公开(公告)号：US4945046A

公开(公告)日：1990-07-31

申请号：US57143

申请日：1987-06-03

申请人： Hajime Horii ,  Haruhide Kawabe ,  Hirofumi Arimura ,  Hiromichi Mukai ,  Kaoru Kobayashi ,  Muneo Tsujikawa ,  Masayuki Nishida ,  Tadakazu Suyama

发明人： Hajime Horii ,  Haruhide Kawabe ,  Hirofumi Arimura ,  Hiromichi Mukai ,  Kaoru Kobayashi ,  Muneo Tsujikawa ,  Masayuki Nishida ,  Tadakazu Suyama

IPC分类号： C07K14/02 ,  C12N15/81

CPC分类号： C07K14/005 ,  C12N15/81 ,  C12N2730/10122

摘要： A method of producing a heterologous protein in yeast is disclosed, which comprises transforming a yeast Saccharomyces cerevisiae with recombinant DNA comprising a promoter selected from the group consisting of a yeast promoter or a hybrid promoter derived from a yeast promoter, particularly a hybrid promoter containing the enhancer region of SV40 virus, and a gene coding for a heterologous protein such as HBsAg and Pre S-HBsAg, particularly full length Pre S-HBsAg culturing, the resulting transformed cells to express the gene coding the heterologous protein, and isolating the heterologous protein from the cultured medium. GAP-DH and PH05 promoters and miniaturized ones can be used as the yeast promoter. The expression of the full length Pre S-HBsAg is carried out in the same expression system as that for 2nd or 3rd Pre S-ABsAg.

摘要翻译： 公开了一种在酵母中产生异源蛋白质的方法，其包括用包含启动子的重组DNA转化酵母酿酒酵母，所述启动子选自酵母启动子或源自酵母启动子的杂合启动子，特别是含有 SV40病毒的增强子区和编码异源蛋白如HBsAg和Pre S-HBsAg的基因，特别是全长Pre S-HBsAg培养，所得转化细胞表达编码异源蛋白的基因，并分离异源蛋白 从培养基中。 GAP-DH和PH05启动子和小型化可用作酵母启动子。 全长前S-HBsAg的表达在与第2或第3前S-ABsAg相同的表达系统中进行。

公开(公告)号：US4904668A

公开(公告)日：1990-02-27

申请号：US101005

申请日：1987-09-25

申请人： Nobuo Kondo ,  Masahiro Kikuchi ,  Tsunetaka Nakajima ,  Masahiro Watanabe ,  Kazumasa Yokoyama ,  Takahiro Haga ,  Nobutoshi Yamada ,  Hideo Sugi ,  Toru Koyanagi

发明人： Nobuo Kondo ,  Masahiro Kikuchi ,  Tsunetaka Nakajima ,  Masahiro Watanabe ,  Kazumasa Yokoyama ,  Takahiro Haga ,  Nobutoshi Yamada ,  Hideo Sugi ,  Toru Koyanagi

IPC分类号： A61K9/14 ,  C07D213/64 ,  C07D213/643 ,  C07D237/14 ,  C07D239/34

CPC分类号： C07D213/643 ,  A61K9/14 ,  A61K9/145 ,  A61K9/146 ,  C07D237/14 ,  C07D239/34

摘要： A benzoyl urea compound having the formula: ##STR1## wherein X is a halogen atom, a nitro group or a trifluoromethyl group, provided that when Y is a nitro group, X is a halogen atom or a nitro group, Y is a hydrogen atom, a halogen atom, a nitro group or a trifluoromethyl group, Z.sub.1 is a halogen atom or a trifluoromethyl group, Z.sub.2 is a hydrogen atom or a halogen atom, and A is a .dbd.CH-- group or a nitrogen atom, or the formula: ##STR2## wherein each of X.sub.1 is X.sub.2 is a hydrogen atom, a halogen atom or a nitro group, provided that when Y is a nitro group, X.sub.1 is a hydrogen atom, Y is a hydrogen atom, a halogen atom, a nitro group or a trifluoromethyl group, and Z is a hydrogen atom, a halogen atom or a trifluoromethyl group, characterized in that its average particle size is not larger than 1 .mu.m.

摘要翻译： 具有下式的苯甲酰脲化合物：其中X是卤素原子，硝基或三氟甲基，条件是当Y是硝基时，X是卤原子或硝基，Y是 氢原子，卤素原子，硝基或三氟甲基，Z1为卤素原子或三氟甲基，Z2为氢原子或卤素原子，A为a-CH-基或氮原子，或 其中X 1为X 2的每一个为氢原子，卤原子或硝基的式：其中Y为硝基时，X 1为氢原子，Y为氢原子， 卤原子，硝基或三氟甲基，Z为氢原子，卤素原子或三氟甲基，其特征在于，其平均粒径为1μm以下。

公开(公告)号：US4721777A

公开(公告)日：1988-01-26

申请号：US778708

申请日：1985-09-23

申请人： Yahiro Uemura ,  Katuhiro Uriyu ,  Tsuyoshi Takahashi ,  Takashi Goto ,  Masahiro Funayama ,  Masayuki Nishida ,  Tadakazu Suyama

发明人： Yahiro Uemura ,  Katuhiro Uriyu ,  Tsuyoshi Takahashi ,  Takashi Goto ,  Masahiro Funayama ,  Masayuki Nishida ,  Tadakazu Suyama

IPC分类号： C07K16/00 ,  A61K39/395 ,  A61L2/00 ,  C07K14/435 ,  C12P21/00 ,  C12P21/08 ,  C07K3/12

CPC分类号： A61L2/0023 ,  A61K39/39591 ,  Y10S530/861 ,  Y10S530/863

摘要： Virus-contaminated immunoglobulin can be virus-inactivated by heating it in a substantially dry state at a temperature of 30.degree. to 100.degree. C. for a period of time sufficient for inactivating virus with maintaining original activity of the immunoglobulin. The addition of glycine, sodium chloride, sodium acetate, polyethylene glycol, albumin or mannitol enhances the effect and gives good solubility and good state to the solution of the virus-inactivated immunoglobulin.

摘要翻译： 病毒污染的免疫球蛋白可以通过在30至100℃的温度下以基本上干燥的状态加热病毒灭活一段足以使病毒灭活并维持免疫球蛋白原始活性的时间。 加入甘氨酸，氯化钠，乙酸钠，聚乙二醇，白蛋白或甘露醇可增强病毒灭活的免疫球蛋白的作用，并提供良好的溶解度和良好的状态。

公开(公告)号：US4713459A

公开(公告)日：1987-12-15

申请号：US830094

申请日：1986-02-18

申请人： Kazumasa Yokoyama ,  Chikara Fukaya ,  Yoshio Tsuda ,  Taizo Ono ,  Yoshio Arakawa ,  Tadakazu Suyama

发明人： Kazumasa Yokoyama ,  Chikara Fukaya ,  Yoshio Tsuda ,  Taizo Ono ,  Yoshio Arakawa ,  Tadakazu Suyama

IPC分类号： A01N1/02 ,  A61K9/00 ,  C07D209/30 ,  C07D209/44 ,  C07D209/52 ,  C07D215/18 ,  C07D217/22 ,  C07D221/04 ,  C25B3/08 ,  A61K31/47 ,  A61K31/40 ,  C07D209/10

CPC分类号： A61K9/0026 ,  A01N1/02 ,  A01N1/0226 ,  C07D209/30 ,  C07D209/44 ,  C07D209/52 ,  C07D215/18 ,  C07D217/22 ,  C07D221/04 ,  C25B3/08

摘要： A novel compound of the formula, ##STR1## wherein R denotes a lower perfluoroalkyl group, l is an integer of 3 or 4, n+m is an integer of 2 or 3, provided that n may be zero, and at least one of ring A and ring B may be substituted by a lower perfluoroalkyl group, prepared by reacting the corresponding perhydro compound with fluorine is useful as a material capable of carrying oxygen in an aqueous emulsion for lifesaving a patient suffering from massive hemorrhage and for preserving internal organs in transplantation.

公开(公告)号：US4701325A

公开(公告)日：1987-10-20

申请号：US580183

申请日：1984-02-15

申请人： Yasuo Ueda ,  Yoshio Kagitani ,  Shirou Komeda ,  Satoshi Funakoshi

发明人： Yasuo Ueda ,  Yoshio Kagitani ,  Shirou Komeda ,  Satoshi Funakoshi

IPC分类号： A61K33/04 ,  A61P43/00 ,  A61K31/55

CPC分类号： A61K33/04

摘要： An alkali metal hydrogensulfite or sulfite is effective for controlling a local injury such as inflammation and vein injury occurring on the injection of an antitumor benzodiazepine compound of the formula ##STR1## wherein R.sub.1 denotes hydrogen atom, or acyl, carbamyl, or alkoxycarbonyl group; R.sub.2 denotes hydrogen atom or acyl group; and R.sub.3 denotes sulfinic acid rest, SO.sub.2 X, or sulfonic acid rest, SO.sub.3 X, X meaning hydrogen, alkali metal or alkaline earth metal. An aqueous solution containing the both can be administered by injection.

摘要翻译： 碱式金属亚硫酸氢盐或亚硫酸盐可有效地控制局部损伤，例如在注射式IMA IMA的抗肿瘤苯并二氮杂化合物时发生的炎症和静脉损伤，其中R1表示氢原子，或酰基，氨基甲酰基或烷氧基羰基; R2表示氢原子或酰基; R3表示亚硫酸基，SO2X或磺酸基，SO3X，X表示氢，碱金属或碱土金属。 可以通过注射施用含有这两者的水溶液。

公开(公告)号：US4654337A

公开(公告)日：1987-03-31

申请号：US751880

申请日：1985-07-05

申请人： Kazumasa Yokoyama ,  Chikara Fukaya ,  Yoshio Tsuda ,  Taizo Ono ,  Yoshio Arakawa ,  Yoshihisa Inoue ,  Youichiro Naito ,  Tadakazu Suyama

发明人： Kazumasa Yokoyama ,  Chikara Fukaya ,  Yoshio Tsuda ,  Taizo Ono ,  Yoshio Arakawa ,  Yoshihisa Inoue ,  Youichiro Naito ,  Tadakazu Suyama

IPC分类号： A61K9/00 ,  C07D453/02 ,  C07D471/08 ,  C07D487/08 ,  C25B3/08 ,  C07D223/00 ,  A61K31/435

CPC分类号： A61K9/0026 ,  C07D453/02 ,  C07D471/08 ,  C07D487/08 ,  C25B3/08 ,  Y10S514/832 ,  Y10S514/833

摘要： A novel compound of the formula, ##STR1## wherein k is zero or an integer of 1 to 2, j is an integer of from 1 or 3, l and m are each zero or an integer of from 1 to 3, and n is an integer of from 1 to 4, the rings A, B and C being optionally substituted with at least one trifluoromethyl group or tetrafluoroethyl group is prepared by reacting the perhydro compound corresponding thereto with fluorine. The compound is useful as a material capable of carrying oxygen in an aqueous emulsion for lifesaving a patient suffering from massive hemorrhage and for preserving internal organs in transplantation.

公开(公告)号：US4624927A

公开(公告)日：1986-11-25

申请号：US600740

申请日：1984-04-16

申请人： Tsunekazu Fukushima ,  Mitsugu Fujii ,  Satoshi Funakoshi ,  Tadakazu Suyama

发明人： Tsunekazu Fukushima ,  Mitsugu Fujii ,  Satoshi Funakoshi ,  Tadakazu Suyama

IPC分类号： G01N33/53 ,  C12Q1/56 ,  G01N33/86 ,  G01N31/00 ,  G01N33/556

CPC分类号： G01N33/86 ,  G01N2333/9108 ,  G01N2333/91085 ,  Y10S436/808 ,  Y10T436/106664

摘要： A reagent for determination of human blood coagulation factor XIII for reversed passive hemagglutination reaction which comprises sensitized erythrocytes prepared by sensitizing animal erythrocytes with specific anti-human factor XIII antibody, and a kit using the reagent are disclosed.

摘要翻译： 公开了一种用于测定用于反向被动血凝反应的人血液凝固因子XIII的试剂，其包括通过用特异性抗人因子XIII抗体对动物红细胞进行敏化制备的致敏红细胞和使用该试剂的试剂盒。

公开(公告)号：US4591599A

公开(公告)日：1986-05-27

申请号：US670971

申请日：1984-11-13

申请人： Kazumasa Yokoyama ,  Chikara Fukaya ,  Yoshio Tsuda ,  Taizo Ono ,  Yoshio Arakawa ,  Tadakazu Suyama

发明人： Kazumasa Yokoyama ,  Chikara Fukaya ,  Yoshio Tsuda ,  Taizo Ono ,  Yoshio Arakawa ,  Tadakazu Suyama

IPC分类号： A61K9/00 ,  C07D455/02 ,  C07D471/04 ,  C07D487/04 ,  C25B3/08 ,  C07D487/06 ,  A61K31/40

CPC分类号： A61K9/0026 ,  C07D455/02 ,  C07D471/04 ,  C07D487/04 ,  C25B3/08

摘要： A perfluorobicyclo compound of the general formula: ##STR1## wherein either or both of Ring A and Ring B may optionally be substituted with lower perfluoroalkyl group (s) and m and n each represent 3 or 4.

摘要翻译： 一种具有下列通式的全氟二环化合物：其中环A和环B中的任一个或两者可以任选地被低级全氟烷基取代，m和n各自表示3或4。

公开(公告)号：US4587122A

公开(公告)日：1986-05-06

申请号：US726321

申请日：1985-04-23

申请人： Yoshio Kagitani ,  Yasuo Ueda ,  Kozi Munechika ,  Satoshi Morimoto ,  Shirou Komeda ,  Kenji Tanaka ,  Kazumasa Yokoyama

发明人： Yoshio Kagitani ,  Yasuo Ueda ,  Kozi Munechika ,  Satoshi Morimoto ,  Shirou Komeda ,  Kenji Tanaka ,  Kazumasa Yokoyama

IPC分类号： A61K47/48 ,  C08B37/02 ,  A61K31/72 ,  A61K35/16 ,  A61K37/04

CPC分类号： C08B37/0021 ,  A61K47/48292 ,  Y10S530/813

摘要： The drug such as antitumor agents or antibiotics can preferentially be carried to a morbid part, for example, injured tissue and tumor site by administering its complex with fibronectin combined through an oxidized dextran to repair the morbid part. The complex is prepared by reacting the drug, an oxidized dextran having a cleaved structure of aldohexopyranose ring, and fibronectin.

摘要翻译： 药物如抗肿瘤剂或抗生素可优先通过将其与通过氧化葡聚糖结合的纤维连接蛋白的复合物施用于病态部分，例如损伤的组织和肿瘤部位来修复病态部分。 该复合物通过使药物，具有解离结构的羟基己糖吡喃糖环的氧化葡聚糖与纤连蛋白反应来制备。

公开(公告)号：US4560556A

公开(公告)日：1985-12-24

申请号：US572750

申请日：1984-01-23

申请人： Yoshio Kagitani ,  Kenji Tanaka ,  Yasuo Ueda ,  Yusei Shiraga ,  Tunetaka Nakajima ,  Takuji Doi ,  Takao Ohmura ,  Satoshi Funakoshi ,  Tadakazu Suyama

发明人： Yoshio Kagitani ,  Kenji Tanaka ,  Yasuo Ueda ,  Yusei Shiraga ,  Tunetaka Nakajima ,  Takuji Doi ,  Takao Ohmura ,  Satoshi Funakoshi ,  Tadakazu Suyama

IPC分类号： C07K14/705 ,  A61K31/40 ,  A61K31/43 ,  A61K31/545 ,  A61K31/70 ,  A61K38/00 ,  A61K38/16 ,  A61K38/22 ,  A61K47/48 ,  C07K14/435 ,  C07K14/78 ,  C07K19/00 ,  A61K35/16 ,  A61K37/04

CPC分类号： C07K14/78 ,  A61K47/48292 ,  A61K38/00 ,  Y10S530/829 ,  Y10S530/83

摘要： Physiologically active substances such as antibiotics can preferentially be carried to a morbid part, for example, injured tissue and neoplastic cell proliferation site by administering its complex with fibronectin to repair the morbid part. The complex is prepared by the reaction between the physiologically active substance and the fibronectin with or without intervention of a protein cross-linking agent.

摘要翻译： 生理活性物质如抗生素可以优先通过给予其与纤连蛋白的复合物来修复病态部分而被携带到病态部分，例如损伤组织和肿瘤细胞增殖部位。 该复合物通过蛋白质交联剂的介导或不涉及生理活性物质与纤连蛋白之间的反应来制备。