公开(公告)号：US08128892B2

公开(公告)日：2012-03-06

申请号：US13219823

申请日：2011-08-29

Applicant: David E. Butz ,  Gregory Mathus ,  Richard Cote ,  George P. Kalmakis

Inventor： David E. Butz ,  Gregory Mathus ,  Richard Cote ,  George P. Kalmakis

IPC: B01L3/00 ,  B01L3/02 ,  G01N1/14

CPC classification number: B01L3/0237 ,  B01L3/0217 ,  B01L3/0227 ,  B01L2200/022

Abstract: A hand-held, multi-channel pipettor has an electronically controlled motor to reposition pipette tips for different center to center spacing. Each repositionable tip fitting assembly has a cam following pin that is driven by cam tracks in a motor driven roller drum. Stationary ports for the multiple aspiration cylinders are strategically placed to simplify management of flexible tubes leading to the repositionable pipette tip fitting assemblies. The pipettor has a user interface that can be operated conveniently by one hand to reposition pipette tips. It has a pipette tip ejection mechanism with a sinusoidal stripper bar.

Abstract translation: 手持式多通道移液器具有电子控制电机，用于重新定位不同中心间距的吸头。 每个可重新定位的尖端配件组件具有凸轮跟随销，该凸轮跟随销由马达驱动滚筒中的凸轮轨道驱动。 用于多个抽吸气瓶的固定端口有策略地放置，以简化导向可重新定位的移液管尖端配件组件的柔性管的管理。 移液器具有可以用一只手方便地操作以重新定位移液器吸头的用户界面。 它具有带有正弦剥离器条的移液管尖端喷射机构。

公开(公告)号：US20100034706A1

公开(公告)日：2010-02-11

申请号：US12578714

申请日：2009-10-14

Applicant: Gregory Mathus ,  Terrence Kelly ,  Richard Cote

Inventor： Gregory Mathus ,  Terrence Kelly ,  Richard Cote

IPC: B01L3/02

CPC classification number: B01L3/0217 ,  B01L3/0275 ,  B01L3/0279 ,  B01L2200/023 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2200/087 ,  B01L2300/027 ,  Y10T137/0318 ,  Y10T436/2575

Abstract: In one aspect, the invention relates to a pipette tip mounting shaft configuration and a disposable pipette tip having a matching configuration. The mounting shaft includes a locking section located above a lower sealing section. The locking section has outwardly extending locking lobes located above a stop member and a lower sealing section located below the stop member. In certain embodiments, the diameter of the mounting shaft below the stop member is reduced in order to lessen insertion and ejection forces, which is particularly helpful for hand-held multi-channel pipettors. In these embodiments, the lower sealing section contains either a frustoconical sealing section, or an annular groove and a sealing ring, such as a flouroelastomeric O-ring seal. When the mounting shaft is fully inserted into the collar of the mating disposable pipette tip, the tip locks onto the mounting shaft. The bore of the pipette tip includes a circumferential shelf or shoulder separating its upper collar from the tip sealing area which is located below the circumferential shelf. The tip collar preferably includes a locking ring located at or near the upper opening for the mounting shaft. The dimensions of the collar, and in particular the distance between its circumferential shelf and the locking ring, are selected to match the dimensions of the mounting shaft between the stop member and the upper end of the locking lobes, thus locking the pipette tip in a secure, reliable position and orientation while using ergonomic insertion and ejection forces. The locking lobes preferably include a ramp portion that gently flexes and distorts the pipette tip collar out of round as the mounting shaft is inserted in the pipette tip, rather than stretching the tip collar. The circumferential shelf on the pipette tip between the tip collar and the barrel isolates the sealing area at the upper end of the barrel from distortion, thus facilitating reliable sealing engagement between the lower sealing section of the mounting shaft and the sealing ring in the upper end of the barrel of the pipette tip.

公开(公告)号：US20090071266A1

公开(公告)日：2009-03-19

申请号：US11856231

申请日：2007-09-17

Applicant: Gary E. Nelson ,  George P. Kalmakis ,  R. Laurence Keene ,  Joel Novak ,  Kenneth Steiner ,  Jonathon Finger ,  Gregory Mathus ,  Richard Cote

Inventor： Gary E. Nelson ,  George P. Kalmakis ,  R. Laurence Keene ,  Joel Novak ,  Kenneth Steiner ,  Jonathon Finger ,  Gregory Mathus ,  Richard Cote

IPC: G01N1/14 ,  B01L3/02

CPC classification number: B01L3/0234 ,  B01L2200/0605 ,  B01L2200/087 ,  B01L2200/148

Abstract: A hand-held electronic pipettor is designed particularly to be programmed and operated with one hand for the convenience of the user. It uses a capacitance touchpad control for programming, and a separate run button for the operating mode. Internal components are located so that the center of gravity of the pipettor is located within the palm of the user. Flash memory stores an empirically derived table that correlates aspiration volume to motor steps and a separate empirically derived table the correlates dispensing volumes to motor steps.

Abstract translation: 手持式电子移液器特别设计为一方面进行编程和操作，方便用户使用。 它使用电容式触摸板控件进行编程，另外还有一个运行模式的运行按钮。 内部部件被定位成使得移液器的重心位于使用者的手掌内。 闪存存储经验导出的表，其将抽吸体积与运动步骤相关联，以及单独的经验派生表将分配体积与运动步骤相关联。

公开(公告)号：US20080286157A1

公开(公告)日：2008-11-20

申请号：US11934381

申请日：2007-11-02

Applicant: Gregory Mathus ,  Terrence Kelly ,  Richard Cote

Inventor： Gregory Mathus ,  Terrence Kelly ,  Richard Cote

IPC: B01L3/02

CPC classification number: B01L3/0217 ,  B01L3/0275 ,  B01L3/0279 ,  B01L2200/023 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2200/087 ,  B01L2300/027 ,  Y10T137/0318 ,  Y10T436/2575

Abstract: In one aspect, the invention relates to a pipette tip mounting shaft configuration and a disposable pipette tip having a matching configuration. The mounting shaft includes a locking section located above a lower sealing section. The locking section has outwardly extending locking lobes located above a stop member and a lower sealing section located below the stop member. In certain embodiments, the diameter of the mounting shaft below the stop member is reduced in order to lessen insertion and ejection forces, which is particularly helpful for hand-held multi-channel pipettors. In these embodiments, the lower sealing section contains either a frustoconical sealing section, or an annular groove and a sealing ring, such as a flouroelastomeric O-ring seal. When the mounting shaft is fully inserted into the collar of the mating disposable pipette tip, the tip locks onto the mounting shaft. The bore of the pipette tip includes a circumferential shelf or shoulder separating its upper collar from the tip sealing area which is located below the circumferential shelf. The tip collar preferably includes a locking ring located at or near the upper opening for the mounting shaft. The dimensions of the collar, and in particular the distance between its circumferential shelf and the locking ring, are selected to match the dimensions of the mounting shaft between the stop member and the upper end of the locking lobes, thus locking the pipette tip in a secure, reliable position and orientation while using ergonomic insertion and ejection forces. The locking lobes preferably include a ramp portion that gently flexes and distorts the pipette tip collar out of round as the mounting shaft is inserted in the pipette tip, rather than stretching the tip collar. The circumferential shelf on the pipette tip between the tip collar and the barrel isolates the sealing area at the upper end of the barrel from distortion, thus facilitating reliable sealing engagement between the lower sealing section of the mounting shaft and the sealing ring in the upper end of the barrel of the pipette tip.

公开(公告)号：US06391241B1

公开(公告)日：2002-05-21

申请号：US08870313

申请日：1997-06-06

Applicant: Richard Alexander Cote ,  Gregory Mathus ,  Alfred L. Michaelsen

Inventor： Richard Alexander Cote ,  Gregory Mathus ,  Alfred L. Michaelsen

IPC: B29C4514

CPC classification number: B01L3/5025 ,  B01L3/50255 ,  Y10S264/48

Abstract: The invention provides for a method for the manufacture of multiwell plates and multiwell filter plates. The plates are the result of a two part construction in which individual filter discs are securely pinned between opposing plates, one of which is insert molded against the other.

Abstract translation: 本发明提供了制造多孔板和多孔过滤板的方法。 这些板是两部分结构的结果，其中单个过滤盘被牢固地固定在相对的板之间，其中一个是相对于另一个插入成型的。

公开(公告)号：US06372293B1

公开(公告)日：2002-04-16

申请号：US09399405

申请日：1999-09-20

Applicant: Gregory Mathus ,  Daniel J. Seguin ,  Victor A. Torti

Inventor： Gregory Mathus ,  Daniel J. Seguin ,  Victor A. Torti

IPC: B05D300

CPC classification number: B01L3/5453

Abstract: A test tube comprises a tube body of unitary construction including an enclosed sidewall and an integral bottom that together define a tubular container having an open top. The bottom has a concave interior surface and a planar exterior surface upon which machine readable data is encoded within a multi-layered opaque coating that is deposited onto the planar exterior surface to uniquely identify the test tube. The machine readable data is preferably an open (i.e., non-proprietary) data matrix code. This code is applied to the test tube by depositing a multi-layer coating onto the planar exterior of the tube bottom. The multi-layer coating may include a first layer of opaque material that is deposited onto the planar exterior surface, and a second layer of opaque material that is deposited onto the first layer. The machine readable code is formed in the multi-layered coating by removing portions of the second layer.

Abstract translation: 试管包括整体构造的管体，其包括封闭侧壁和整体底部，所述底部共同限定具有开口顶部的管状容器。 底部具有凹入的内表面和平坦的外表面，机器可读数据被编码在多层不透明涂层内，该多层不透明涂层沉积在平面外表面上以唯一地识别试管。 机器可读数据优选地是开放（即非专有）数据矩阵代码。 该代码通过将多层涂层沉积到管底部的平面外部上而应用于试管。 多层涂层可以包括沉积在平面外表面上的第一不透明材料层和沉积在第一层上的第二不透明材料层。 通过去除第二层的部分，在多层涂层中形成机器可读代码。

公开(公告)号：US5858309A

公开(公告)日：1999-01-12

申请号：US621085

申请日：1996-03-22

Applicant: Gregory Mathus ,  Paul M. Szlosek ,  William J. Lacey

Inventor： Gregory Mathus ,  Paul M. Szlosek ,  William J. Lacey

IPC: G01N1/00 ,  B01L3/00 ,  B29C45/14 ,  B29D99/00 ,  B29K105/32 ,  B29L22/00 ,  B29L31/00 ,  B32B27/30 ,  C08L27/12 ,  C12M1/32 ,  G01N1/10 ,  G01N21/03 ,  G01N21/25 ,  G01N21/33

CPC classification number: B01L3/5085 ,  B29C45/1418 ,  B29C45/14344 ,  B29C45/14778 ,  G01N21/03 ,  G01N21/25 ,  G01N21/33 ,  B01L2300/0829 ,  B01L2300/12

Abstract: Microplates and methods for manufacturing microplates. The microplate is designed to allow UV radiation to pass through the bottom wells of the microplate so that the microplate can be used for assaying samples by use of UV absorbance. In one embodiment, the microplate comprises at least first and second wells, each well having a UV permeable bottom. In another embodiment, the microplate comprises a frame having an upper portion and a lower portion contiguous with the upper portion and a sheet disposed between the upper portion and the lower portion and defining the bottom of at least one well of the microplate. One embodiment of the method includes steps of inserting a sheet of UV permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, injecting molten plastic material into the mold cavity, and cooling the plastic material to form the microplate with the plastic material forming the sidewalls of each of the first and second wells and the sheet of UV permeable material forming the bottom of each of the first and second wells. Another embodiment of the method includes providing an upper plate defining sidewalls of at least one well, adhering an intermediate layer to the upper plate and adhering a sheet of UV permeable material to the intermediate layer. A further embodiment of the method includes inserting a sheet of material having at least one hole into a mold cavity, injecting a molten plastic material into the mold cavity and cooling the plastic material to form a microplate.

Abstract translation: 微孔板和微孔板制造方法。 微孔板被设计成允许紫外线辐射通过微板的底部孔，使得微孔板可以用于通过使用UV吸光度来测定样品。 在一个实施方案中，微板包括至少第一和第二孔，每个孔具有可UV透过的底部。 在另一个实施例中，微孔板包括具有与上部邻接的上部和下部的框架，以及设置在上部和下部之间并限定微板的至少一个孔的底部的框架。 该方法的一个实施例包括以下步骤：将一片UV可渗透材料插入到模腔中，该模腔包括成形为形成多个孔的侧壁的部分，将熔融的塑料材料注入模腔中，并冷却塑料材料以形成 微板与塑料材料形成第一和第二阱中的每一个的侧壁以及形成第一和第二孔中每一个的底部的UV透过材料片。 该方法的另一实施例包括提供限定至少一个阱的侧壁的上板，将中间层粘附到上板并将一片UV可渗透材料粘合到中间层。 该方法的另一实施例包括将具有至少一个孔的材料片插入模腔中，将熔融的塑料材料注入模腔并冷却塑料以形成微板。

公开(公告)号：US5139951A

公开(公告)日：1992-08-18

申请号：US595305

申请日：1990-10-10

Applicant: David Butz ,  George Lyman ,  David Root ,  Gregory Mathus

Inventor： David Butz ,  George Lyman ,  David Root ,  Gregory Mathus

IPC: C12M3/06

CPC classification number: C12M25/02 ,  C12M23/00 ,  C12M23/12

Abstract: A tissue or cell growth device is described for placement within a well of a cluster dish. The device has a cell or tissue retention element detachably attached to a hanger. The hanger preferably has openings allowing access to the well without removal of the member and is capable of positioning the cell or tissue retention element a preselected distance relative to the hanger. Also described are various embodiments of the device having differently shaped hangers and the combination of the device and a cluster dish.

Abstract translation: 描述了组织或细胞生长装置用于放置在簇盘的孔内。 该装置具有可拆卸地附接到衣架的细胞或组织保持元件。 衣架优选地具有允许进入井的开口，而不移除构件，并且能够将电池或组织保持元件相对于衣架定位成预选的距离。 还描述了具有不同形状的悬挂器以及装置和簇盘的组合的装置的各种实施例。

公开(公告)号：US5026649A

公开(公告)日：1991-06-25

申请号：US841562

申请日：1986-03-20

Applicant: George Lyman ,  Gregory Mathus ,  David Root

Inventor： George Lyman ,  Gregory Mathus ,  David Root

IPC: C12M1/20 ,  C12M1/22 ,  C12M3/00 ,  C12M3/06

CPC classification number: C12M29/04 ,  C12M25/04

Abstract: Apparatus for growing tissue cultures in vitro, which permits a concentration gradient of nutrients to develop through a permeable membrane to which a sample of tissue is attached. The permeable membrane is attached to the bottom end of a tubular support that in turn hangs by a flange connected to its upper end on the top of a well containing the nutrients. Typically, the well is part of a tissue culture cluster dish. The flange of the support positions the support and membrane centrally in the well so as to avoid capillary action in the space between the well and support. The configuration of the support and its cooperation with the lid of the cluster dish also prevents the support and membrane from floating in the nutrient solution in the well. Openings in the support provide access for a pipette to add and withdraw fluid from the space between the well and membrane support and from the space below the membrane.

Abstract translation: 用于在体外生长组织培养物的装置，其允许营养物的浓度梯度通过附着有组织样品的渗透膜发育。 可渗透膜附接到管状支撑件的底端，管状支撑件的底端又通过连接到容纳营养物质的孔的顶部上方的凸缘而悬挂。 通常，孔是组织培养簇盘的一部分。 支撑件的凸缘将支撑件和膜中心定位在井中，以避免在井和支撑件之间的空间中的毛细作用。 支架的配置及其与簇盘盖的配合也可防止支撑和膜浮在井中的营养液中。 支架中的开口提供移液管的通道，以从孔和膜支撑之间的空间以及膜下方的空间添加和抽出流体。

公开(公告)号：US4927764A

公开(公告)日：1990-05-22

申请号：US122052

申请日：1987-11-17

Applicant: George Lyman ,  Gregory Mathus

Inventor： George Lyman ,  Gregory Mathus

IPC: B01L3/08 ,  C12M1/24

CPC classification number: C12M23/32 ,  B01L3/08 ,  C12M23/08 ,  C12M23/22 ,  C12M23/38

Abstract: A tissue culture flask having a flat bottom wall with optical properties on the inner surface of which cells are grown. A top wall opposite the bottom wall has a large opening therethrough which provides access to the growing surface for the removal of cells growing on the surface. The opening is closed by a flexible transparent film which is sealed to the top wall and which may be peeled off the top wall to provide access to the flask interior.

Abstract translation: 一种组织培养瓶，其具有在其内表面上生长细胞的具有光学性质的平坦底壁。 与底壁相对的顶壁具有穿过其的大开口，其提供进入生长表面以去除在表面上生长的细胞。 开口由柔性透明膜封闭，该透明膜密封到顶壁，并且可以从顶壁剥离以提供通向烧瓶内部的通路。