公开(公告)号：US20170166957A1

公开(公告)日：2017-06-15

申请号：US15411482

申请日：2017-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

公开(公告)号：US09556475B2

公开(公告)日：2017-01-31

申请号：US14457863

申请日：2014-08-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract translation: 用于使用扩增报道子进行扩增测定的系统，包括方法，装置和组合物，所述扩增报告基团包括能够在报道分子的熔点以下彼此碱基配对的第一低聚物和第二低聚物。 报告物可能具有受到影响的可检测的光致发光，例如通过第一和第二寡聚体彼此的碱基配对而减少。 可以在高于熔解温度的至少一个体积（例如多个分区）中扩增靶，例如核酸靶序列，并且可以从低于熔解温度的至少一个体积检测报告物的光致发光 。 可以基于检测到的光致发光来确定靶的特性，例如靶的浓度。

公开(公告)号：US20160076099A1

公开(公告)日：2016-03-17

申请号：US14848311

申请日：2015-09-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Serge Saxonov ,  Michael Y. Lucero ,  Benjamin J. Hindson ,  Phillip Belgrader ,  Simant Dube ,  Austin P. So ,  Jeffrey C. Mellen ,  Nicholas J. Heredia ,  Kevin D. Ness ,  Billy W. Colston, Jr.

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/683 ,  C12Q1/6858 ,  C12Q2600/158 ,  C12Q2600/172 ,  C12Q2537/16 ,  C12Q2563/159 ,  C12Q2537/165

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract translation: 本文提供了用于分析核酸的改进的方法，组合物和试剂盒。 改进的方法，组合物和试剂盒可以使得样品中核酸的拷贝数估计可以进行。 本文还提供了用于确定样品中靶核酸的两个或多个拷贝（例如两个或多个拷贝是在同一染色体上还是在不同染色体上）或用于定相等位基因的连接的方法，组合物和试剂盒。

公开(公告)号：US20150148250A1

公开(公告)日：2015-05-28

申请号：US14457863

申请日：2014-08-12

Applicant: Bio-Rad laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract translation: 用于使用扩增报道子进行扩增测定的系统，包括方法，装置和组合物，所述扩增报告基团包括能够在报道分子的熔点以下彼此碱基配对的第一低聚物和第二低聚物。 报告物可能具有受到影响的可检测的光致发光，例如通过第一和第二寡聚体彼此的碱基配对而减少。 可以在高于熔解温度的至少一个体积（例如多个分区）中扩增靶，例如核酸靶序列，并且可以从低于熔解温度的至少一个体积检测报告物的光致发光 。 可以基于检测到的光致发光来确定靶的特性，例如靶的浓度。