公开(公告)号：US20220288589A1

公开(公告)日：2022-09-15

申请号：US17556100

申请日：2021-12-20

Applicant: Wisconsin Alumni Research Foundation ,  University of Leiden ,  University of Chicago

Inventor： David Charles Schwartz ,  Kristy L. Kounovsky-Shafer ,  Juan Pablo Hernandez-Ortiz ,  Konstantinos Dimitrios Potamousis ,  Juan Jose De Pablo ,  Theo Odijk ,  Kyubong Jo

IPC: B01L3/00 ,  G01N27/447 ,  G01N33/487

Abstract: Devices, systems, and methods for en masse patterning of nucleic acid molecule structures are disclosed. The devices can include microchannels and nanoslits. The microchannels and nanoslits can be connected by parking chambers. The systems and methods can utilize the geometry of the devices in coordination with a voltage application routine to park nucleic acid molecules in the parking chambers and subsequently inject the nucleic acid molecules into the nanoslits. The methods can be utilized to present nucleic acid molecules in a fashion suitable for genomic analysis. The methods can also be utilized to provide size selection of the nucleic acid molecules.

公开(公告)号：US11808701B2

公开(公告)日：2023-11-07

申请号：US16769883

申请日：2018-12-04

Applicant: Wisconsin Alumni Research Foundation

Inventor： David Charles Schwartz ,  Subhrangshu Nandi ,  Michael Abbott Newton

IPC: G06K9/00 ,  G01N21/64 ,  G16B30/00 ,  C12Q1/6818 ,  G02B21/16 ,  G02B21/36 ,  G06V20/69

CPC classification number: G01N21/6428 ,  C12Q1/6818 ,  G01N21/6458 ,  G02B21/16 ,  G02B21/365 ,  G06V20/693 ,  G06V20/695 ,  G06V20/698 ,  G16B30/00 ,  G01N2021/6439 ,  G06V2201/03

Abstract: Systems and methods for identifying sequence information from measurements made on single nucleic acid molecules are disclosed. The systems and methods can include binding portions of nucleic acid molecules with marker molecules, such as fluorescent molecules and/or intercalating molecules. The marker molecules provide a detectable signal that includes information about the underlying genomic information of the location on the nucleic acid molecule where a given marker molecule is bound. A profile of the detectable signal along a position of the nucleic acid is acquired for multiple different nucleic acid molecules. The PRIMR algorithm processes the data to provide a consensus profile from which a consensus underlying genomic information can be determined.

公开(公告)号：US20200078789A1

公开(公告)日：2020-03-12

申请号：US16614003

申请日：2018-05-16

Applicant: Wisconsin Alumni Research Foundation ,  University of Leiden ,  University of Chicago

Inventor： David Charles Schwartz ,  Kristy L. Kounovsky-Shafer ,  Juan Pablo Hernandez-Ortiz ,  Konstantinos Dimitrios Potamousis ,  Juan Jose De Pablo ,  Theo Odijk

IPC: B01L3/00 ,  G01N27/447 ,  G01N33/487

Abstract: Devices, systems, and methods for en masse patterning of nucleic acid molecule structures are disclosed. The devices can include microchannels and nanoslits. The microchannels and nanoslits can be connected by parking chambers. The systems and methods can utilize the geometry of the devices in coordination with a voltage application routine to park nucleic acid molecules in the parking chambers and subsequently inject the nucleic acid molecules into the nanoslits. The methods can be utilized to present nucleic acid molecules in a fashion suitable for genomic analysis. The methods can also be utilized to provide size selection of the nucleic acid molecules.

公开(公告)号：US11779922B2

公开(公告)日：2023-10-10

申请号：US17556100

申请日：2021-12-20

Applicant: Wisconsin Alumni Research Foundation ,  University of Leiden ,  University of Chicago

Inventor： David Charles Schwartz ,  Kristy L. Kounovsky-Shafer ,  Juan Pablo Hernandez-Ortiz ,  Konstantinos Dimitrios Potamousis ,  Juan Jose De Pablo ,  Theo Odijk ,  Kyubong Jo

IPC: B01L3/00 ,  G01N27/447 ,  G01N33/487

CPC classification number: B01L3/502761 ,  G01N27/44791 ,  G01N33/48721 ,  B01L2200/0663 ,  B01L2300/0645 ,  B01L2300/0896

Abstract: Devices, systems, and methods for en masse patterning of nucleic acid molecule structures are disclosed. The devices can include microchannels and nanoslits. The microchannels and nanoslits can be connected by parking chambers. The systems and methods can utilize the geometry of the devices in coordination with a voltage application routine to park nucleic acid molecules in the parking chambers and subsequently inject the nucleic acid molecules into the nanoslits. The methods can be utilized to present nucleic acid molecules in a fashion suitable for genomic analysis. The methods can also be utilized to provide size selection of the nucleic acid molecules.

公开(公告)号：US11213825B2

公开(公告)日：2022-01-04

申请号：US16614003

申请日：2018-05-16

Applicant: Wisconsin Alumni Research Foundation ,  University of Leiden ,  University of Chicago

Inventor： David Charles Schwartz ,  Kristy L. Kounovsky-Shafer ,  Juan Pablo Hernandez-Ortiz ,  Konstantinos Dimitrios Potamousis ,  Juan Jose De Pablo ,  Theo Odijk ,  Kyubong Jo

IPC: B01L3/00 ,  G01N27/447 ,  G01N33/487

Abstract: Devices, systems, and methods for en masse patterning of nucleic acid molecule structures are disclosed. The devices can include microchannels and nanoslits. The microchannels and nanoslits can be connected by parking chambers. The systems and methods can utilize the geometry of the devices in coordination with a voltage application routine to park nucleic acid molecules in the parking chambers and subsequently inject the nucleic acid molecules into the nanoslits. The methods can be utilized to present nucleic acid molecules in a fashion suitable for genomic analysis. The methods can also be utilized to provide size selection of the nucleic acid molecules.