公开(公告)号：US11768198B2

公开(公告)日：2023-09-26

申请号：US16795467

申请日：2020-02-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: G01N33/53 ,  G01N33/532 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12N15/10

CPC classification number: G01N33/53 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/5436 ,  G01N33/58 ,  C12Q2521/10 ,  C12Q2563/179 ,  C12Q2565/30 ,  G01N2458/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20220220532A1

公开(公告)日：2022-07-14

申请号：US17700373

申请日：2022-03-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/6806 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12N15/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US12140590B2

公开(公告)日：2024-11-12

申请号：US17529082

申请日：2021-11-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: G01N33/53 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20220064711A1

公开(公告)日：2022-03-03

申请号：US17529082

申请日：2021-11-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/6806 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12N15/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20200318157A1

公开(公告)日：2020-10-08

申请号：US16838800

申请日：2020-04-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John Brian Hutchison ,  Jeffrey Charles Olson ,  Darren Roy Link

IPC: C12Q1/686

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.

公开(公告)号：US11965877B2

公开(公告)日：2024-04-23

申请号：US14874553

申请日：2015-10-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: G01N33/53 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58

CPC classification number: G01N33/53 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/5436 ,  G01N33/58 ,  C12Q2521/10 ,  C12Q2563/179 ,  C12Q2565/30 ,  G01N2458/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US11168353B2

公开(公告)日：2021-11-09

申请号：US16400858

申请日：2019-05-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12N15/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US12140591B2

公开(公告)日：2024-11-12

申请号：US17700373

申请日：2022-03-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C40B50/08 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  G01N33/53 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20240011974A1

公开(公告)日：2024-01-11

申请号：US18359296

申请日：2023-07-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: G01N33/58

CPC classification number: G01N33/58 ,  G01N2458/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20230220447A1

公开(公告)日：2023-07-13

申请号：US18178121

申请日：2023-03-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6804 ,  G01N33/543 ,  G01N33/58 ,  C40B50/08

CPC classification number: C12Q1/6806 ,  C12Q1/6874 ,  C12N15/1075 ,  C12Q1/6804 ,  G01N33/5436 ,  G01N33/58 ,  C40B50/08 ,  C12Q2521/10 ,  C12Q2565/30 ,  G01N2458/10 ,  C12Q2563/179

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.