公开(公告)号：US20200096756A1

公开(公告)日：2020-03-26

申请号：US16472229

申请日：2017-12-22

Applicant: Leica Microsystems CMS GmbH

Inventor： Frank SIECKMANN

IPC: G02B21/26 ,  G02B21/34 ,  G02B21/24

Abstract: A holder for a microscope slide includes a receiving area that has a first contact surface and a second contact surface opposite the first contact surface, a first counter-surface that at least partially spans the first contact surface, and a second counter-surface that at least partially spans the second contact surface. The receiving area is bounded on three sides by side elements and has an opening on one side for insertion of the microscope slide. At least one pressure element is arranged within the receiving area and configured to exert a restoring force directed towards an interior of the receiving area.

公开(公告)号：US20240411123A1

公开(公告)日：2024-12-12

申请号：US18705602

申请日：2021-11-02

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Frank SIECKMANN ,  Frank HECHT

IPC: G02B21/36

Abstract: A method for providing position information for retrieving a target position in a microscopic sample includes providing a first representation of the sample at a first resolution including the target position; specifying a first target position identifier indicating the target position at the first resolution; acquiring an image stack comprising the target position indicated by the first target position identifier; providing a second representation at a second resolution higher than the first resolution based on the image stack; specifying a second target position identifier indicating the target position at the second resolution; specifying a plurality of reference position identifiers in the second representation indicating positions of optically detectable reference markers at the second resolution; and determining a set of geometric descriptors describing spatial relations between the second target position identifier and the plurality of reference position identifiers to provide the position information.

公开(公告)号：US20220214533A1

公开(公告)日：2022-07-07

申请号：US17567174

申请日：2022-01-03

Applicant: Leica Microsystems CMS GmbH

Inventor： Frank SIECKMANN ,  Frederik NITSCHKE

IPC: G02B21/36 ,  G06N3/08 ,  G06T7/11 ,  G06T7/20 ,  G06T7/33

Abstract: A method for approximating image data is disclosed. The method includes obtaining initial image data formed by imaging at least part of a sample and analysing the initial image data to form one or more portions of image data. The method also includes accessing a set of predefined stereotype elements , determining one or more of the stereotype elements as a visual approximation for the at least one object within the sample, selecting one or more of the determined stereotype elements to be associated with at least one object within the sample, and forming instruction data based on and/or including the one or more selected stereotype elements, the instruction data including instructions on how to arrange the one or more selected stereotype elements, to a processing system for approximating image data and to a system comprising such processing system.

公开(公告)号：US20220028116A1

公开(公告)日：2022-01-27

申请号：US17294692

申请日：2019-11-20

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Frank SIECKMANN ,  Constantin KAPPEL

IPC: G06T7/80 ,  G02B21/36 ,  G02B21/24 ,  G06N3/04

Abstract: A method for determining a focus position includes recording at least one first image, wherein image data of the at least one recorded first image are dependent on at least one first focus position during the recording of the at least one first image. A second focus position is determined based on an analysis of the at least one recorded first image using a trained model. At least one second image is recorded using the second focus position. The at least one first image and the at least one second image contain items of information which are in a context with a training of the trained model.

公开(公告)号：US20210342636A1

公开(公告)日：2021-11-04

申请号：US17285477

申请日：2019-09-25

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Frank SIECKMANN ,  Constantin KAPPEL

IPC: G06K9/62 ,  G06N20/00 ,  G02B21/36

Abstract: A method for optimizing a workflow of at least one microscope or microscope system includes a step a) of implementing a workflow by one or more components of at least one microscope and/or microscope system, wherein the workflow comprises a capture of first data. In a step b), a trained model is determined for the workflow, at least in part based on the captured first data.

公开(公告)号：US20160048012A1

公开(公告)日：2016-02-18

申请号：US14778926

申请日：2014-03-20

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Werner KNEBEL ,  Wernher FOUQUET ,  Frank SIECKMANN

IPC: G02B21/32 ,  G02B26/08 ,  G02B21/00

CPC classification number: G02B21/32 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/367 ,  G02B26/0816

Abstract: The invention relates to a method in which a sample is manipulated with manipulation light, and in which the sample is imaged by means of the SPIM technique under illumination with illumination light, in particular excitation light for fluorescence excitation, in the form of an illumination light sheet. The method is notable for the fact that both the manipulation light and the illumination light are focused by the same objective that is arranged in an objective working position, or by different objectives that are brought successively into an objective working position; and that the manipulation light and/or the illumination light, after passing through the objective, is diverted by means of a diverting device in such a way that it propagates at an angle different from zero degrees with respect to the optical axis of the objective.

Abstract translation: 本发明涉及一种方法，其中用操作光对样品进行操作，并且其中采用照明光照射下的SPIM技术成像样品，特别是用于荧光激发的激发光，以照明光的形式 片。 该方法的特征在于，操作光和照明光均被设置在目标工作位置的相同目标或通过连续进入目标工作位置的不同目标聚焦; 并且操纵光和/或照明光在通过物镜之后通过转向装置转向，使得其以相对于物镜的光轴不成零的角度传播。