公开(公告)号：US4693972A

公开(公告)日：1987-09-15

申请号：US571001

申请日：1984-01-16

申请人： James D. Mansour ,  Thomas H. Schulte ,  Vernon R. Neece

发明人： James D. Mansour ,  Thomas H. Schulte ,  Vernon R. Neece

IPC分类号： G01N33/52 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/44 ,  G01N21/76 ,  G01N21/77 ,  C12Q1/02 ,  C12Q1/06 ,  G01N21/64

CPC分类号： C12Q1/04 ,  C12Q2304/12

摘要： A method for rapid detection of microorganisms in a body fluid sample includes detecting the microorganisms after treatment of the sample with a lysing agent in order to dissolve sample components other than microorganisms, and staining with a fluorescent dye. The lysing agent may be part of a composition which includes a culture medium thereby providing simultaneous lysis and growth before staining. Detection is preferably accomplished by reliance on the fluorescence emitted by the dye having been properly excited by light energy.A composition suitable for use in the above-described method includes a growth medium and a lysing agent.

摘要翻译： 用于体液样品中微生物快速检测的方法包括用裂解剂检测样品处理后的微生物，以溶解微生​​物以外的样品成分，并用荧光染料染色。 裂解剂可以是组合物的一部分，其包括培养基，从而在染色之前同时进行裂解和生长。 优选通过依赖于由光能适当激发的染料发出的荧光来实现检测。 适用于上述方法的组合物包括生长培养基和裂解剂。

公开(公告)号：US5798221A

公开(公告)日：1998-08-25

申请号：US714140

申请日：1996-09-16

申请人： Poul Erik AEgidius

发明人： Poul Erik AEgidius

IPC分类号： C12Q1/04 ,  C12Q1/37 ,  A23C9/12 ,  C12Q1/02 ,  G01N33/53

CPC分类号： C12Q1/04 ,  C12Q2304/12 ,  G01N2333/21 ,  Y10S435/874 ,  Y10S435/876 ,  Y10S435/968 ,  Y10S436/80 ,  Y10T436/104165

摘要： A method is disclosed for conditioning samples (of e.g. milk or meat) containing fat globules and somatic cells and/or protein particles before they are subjected to fluorescence measurements in order to determine the bacterial content, as well as methods for performing the determination of bacterial content in such samples. The conditioning method involves the treatment of the samples with an ion-chelating agent, a proteolytic enzyme, detergent, and a bacteriologically specific fluorochrome such as ethidium bromide. Detergent is used in a concentration resulting in substantially no dissolution of the fat globules and the conditioned samples thus loses insignificant amounts of fat globules. The assessment of fluorescence is preferably performed in a conventional flow cytometer. As no separation of fat globules is necessary, the methods are simple and fast. The bacterial determinations have proved reliable when compared to standard methods.

摘要翻译： PCT No.PCT / DK95 / 00119 Sec。 371日期1996年9月16日 102（e）日期1996年9月16日PCT 1995年3月16日PCT PCT。 出版物WO95 / 25174 日期1995年9月21日公开了一种方法，用于调节含有脂肪球，体细胞和/或蛋白质颗粒的样品（例如牛奶或肉），然后进行荧光测量以确定细菌含量，以及方法 执行这些样品中细菌含量的测定。 调理方法包括用离子螯合剂，蛋白水解酶，洗涤剂和细菌学特异性荧光染料如溴化乙锭处理样品。 洗涤剂的使用浓度导致脂肪球基本上不溶解，并且调理的样品因此失去微量的脂肪球。 荧光的评估优选在常规流式细胞仪中进行。 由于不需要分离脂肪球，所以方法简便快捷。 与标准方法相比，细菌测定证明是可靠的。

公开(公告)号：US4596089A

公开(公告)日：1986-06-24

申请号：US553629

申请日：1983-11-21

申请人： Tao-Chiuh Hsu

发明人： Tao-Chiuh Hsu

IPC分类号： C12Q1/04 ,  G01N1/30 ,  A01G1/00

CPC分类号： C12Q1/04 ,  C12Q2304/12 ,  G01N2001/305

摘要： A method for the staining of the chromosomal material of plant cells so as to induce G-Bands, useful in plant genetic studies such as hydridization. G-Banding is induced by contacting living plant cells, such as those of plants of the family Gramineae, with actinomycin D, N-methyl-N'-nitroguanidine or ethidium bromide. More particularly, plant cells are treated with AMD and colchicine before fixation, prepared for viewing through a microscope by staining and then mounted on a glass slide.

摘要翻译： 用于染色植物细胞的染色体材料以诱导G-带的方法，其可用于植物遗传学研究如杂交。 通过使生物植物细胞（例如禾本科植物的植物细胞）与放线菌素D，N-甲基-N'-硝基胍或溴化乙锭接触来诱导G带。 更具体地，在固定之前用AMD和秋水仙碱处理植物细胞，通过染色准备用于通过显微镜观察，然后安装在载玻片上。

公开(公告)号：US4025393A

公开(公告)日：1977-05-24

申请号：US632244

申请日：1975-11-17

申请人： Tomas Hirschfeld

发明人： Tomas Hirschfeld

IPC分类号： C12M1/34 ,  C12Q1/04 ,  G01N21/64 ,  C12K1/04

CPC分类号： G01N21/6428 ,  C12M41/36 ,  C12Q1/04 ,  C12Q2304/12 ,  G01N21/6458

摘要： A system for identifying within a mixed population, a group of microorganisms replicating in a culture medium specific to that group, which culture medium includes a fluorescence inhibitor which the group incorporates in their nucleic acid upon replication.In one embodiment, a sample of the microorganism population is dyed with two different fluorescent dyes which are specific to nucleic acid, the fluorescent emissivity of one of the dyes being reduced or quenched by the presence of the inhibitor in the dyed nucleic acid, the fluorescent emissivity of the other of the dyes being unaffected by the presence of the inhibitor. The ratio of intensities of the fluorescent emission from the two dyes is independent of the total nucleic acid content of each microorganism, but is dependent upon the extent of incorporation of the inhibitor into the nucleic acid, so serves as a marker or identifier of a replicated organism.In another embodiment of the invention, the sample of the population is dyed with but the one quenchable dye and the dyed organisms are exposed to high intensity radiation which very rapidly bleaches the dye. From the bleaching characteristics such as the time required for the fluorescent emission to decay from its initial intensity to l/e, or from the ratio of the initial intensity of emission to the integrated emission during bleaching, one can determine independently of the total amount of nucleic acid, changes in the quantum efficiency of the dye caused by the incorporation of the inhibitor in the nucleic acid, thereby identifying replicating organisms.

摘要翻译： 用于在混合群体内识别在该组特异性的培养基中复制的一组微生物的系统，该培养基包括该组在复制时在其核酸中并入的荧光抑制剂。

公开(公告)号：US11714848B2

公开(公告)日：2023-08-01

申请号：US17600499

申请日：2020-12-01

申请人： ILLUMINA CAMBRIDGE LIMITED

发明人： Natalie Morrell ,  Andrew Slatter ,  Vicki Thomson

IPC分类号： G06F16/58 ,  G06V10/26 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6844 ,  G06T5/50 ,  C12Q1/6855 ,  C12Q1/6853

CPC分类号： G06F16/58 ,  B01L3/502761 ,  B01L7/52 ,  C12Q1/6844 ,  G06T5/50 ,  G06V10/26 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/0893 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q2304/10 ,  C12Q2304/12 ,  C12Q2304/13

摘要： In an example method, a series of time-based clustering images is generated for a plurality of library fragments from a genome sample. Each time-based clustering image in the series is sequentially generated. To generate each time-based clustering image in the series: i) a respective sample is introduced to a flow cell, the respective sample including contiguity preserved library fragments of the plurality of library fragments, wherein the contiguity preserved library fragments are attached to a solid support or are attached to each other; ii) the contiguity preserved library fragments are released from the solid support or from each other; iii) the contiguity preserved library fragments are amplified to generate a plurality of respective template strands; iv) the respective template strands are stained; and v) the respective template strands are imaged.

公开(公告)号：US5437980A

公开(公告)日：1995-08-01

申请号：US63870

申请日：1993-05-17

申请人： Richard P. Haugland

发明人： Richard P. Haugland

IPC分类号： C09B15/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/50

CPC分类号： G01N33/5005 ,  C09B15/00 ,  C12Q1/04 ,  C12Q1/68 ,  C12Q2304/12

摘要： The invention relates to use of fluorescent compounds of the formula: ##STR1## where R contains between 4 and about 10 carbons and is optionally saturated or unsaturated, and is linear or branched or contains an alicyclic or aromatic ring; and the symbol .PSI. depicts the presence of the counterion used to neutralize the positive charge on the dye. The fluorescent dye dissolved in a biologically compatible solution stains a wide variety of living cells with a red nucleic acid stain after brief incubation in low concentrations of dye, without the requirement of permeabilizing reagents. Detection of the fluorescence can be used alone or in combination with measurement of other markers or properties of the cells to identify, discriminate or sort viable cells.

摘要翻译： 本发明涉及下式荧光化合物的用途：其中R含有4至约10个碳，并且任选地是饱和或不饱和的，并且是直链或支链或含有脂环族或芳香环; 并且符号PSI描述了用于中和染料上的正电荷的抗衡离子的存在。 溶解在生物相容性溶液中的荧光染料在低浓度染料下短暂孵育后，以红色核酸染色剂污染多种活细胞，而不需要透化试剂。 荧光的检测可以单独使用或与其他标记或细胞特性的测定结合使用以鉴别，鉴别或分类活细胞。

公开(公告)号：US4717660A

公开(公告)日：1988-01-05

申请号：US573988

申请日：1984-01-26

申请人： Thomas H. Schulte

发明人： Thomas H. Schulte

IPC分类号： C12Q1/04 ,  C12Q1/24

CPC分类号： C12Q1/04 ,  C12Q2304/12

摘要： A method for the detection of microorganisms e.g. bacteria in a blood sample comprises staining the microorganisms with a fluorescent dye and observing the fluorescence emission of an expanded buffy coat. The expanded buffy coat is obtained by centrifuging the stained sample in a hematocrit tube containing a float which occupies most of the buffy coat volume. The expanded buffy coat further separates into sub-layers. Intracellular microorganisms congregate in and are detected in the granulocyte sub-layer. The method may be modified to assess phagocytic activity.

摘要翻译： 用于检测微生物的方法 血液样品中的细菌包括用荧光染料染色微生物并观察扩张的血沉棕黄层的荧光发射。 扩张的血沉棕黄层是通过将染色的样品离心在含有大部分血沉棕黄层体积的浮子的血细胞比容管中而获得的。 扩张的血沉棕黄层进一步分成亚层。 细胞内的微生物聚集在粒细胞亚层中并被检测。 可以修改该方法以评估吞噬活性。