公开(公告)号：US20090137414A1

公开(公告)日：2009-05-28

申请号：US11982467

申请日：2007-10-31

申请人： Radoje T. Drmanac ,  Matthew J. Callow ,  Snezana Drmanac ,  Brian K. Hauser ,  George Yeung

发明人： Radoje T. Drmanac ,  Matthew J. Callow ,  Snezana Drmanac ,  Brian K. Hauser ,  George Yeung

IPC分类号： C40B30/04 ,  C40B40/14 ,  C40B40/06 ,  C40B40/08 ,  C40B50/14

CPC分类号： C12Q1/6874 ,  C07H21/04 ,  C07K1/047 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2525/151 ,  C12Q2525/313 ,  C12Q2531/125 ,  C12Q2565/513 ,  G01N15/1404 ,  G01N15/1434 ,  Y10S977/778 ,  Y10S977/789 ,  Y10S977/792 ,  Y10S977/88 ,  Y10S977/882 ,  C12Q2521/307 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2563/179 ,  C12Q2565/514

摘要： Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.

公开(公告)号：US20110281738A1

公开(公告)日：2011-11-17

申请号：US13098965

申请日：2011-05-02

申请人： Radoje Drmanac ,  Matthew J. Callow ,  Brian K. Hauser ,  George Yeung

发明人： Radoje Drmanac ,  Matthew J. Callow ,  Brian K. Hauser ,  George Yeung

IPC分类号： C40B30/04 ,  C40B20/00

CPC分类号： C12Q1/6837 ,  C12N15/1093 ,  C12Q1/682 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/125 ,  C12Q2525/313 ,  C12Q2525/151 ,  C12Q2521/313 ,  C12Q2565/513 ,  C12Q2521/307

摘要： The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.

摘要翻译： 本发明提供制造和使用单个多核苷酸分子的自组装阵列进行各种大规模遗传测量的方法，例如基因表达分析，基因拷贝数评估等。 在本发明中使用的随机阵列是“自组装”的意思，即它们通过将多核苷酸分子沉积到其随机位置固定的表面上而形成。 然后通过直接序列测定组分核酸，例如并入的探针序列或其他解码方案来鉴定固定在表面上的多核苷酸分子。 这种鉴定将随机阵列的可测定多核苷酸及其各自的探针转换成可寻址的探针序列阵列。