公开(公告)号：US5457181A

公开(公告)日：1995-10-10

申请号：US683109

申请日：1991-04-11

申请人： Catherine Michalski ,  Thierry Burnouf

发明人： Catherine Michalski ,  Thierry Burnouf

IPC分类号： G01N33/48 ,  A61K35/14 ,  A61K38/00 ,  A61K38/43 ,  B01D15/08 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/30 ,  C07K14/00 ,  C07K14/745 ,  C12N9/64 ,  G01N30/88

CPC分类号： C12N9/647 ,  C07K14/745 ,  A61K38/00

摘要： The present invention relates to a method for preparing a high purity Factor IX concentrate. The starting material for the process is the supernatant fraction of a cryoprecipitated human plasma. A pre-purification step is performed by DEAE-Sephadex chromatography. The resulting Factor IX fraction has a specific activity of at least 0.5 IU/mg protein. The purification method of the invention comprises two successive chromatography separations. First, ion-exchange chromatography on DEAE-sepharose is conducted so that the Factor IX is eluted when the ionic force of the buffer is increased to 0.34-0.38M sodium chloride. Then, affinity chromatography is conducted on heparin-sepharose. The elution buffer is a citrate buffer at a pH of 7.4 adjusted with 0.45M sodium chloride and supplemented with arginine as a stabilizer for Factor IX activity. Lysine is added as a stabilizer before freeze-drying of the purified Factor IX.

摘要翻译： 本发明涉及制备高纯度因子IX浓缩物的方法。 该方法的起始原料是冷沉淀的人血浆的上清液部分。 通过DEAE-Sephadex色谱法进行预纯化步骤。 所得的因子IX级分具有至少0.5IU / mg蛋白质的比活性。 本发明的纯化方法包括两次连续的色谱分离。 首先，进行DEAE-琼脂糖凝胶上的离子交换层析，使得当缓冲液的离子力增加至0.34-0.38M氯化钠时，因子IX被洗脱。 然后，对肝素琼脂糖进行亲和层析。 洗脱缓冲液是用0.45M氯化钠调节pH 7.4的柠檬酸盐缓冲液，补充精氨酸作为因子IX活性的稳定剂。 加入赖氨酸作为稳定剂，然后冷冻干燥纯化的因子IX。

公开(公告)号：US20130143810A1

公开(公告)日：2013-06-06

申请号：US13684746

申请日：2012-11-26

申请人： Thierry BURNOUF ,  Cheng-Yao SU

发明人： Thierry BURNOUF ,  Cheng-Yao SU

IPC分类号： C12N5/00

CPC分类号： C12N5/0018 ,  A61K35/19 ,  A61K38/1808 ,  A61K38/1841 ,  A61K38/30 ,  A61K2300/00

摘要： The invention concerns human platelet extracts rich in growth factors (PGF) for wound healing and stem cell expansion. Accordingly the subject invention relates to a virally-inactivated growth factors-containing platelet lysate depleted of PDGF and VEGF, which is preferably enriched in TGF, IGF and EGF-rich. The present invention further concerns a method for obtaining a platelet lysate comprising the steps of contacting a starting platelet concentrate with a solvent and/or a detergent, incubating the starting platelet concentrate with the solvent and/or detergent for a period of at least 5 minutes to 6 hours, at a pH maintained in a range from about 6.0 to about 9.0, and at a temperature within the range of from 2° C. to 50° C., optionally removing the solvent and/or the detergent by oil extraction and obtaining an aqueous protein phase, and incubating the solvent and/or detergent-treated platelet concentrate or the aqueous protein phase with charcoal.

摘要翻译： 本发明涉及富含生长因子（PGF）的人血小板提取物用于伤口愈合和干细胞扩增。 因此，本发明涉及耗尽PDGF和VEGF的病毒灭活的含生长因子的血小板裂解物，其优选富含富含TGF，IGF和富含EGF的血小板裂解物。 本发明还涉及获得血小板裂解物的方法，其包括以下步骤：使起始血小板浓缩物与溶剂和/或洗涤剂接触，将起始血小板浓缩物与溶剂和/或洗涤剂一起孵育至少5分钟 至约6小时，pH保持在约6.0至约9.0，温度范围为2℃至50℃，任选地通过油提取除去溶剂和/或洗涤剂; 获得蛋白质水相，并将溶剂和/或洗涤剂处理的血小板浓缩物或水性蛋白质相与活性炭一起温育。

公开(公告)号：US20110027257A1

公开(公告)日：2011-02-03

申请号：US12744200

申请日：2009-01-07

申请人： Thierry Burnouf ,  Cheng-Yao Su

发明人： Thierry Burnouf ,  Cheng-Yao Su

IPC分类号： A61K38/48 ,  C07K14/49 ,  C07K14/495 ,  C07K14/485 ,  C07K14/65 ,  C07K14/50 ,  A61K38/18 ,  A61K38/30 ,  A61K38/22 ,  A61K38/39 ,  A61K8/64 ,  A61P17/02 ,  A61P19/00 ,  A61P9/00 ,  A61P7/02 ,  A61Q90/00

CPC分类号： A61K38/30 ,  A61K8/64 ,  A61K38/18 ,  A61K38/1808 ,  A61K38/1825 ,  A61K38/1841 ,  A61K38/1858 ,  A61K38/1866 ,  A61K38/39 ,  A61K2800/10 ,  A61Q19/00 ,  C07K14/49 ,  A61K2300/00

摘要： The present disclosure relates to a clottable concentrate of platelet growth factors for therapeutic and/or cosmetic use, preferably comprising the growth factors PDGF, TGT-β, IGF, EGF, CTGF, bFGF and VEGF. In a preferred embodiment, the clottable concentrate of platelet growth factors does not induce blood cell-related transfusion reactions. The present disclosure also relates to a method for preparing a clottable concentrate of platelet growth factors including the steps of contacting a platelet concentrate with a solvent and/or a detergent, incubating the platelet concentrate with the solvent and/or detergent for a period of at least 5 minutes to 6 hours, at a pH maintained in a range from about 6.0 to about 9.0, and at a temperature within the range of from 2° C. to 50° C., preferably within the range of from 25° C. to 45° C., and removing the solvent and/or the detergent by oil extraction and/or chromatographic means.

摘要翻译： 本公开内容涉及用于治疗和/或美容使用的可凝结的血小板生长因子浓缩物，优选包含生长因子PDGF，TGT-和bgr; IGF，EGF，CTGF，bFGF和VEGF。 在优选的实施方案中，血小板生长因子的可凝结浓缩物不诱导与血细胞相关的输血反应。 本公开还涉及一种制备血小板生长因子的可凝结浓缩物的方法，包括以下步骤：使血小板浓缩物与溶剂和/或洗涤剂接触，将血小板浓缩物与溶剂和/或洗涤剂一起温育一段时间 至少5分钟至6小时，pH值范围为约6.0至约9.0，温度范围为2℃至50℃，优选为25℃。 至45℃，并通过油提取和/或色谱法除去溶剂和/或洗涤剂。

公开(公告)号：US6022954A

公开(公告)日：2000-02-08

申请号：US774745

申请日：1991-10-11

申请人： Dominique Dernis ,  Thierry Burnouf

发明人： Dominique Dernis ,  Thierry Burnouf

IPC分类号： C07K14/435 ,  A61K38/38 ,  C07K1/14 ,  C07K1/18 ,  C07K1/36 ,  C07K14/76 ,  C07K14/765 ,  C12N5/02 ,  A61K38/00 ,  C07G7/00

CPC分类号： C07K14/76

摘要： The invention relates to a process for preparing purified albumin from a human or animal physiological solution, such as a plasma or a plasma fraction. The process includes a process of delipidation using an anionic detergent and two chromatographic separation stages using ion-exchange resin. By applying the process according to the invention, it is possible to obtain an albumin solution of great purity, that is stable and suitable for therapeutic use.

摘要翻译： 本发明涉及从人或动物生理溶液如血浆或血浆级分制备纯化白蛋白的方法。 该方法包括使用离子交换树脂使用阴离子洗涤剂和两个色谱分离阶段脱脂的方法。 通过应用本发明的方法，可以获得稳定且适合治疗用途的纯度高的白蛋白溶液。

公开(公告)号：US5252709A

公开(公告)日：1993-10-12

申请号：US460972

申请日：1990-04-06

申请人： Thierry Burnouf ,  Miryana Burnouf-Radosevich

发明人： Thierry Burnouf ,  Miryana Burnouf-Radosevich

IPC分类号： A61K38/16 ,  A61K35/16 ,  A61K38/43 ,  C07K1/18 ,  C07K14/435 ,  C07K14/745 ,  C07K14/755 ,  C07K14/78 ,  C07K3/22 ,  C07K15/06

CPC分类号： C07K14/755

摘要： The invention relates to a process for separating proteins from a fraction of human or animal plasma.According to this process, a solubilized fraction of cryoprecipitated plasma is subjected to a single stage of chromatography on a moderately ionic anion exchange resin permitting hydrophobic interactions to take place, which does not adsorb certain proteins and fixes others, which are then eluted by increasing the ionic strength of the buffer by the addition of NaCl.The process according to the invention makes it possible, in particular, to obtain a Factor VIII concentrate of high purity that can be used for the treatment of haemophilia A. The process also makes it possible to obtain concentrates of fibrinogen, Von Willebrand's factor and fibronectin.

摘要翻译： PCT No.PCT / FR89 / 00050 Sec。 371日期：1990年4月6日 102（e）日期1990年4月6日PCT提交1989年2月8日PCT公布。 出版物WO89 / 12065 日本1989年12月14日。本发明涉及从人或动物血浆部分中分离蛋白质的方法。 根据该方法，将冷沉淀的等离子体的溶解部分在中等离子阴离子交换树脂上进行单层色谱，允许发生疏水相互作用，其不吸附某些蛋白质并固定其它蛋白质，然后通过增加 通过加入NaCl的缓冲液的离子强度。 根据本发明的方法使得特别可以获得可用于治疗血友病A的高纯度因子VIII浓缩物。该方法还使得可以获得纤维蛋白原浓度，Von Willebrand因子和纤连蛋白 。