公开(公告)号：US08394610B2

公开(公告)日：2013-03-12

申请号：US12388568

申请日：2009-02-19

申请人： Andrey Yurievich Gulevich ,  Aleksandra Yurievna Skorokhodova ,  Vladimir Yurievich Ermishev ,  Natalya Igorevna Minaeva ,  Danila Vadimovich Zimenkov ,  Aleksandr Aleksandrovich Krylov ,  Irina Vladimirovna Biryukova ,  Sergei Vladimirovich Mashko

发明人： Andrey Yurievich Gulevich ,  Aleksandra Yurievna Skorokhodova ,  Vladimir Yurievich Ermishev ,  Natalya Igorevna Minaeva ,  Danila Vadimovich Zimenkov ,  Aleksandr Aleksandrovich Krylov ,  Irina Vladimirovna Biryukova ,  Sergei Vladimirovich Mashko

IPC分类号： C12N15/64 ,  C07H21/04

CPC分类号： C12N15/70

摘要： The present invention provides a method for constructing recombinant translationally coupled operons, a method for producing useful metabolites using the bacterium containing the coupled operons, and a method for monitoring gene expression.

摘要翻译： 本发明提供构建重组翻译偶联的操纵子的方法，使用含有偶联的操纵子的细菌产生有用的代谢物的方法，以及监测基因表达的方法。

公开(公告)号：US20120219996A1

公开(公告)日：2012-08-30

申请号：US13466266

申请日：2012-05-08

申请人： Konstantin Vyacheslavovich Rybak ,  Ekaterina Aleksandrovna Slivinskaya ,  Ekaterina Alekseevna Savrasova ,  Valeriy Zavenovich Akhverdian ,  Elena Vitalievna Klyachko ,  Sergei Vladimirovich Mashko ,  Vera Georgievna Doroshenko ,  Larisa Gotlibovna Airikh ,  Tatyana Viktorovna Leonova ,  Mikhail Markovich Gusyatiner ,  Elvira Borisovna Voroshilova ,  Yury Ivanovich Kozlov ,  Yoshihiko Hara ,  Takuji Ueda

发明人： Konstantin Vyacheslavovich Rybak ,  Ekaterina Aleksandrovna Slivinskaya ,  Ekaterina Alekseevna Savrasova ,  Valeriy Zavenovich Akhverdian ,  Elena Vitalievna Klyachko ,  Sergei Vladimirovich Mashko ,  Vera Georgievna Doroshenko ,  Larisa Gotlibovna Airikh ,  Tatyana Viktorovna Leonova ,  Mikhail Markovich Gusyatiner ,  Elvira Borisovna Voroshilova ,  Yury Ivanovich Kozlov ,  Yoshihiko Hara ,  Takuji Ueda

IPC分类号： C12P13/08 ,  C12P13/04

CPC分类号： C12P13/04 ,  C12P13/08 ,  C12P13/10 ,  C12P13/24

摘要： There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

摘要翻译： 公开了使用肠杆菌科的细菌生产L-氨基酸的方法，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸或L-谷氨酸，其中细菌 已被修饰以增强D-木糖通透酶的活性。

公开(公告)号：US07915018B2

公开(公告)日：2011-03-29

申请号：US11247138

申请日：2005-10-12

申请人： Konstantin Vyacheslavovich Rybak ,  Ekaterina Aleksandrovna Slivinskaya ,  Ekaterina Alekseevna Savrasova ,  Valeriy Zavenovich Akhverdian ,  Elena Vitalievna Klyachko ,  Sergei Vladimirovich Mashko ,  Vera Georgievna Doroshenko ,  Larisa Gotlibovna Airikh ,  Tatyana Viktorovna Leonova ,  Mikhail Markovich Gusyatiner ,  Elvira Borisovna Voroshilova ,  Yury Ivanovich Kozlov ,  Yoshihiko Hara ,  Takuji Ueda

发明人： Konstantin Vyacheslavovich Rybak ,  Ekaterina Aleksandrovna Slivinskaya ,  Ekaterina Alekseevna Savrasova ,  Valeriy Zavenovich Akhverdian ,  Elena Vitalievna Klyachko ,  Sergei Vladimirovich Mashko ,  Vera Georgievna Doroshenko ,  Larisa Gotlibovna Airikh ,  Tatyana Viktorovna Leonova ,  Mikhail Markovich Gusyatiner ,  Elvira Borisovna Voroshilova ,  Yury Ivanovich Kozlov ,  Yoshihiko Hara ,  Takuji Ueda

IPC分类号： C12P13/08

CPC分类号： C12P13/04 ,  C12P13/08 ,  C12P13/10 ,  C12P13/24

摘要： There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

摘要翻译： 公开了使用肠杆菌科的细菌生产L-氨基酸的方法，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸或L-谷氨酸，其中细菌 已被修饰以增强D-木糖通透酶的活性。

公开(公告)号：US07604979B2

公开(公告)日：2009-10-20

申请号：US11222983

申请日：2005-09-12

申请人： Joanna Yosifovna Katashkina ,  Maria Grigorievna Lunts ,  Vera Georgievna Doroshenko ,  Svetlana Aleksandrovna Fomina ,  Aleksandra Yurievna Skorokhodova ,  Lirina Valerievna Ivanovskaya ,  Sergei Vladimirovich Mashko

发明人： Joanna Yosifovna Katashkina ,  Maria Grigorievna Lunts ,  Vera Georgievna Doroshenko ,  Svetlana Aleksandrovna Fomina ,  Aleksandra Yurievna Skorokhodova ,  Lirina Valerievna Ivanovskaya ,  Sergei Vladimirovich Mashko

IPC分类号： C12N1/20 ,  C12N15/11

CPC分类号： C12P13/04 ,  C12N9/0008 ,  C12P13/06 ,  C12P13/10 ,  C12P13/14 ,  C12P13/24

摘要： A method is provided for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genus Escherichia with an optimized level of expression of the gene which influences the distribution of carbon flow, such as the sucAB genes, comprising introducing into the chromosome of the bacterium a set of in vitro constructed DNA fragments which contain regulatory elements for gene expression instead of the native elements of the regulatory region of the gene, and selecting the colonies with increased L-amino acid productivity. Also, a method is provided for producing an L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with an optimized level of expression of the sucAB gene.

摘要翻译： 提供一种获得属于埃希氏杆菌属的L-氨基酸或产生核酸的细菌的方法，其具有影响碳流分布的基因表达的最佳水平，例如sucAB基因，包括引入染色体 的细菌一组体外构建的DNA片段，其含有用于基因表达的调节元件，而不是基因调节区的天然元件，并选择具有增加的L-氨基酸生产力的菌落。 此外，提供了使用具有优化的sucAB基因表达水平的细菌来生产L-氨基酸的方法，例如L-谷氨酸，L-脯氨酸，L-精氨酸，L-谷氨酰胺，L-亮氨酸 。

公开(公告)号：US20090191601A1

公开(公告)日：2009-07-30

申请号：US12372060

申请日：2009-02-17

申请人： Andrey Yurievich Gulevich ,  Tatyana Aleksandrovna Gaydenko ,  Vladimir Yurievich Ermishev ,  Aleksandra Yurievna Skorokhodova ,  Irina Vladimirovna Biryukova ,  Sergei Vladimirovich Mashko

发明人： Andrey Yurievich Gulevich ,  Tatyana Aleksandrovna Gaydenko ,  Vladimir Yurievich Ermishev ,  Aleksandra Yurievna Skorokhodova ,  Irina Vladimirovna Biryukova ,  Sergei Vladimirovich Mashko

IPC分类号： C12P13/22 ,  C12N1/21 ,  C12P13/04

CPC分类号： C12P13/222 ,  C07K14/245 ,  C12N9/0006 ,  C12P13/225 ,  C12P13/227 ,  C12P21/02

摘要： The present invention provides a method for producing an aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ydiN gene, the ydiB gene, or both.

摘要翻译： 本发明提供一种使用肠杆菌科的细菌，特别是属于埃希氏杆菌属或泛菌属的细菌生产芳香族L-氨基酸的方法，其已经被修饰以减弱ydiN基因，ydiB基因或两者的表达 。