公开(公告)号：US20180230546A1

公开(公告)日：2018-08-16

申请号：US15880007

申请日：2018-01-25

Applicant: Wisconsin Alumni Research Foundation

Inventor： Paul Gerald Ahlquist ,  Srikumar Sengupta ,  Johan Arie den Boon ,  Bill Sugden ,  Michael Abbott Newton

IPC: C12Q1/6886 ,  C12Q1/6809 ,  C12N15/113

CPC classification number: C12Q1/6886 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/11 ,  C12N2330/10 ,  C12Q1/6809 ,  C12Q2600/106 ,  C12Q2600/158 ,  C12Q2600/178 ,  C12Q2537/143 ,  C12Q2525/207

Abstract: This invention provides methods for amplifying, detecting, measuring, and identifying miRNAs from biological samples, particularly limited amounts of a biological sample. miRNAs that are differentially expressed in tumor samples and normal tissues are useful as cancer biomarkers for cancer diagnostics.

公开(公告)号：US20160319364A1

公开(公告)日：2016-11-03

申请号：US15013189

申请日：2016-07-19

Applicant: Wisconsin Alumni Research Foundation

Inventor： Paul Gerald Ahlquist ,  Srikumar Sengupta ,  Johan Arie den Boon ,  Bill Sugden ,  Michael Abbott Newton

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/11 ,  C12N2330/10 ,  C12Q1/6809 ,  C12Q2600/106 ,  C12Q2600/158 ,  C12Q2600/178 ,  C12Q2537/143 ,  C12Q2525/207

Abstract: This invention provides methods for amplifying, detecting, measuring, and identifying miRNAs from biological samples, particularly limited amounts of a biological sample. miRNAs that are differentially expressed in tumor samples and normal tissues are useful as cancer biomarkers for cancer diagnostics.

公开(公告)号：US11808701B2

公开(公告)日：2023-11-07

申请号：US16769883

申请日：2018-12-04

Applicant: Wisconsin Alumni Research Foundation

Inventor： David Charles Schwartz ,  Subhrangshu Nandi ,  Michael Abbott Newton

IPC: G06K9/00 ,  G01N21/64 ,  G16B30/00 ,  C12Q1/6818 ,  G02B21/16 ,  G02B21/36 ,  G06V20/69

CPC classification number: G01N21/6428 ,  C12Q1/6818 ,  G01N21/6458 ,  G02B21/16 ,  G02B21/365 ,  G06V20/693 ,  G06V20/695 ,  G06V20/698 ,  G16B30/00 ,  G01N2021/6439 ,  G06V2201/03

Abstract: Systems and methods for identifying sequence information from measurements made on single nucleic acid molecules are disclosed. The systems and methods can include binding portions of nucleic acid molecules with marker molecules, such as fluorescent molecules and/or intercalating molecules. The marker molecules provide a detectable signal that includes information about the underlying genomic information of the location on the nucleic acid molecule where a given marker molecule is bound. A profile of the detectable signal along a position of the nucleic acid is acquired for multiple different nucleic acid molecules. The PRIMR algorithm processes the data to provide a consensus profile from which a consensus underlying genomic information can be determined.