10T137/314 ,  Y10T137/87684 ,  Y10T137/87917

Abstract: A valve block assembly with internal delivery channels and a common outlet channel is provided. Access of reactants to the delivery channels is controlled by diaphragm valves, preferably equipped with relief grooves to accommodate deformation of the diaphragm. Reservoirs feeding the delivery channels have an inert atmosphere controlled by pressurizing and venting channels in the valve block. Exposure of plastic tubing to oxygen is kept to a minimum. The assembly is particularly adapted for use in a DNA or protein sequencer to supply reactants without cross-contamination or oxidative decomposition.

Abstract translation: 提供具有内部输送通道和公共出口通道的阀块组件。 反应物到达输送通道的通道由隔膜阀控制，隔膜阀优选配备有释放槽以适应隔膜的变形。 供给输送通道的储存器具有通过对阀块中的通道加压和排放来控制的惰性气氛。 将塑料管暴露在氧气中保持最小。 该组合物特别适用于DNA或蛋白质测序仪，以提供反应物而不会发生交叉污染或氧化分解。