公开(公告)号：US20140227270A1

公开(公告)日：2014-08-14

申请号：US14008011

申请日：2012-03-28

申请人： Francis Descamps ,  David André Baptiste Maussang-Detaille ,  Maarten Van Roy ,  Regorius Leurs ,  Maria Gonzalez Pajuelo ,  Pascal Gerard Merchiers ,  Martine Smit ,  Catelijne Stortelers ,  Philippe Van Rompaey ,  Peter Vanlandschoot

发明人： Francis Descamps ,  David André Baptiste Maussang-Detaille ,  Maarten Van Roy ,  Regorius Leurs ,  Maria Gonzalez Pajuelo ,  Pascal Gerard Merchiers ,  Martine Smit ,  Catelijne Stortelers ,  Philippe Van Rompaey ,  Peter Vanlandschoot

IPC分类号： C07K16/28

CPC分类号： C07K16/2866 ,  A61K2039/507 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2319/31

摘要： The present invention relates to particular polypeptides, nucleic acids encoding such polypeptides; to methods for preparing such polypeptides; to host cells expressing or capable of expressing such polypeptides; to compositions and in particular to pharmaceutical compositions that comprise such polypeptides, for prophylactic, therapeutic or diagnostic purposes. In particular, the present invention provides immunoglobulin single variable domains inhibiting CXCR7 mediated tumour growth.

摘要翻译： 本发明涉及特定的多肽，编码这种多肽的核酸; 制备这种多肽的方法; 以宿主表达或能够表达这种多肽的细胞; 组合物，特别是涉及用于预防，治疗或诊断目的的包含这种多肽的药物组合物。 特别地，本发明提供了抑制CXCR7介导的肿瘤生长的免疫球蛋白单可变结构域。

公开(公告)号：US20120128669A1

公开(公告)日：2012-05-24

申请号：US13375357

申请日：2010-06-07

申请人： Erik Depla ,  Catelijne Stortelers ,  Stephanie Staelens

发明人： Erik Depla ,  Catelijne Stortelers ,  Stephanie Staelens

IPC分类号： A61K39/42 ,  C12N15/13 ,  C12N15/63 ,  C12P21/02 ,  C07K1/107 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  A01H5/00 ,  A61P31/14 ,  A61P11/00 ,  A61P29/00 ,  A61P11/06 ,  C07K16/10

CPC分类号： C07K16/1027 ,  A61K2039/505 ,  A61M11/00 ,  A61M15/0065 ,  A61M2202/064 ,  C07K2317/22 ,  C07K2317/34 ,  C07K2317/35 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/626 ,  C07K2317/76 ,  C07K2317/92

摘要： Amino acid sequences are provided that are directed against and/or that can specifically bind protein F of hRSV, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The amino acid sequences, polypeptides and therapeutic compounds and compositions provided by the invention show an improved stability, less immunogenicity and/or improved affinity and/or avidity for protein F of hRSV. The invention also relates to the uses of such amino acid sequences, polypeptides, compounds or constructs for prophylactic and/or therapeutic purposes.

摘要翻译： 提供了针对和/或可以特异性结合hRSV的蛋白F的氨基酸序列，以及包含或基本上由一个或多个这样的氨基酸序列组成的化合物或构建体，特别是蛋白质和多肽。 由本发明提供的氨基酸序列，多肽和治疗化合物和组合物显示hRSV的蛋白F的改善的稳定性，较少的免疫原性和/或改善的亲和力和/或亲合力。 本发明还涉及这种氨基酸序列，多肽，化合物或构建体用于预防和/或治疗目的的用途。

公开(公告)号：US20110117113A1

公开(公告)日：2011-05-19

申请号：US12897051

申请日：2010-10-04

申请人： Gerald Beste ,  Stephanie Staelens ,  Peter Vanlandschoot ,  Maria Gonzalez Pajuelo ,  Hilde Adi Pierrette Revets ,  Peter Schotte ,  Hilde Stals ,  Ann Brige ,  Maarten Dewilde ,  Catelijne Stortelers

发明人： Gerald Beste ,  Stephanie Staelens ,  Peter Vanlandschoot ,  Maria Gonzalez Pajuelo ,  Hilde Adi Pierrette Revets ,  Peter Schotte ,  Hilde Stals ,  Ann Brige ,  Maarten Dewilde ,  Catelijne Stortelers

IPC分类号： A61K39/395 ,  C07K16/28 ,  C07K16/46 ,  C07K19/00 ,  A61P35/00 ,  A61P35/02 ,  A61P35/04 ,  A61P29/00 ,  A61P31/00 ,  A61P17/06 ,  A61P11/06 ,  A61P11/00 ,  A61P37/06

CPC分类号： C07K16/2866 ,  C07K16/00 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/92

摘要： The invention relates to immunoglobulin single variable domains directed against specific human CXCR4 epitopes (herein also referred to interchangeably as “compounds of the invention”, “amino acid sequences of the invention”, or “building blocks of the invention”) and polypeptides comprising them (herein also referred to as “polypeptides of the invention”, “compounds of the invention”, or “constructs of the invention”).Furthermore, the present invention relates to nucleic acids encoding the compounds of the invention (also referred to herein as “nucleic acids of the invention” or “nucleotide sequences of the invention”); to methods for preparing the compounds of the invention; to host cells expressing or capable of expressing the compounds of the invention; to compositions, and in particular to pharmaceutical compositions, that comprise the compounds of the invention; and to uses of the compounds of the invention and the aforementioned nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes, such as the prophylactic, therapeutic or diagnostic purposes mentioned herein.The invention also relates to methods for generating immunoglobulin single variable domains against a target such as a cell-associated protein and constructs comprising said immunoglobulin single variable domains. The invention also provides immunoglobulin single variable domains obtainable by the methods of the invention. Specifically, the present invention relates to the generation of immunoglobulin single variable domains and constructs thereof by use of epitope walking with multimer libraries. More specifically, the present invention relates to the generation of immunoglobulin single variable domains derived from camelids directed against a particular epitope of a target, in particular against a target with multiple transmembrane spanning domains, including GPCRs and ion channels, by epitope walking with multimer libraries.