公开(公告)号：US20220026365A1

公开(公告)日：2022-01-27

申请号：US17378479

申请日：2021-07-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark F. Oldham ,  Eric S. Nordman ,  Hongye Sun ,  Steven Boege

IPC: G01N21/64

Abstract: A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

公开(公告)号：US10597642B2

公开(公告)日：2020-03-24

申请号：US15690945

申请日：2017-08-30

Applicant: Life Technologies Corporation

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6804 ,  C12Q1/6818

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US09765310B2

公开(公告)日：2017-09-19

申请号：US14991230

申请日：2016-01-08

Applicant: Life Technologies Corporation

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US11447756B2

公开(公告)日：2022-09-20

申请号：US16825788

申请日：2020-03-20

Applicant: Life Technologies Corporation

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6804 ,  C12Q1/6818

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US09255258B2

公开(公告)日：2016-02-09

申请号：US14108166

申请日：2013-12-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

Abstract translation: 本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。 该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。 该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。 标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。 所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

公开(公告)号：US20250163391A1

公开(公告)日：2025-05-22

申请号：US18952663

申请日：2024-11-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12N9/12 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US11092548B2

公开(公告)日：2021-08-17

申请号：US16155831

申请日：2018-10-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark F. Oldham ,  Eric S. Nordman ,  Hongye Sun ,  Steven Boege

IPC: G01N21/00 ,  G01N21/64

Abstract: A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

公开(公告)号：US20160208318A1

公开(公告)日：2016-07-21

申请号：US14991230

申请日：2016-01-08

Applicant: Life Technologies Corporation

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

Abstract translation: 本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。 该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。 该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。 标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。 所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

公开(公告)号：US10107758B2

公开(公告)日：2018-10-23

申请号：US14323203

申请日：2014-07-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark F. Oldham ,  Eric S. Nordman ,  Hongye Sun ,  Steven J. Boege

IPC: G01N21/64

Abstract: A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

公开(公告)号：US09528152B2

公开(公告)日：2016-12-27

申请号：US14557753

申请日：2014-12-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Hongye Sun ,  Mark F. Oldham ,  John O'Neill ,  Charles R. Connell ,  Umberto Ulmanella ,  Aldrich N. K. Lau ,  Theo Kotseroglou ,  Kenneth J. Livak

IPC: C12Q1/68 ,  G01N27/414 ,  G01N33/543 ,  B01L3/00

CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  G01N27/3278 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/631

Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.