公开(公告)号：US10724988B2

公开(公告)日：2020-07-28

申请号：US13900712

申请日：2013-05-23

Applicant: Tecan Trading AG

Inventor： Marc N. Feiglin

IPC: G01N27/447 ,  B01L3/00

Abstract: Digital microfluidics system manipulates samples in liquid droplets within disposable cartridges that have bottom layer, top layer, and gap between the bottom and top layers. The system has a base unit with cartridge accommodation sites and a central control unit for controlling selection of individual electrodes of electrode arrays and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the cartridges by electrowetting. The system further has board accommodation sites located at the cartridge accommodation sites that each can take up a swappable electrode board having an electrode array and electrical board contact elements individually connected to electrodes of the electrode array. Each board accommodation site has electrical base unit contact elements that are connected to the central control unit and that are configured to engage with the electrical board contact elements of a swappable electrode board that is placed at the board accommodation site.

公开(公告)号：US09783842B2

公开(公告)日：2017-10-10

申请号：US14372796

申请日：2013-01-18

Applicant: TECAN TRADING AG

Inventor： Joseph Mamone ,  Marc N. Feiglin ,  Howard Gamper

IPC: C12Q1/68

CPC classification number: C12Q1/6813 ,  C12Q1/6827 ,  C12Q2525/151 ,  C12Q2525/186

Abstract: In a method of deducing the number of repeat units in a selected short tandem repeat (STR) in a genomic sample, at least a single stranded target DNA generated from a genomic sample comprising a selected STR, an STR probe (P1,P1′), a reference probe (P2), and two blockers (B1,B2) are provided, and at least three differential hybridization experiments are carried out, based on which the number of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in each differential hybridization experiment is determined. The method further comprises the step of comparing these numbers of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in the differential hybridization experiments for deducing the number of repeat units in the selected STR on the single stranded target DNA strand. Also disclosed are kits for carrying out STR genotyping by differential hybridization.

公开(公告)号：US09435765B2

公开(公告)日：2016-09-06

申请号：US13737183

申请日：2013-01-09

Applicant: TECAN TRADING AG

Inventor： S. Nicholas Reimitz ,  Bruce Richardson ,  Marc N. Feiglin ,  Anne R. Kopf-Sill

IPC: G01N27/447 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N27/44782 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502792 ,  B01L3/5029 ,  B01L3/523 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/141 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0683

Abstract: A cartridge manipulates samples in liquid droplets with an electrode array when a working film is placed on the array. The cartridge has a body with lower surface and wells to hold samples, each with a bottom opening to release liquid. A piercable bottom structure seals the bottom openings. A working film below the body has a hydrophobic upper surface. A peripheral spacer connects the working film to the body and forms a gap is between the body and surface. A top piercing system located in at least one of the wells has a piston and a piercing element, the piston being movable in the well and the piercing element piercing the piercable bottom structure for releasing a sample from a well into the gap.

Abstract translation: 当工作薄膜放置在阵列上时，墨盒用电极阵列操纵液滴样品。 墨盒具有下表面的主体和孔，用于容纳样品，每个具有底部开口以释放液体。 可穿孔底部结构密封底部开口。 身体下方的工作膜具有疏水性上表面。 外围间隔件将工作薄膜连接到主体，并在主体和表面之间形成间隙。 位于至少一个井中的顶部穿孔系统具有活塞和穿孔元件，活塞可在井中移动，穿孔元件刺穿可穿透的底部结构，以将样品从井中释放到间隙中。

公开(公告)号：US20150010908A1

公开(公告)日：2015-01-08

申请号：US14372796

申请日：2013-01-18

Applicant: TECAN TRADING AG

Inventor： Joseph Mamone ,  Marc N. Feiglin ,  Howard Gamper

IPC: C12Q1/68

CPC classification number: C12Q1/6813 ,  C12Q1/6827 ,  C12Q2525/151 ,  C12Q2525/186

Abstract: In a method of deducing the number of repeat units in a selected short tandem repeat (STR) in a genomic sample, at least a single stranded target DNA generated from a genomic sample comprising a selected STR, an STR probe (P1,P1′), a reference probe (P2), and two blockers (B1,B2) are provided, and at least three differential hybridization experiments are carried out, based on which the number of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in each differential hybridization experiment is determined. The method further comprises the step of comparing these numbers of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in the differential hybridization experiments for deducing the number of repeat units in the selected STR on the single stranded target DNA strand. Also disclosed are kits for carrying out STR genotyping by differential hybridization.

Abstract translation: 在基因组样品中推定所选短串联重复序列（STR）中的重复单元数的方法中，至少由包含选定的STR，STR探针（P1，P1'）的基因组样品产生的单链靶DNA， ，提供参考探针（P2）和两个阻断剂（B1，B2），并进行至少三个差异杂交实验，基于此，每个靶DNA链结合的STR探针寡核苷酸（P1，P1'）的数量 在每个差异杂交实验中确定。 该方法还包括在差异杂交实验中比较在每个靶DNA链上结合的STR探针寡核苷酸（P1，P1'）数目，以推断单链靶DNA DNA链上所选STR中重复单元数目的步骤。 还公开了用于通过差异杂交进行STR基因分型的试剂盒。