公开(公告)号：US20080160496A1

公开(公告)日：2008-07-03

申请号：US11884767

申请日：2006-02-22

申请人： Victor Rzepakovsky ,  Ginadi Shaham ,  Udi Damari ,  Sachi Norman ,  Peter Bartal

发明人： Victor Rzepakovsky ,  Ginadi Shaham ,  Udi Damari ,  Sachi Norman ,  Peter Bartal

IPC分类号： A01N1/02 ,  A61F2/02 ,  A61B17/32 ,  A61B19/00

CPC分类号： A61F2/28 ,  A01N1/02 ,  A01N1/0242 ,  A61F2/30756 ,  A61F2/3094 ,  A61F2/4644 ,  A61F2002/30224 ,  A61F2002/3082 ,  A61F2002/30827 ,  A61F2230/0069

摘要： The present invention provides methods for providing cartilage-containing tissue for grafting, comprising providing excised cartilage-containing tissue; and treating said excised cartilage-containing and cryogenically preserving the treated cartilage-containing tissue under appropriate cryogenic preservation conditions so as to yield cryogenically preserved cartilage-containing tissue having at least 10% viable chondrocytes throughout the cartilage portion of the cartilage-containing tissue after preservation, as tested in a live/dead ratio assay. Treatment may comprise providing one or a plurality of incisions in said cartilage portion to a predetermined depth therein and/or introducing a cryoprotectant agent at least into said cartilage portion. The invention also provides viable cartilage obtainable by the methods of the invention, methods of grafting such preserved, viable cartilage containing tissue in a recipient, as well as apparatuses, vessels and systems for preparing a cartilage-containing tissue for cryogenic preservation and subsequent grafting in a recipient.

摘要翻译： 本发明提供用于提供用于移植的含软骨组织的方法，包括提供含有切除软骨的组织; 并在适当的低温保存条件下处理所述含有切割的含软骨和低温保存含有软骨的组织，以便在保存后的软骨含有组织的整个软骨部分中产生具有至少10％存活软骨细胞的低温保存的软骨组织 ，如在活/死比例测定中测试的。 治疗可以包括在所述软骨部分中提供一个或多个切口至其中的预定深度和/或至少将冷冻保护剂引入所述​​软骨部分。 本发明还提供了可通过本发明的方法获得的可行的软骨，在接受者中移植含有这种保存的，存活的软骨的组织的方法，以及用于制备用于低温保存的软骨组织并随后接枝的装置，血管和系统 收件人

公开(公告)号：US20100281886A1

公开(公告)日：2010-11-11

申请号：US12440846

申请日：2007-09-11

申请人： Ginadi Shaham ,  Yehudit Natan ,  Dana Lorber ,  Peter Bartal ,  Yoram Amsalem ,  Yaniv Damari

发明人： Ginadi Shaham ,  Yehudit Natan ,  Dana Lorber ,  Peter Bartal ,  Yoram Amsalem ,  Yaniv Damari

IPC分类号： F25B19/00 ,  F25B29/00

CPC分类号： A01N1/02 ,  A01N1/0257 ,  A01N1/0263 ,  A01N1/0268

摘要： There are provided a system and a method for cryopreserving a liquid biological material disposed in a bag having a longitudinal axis. The system comprises a bag holder for holding the bag, so that the biological material therein has a surface area S, and a volume V, a tank containing a cryogenic fluid, a mechanism for the immersion of the bag holder into the tank along the longitudinal axis, an opening in the tank for insertion therethrough of the bag holder, and a guide member extending from the opening into the tank. There are further provided a system and a method for warming a cryopreserved liquid biological material disposed in a bag. The system comprises a heat source, a warming device having a space for placing the bag therein, connected to the heat source and adapted to transfer heat from the heat source to the bag, and means for maintaining the heat source in heat transfer contact with a cryogenically preserved portion of the material to allow receiving the heat by said cryogenically preserved portion.

摘要翻译： 提供了一种用于冷冻保存设置在具有纵向轴线的袋中的液体生物材料的系统和方法。 该系统包括用于保持袋的袋保持器，使得其中的生物材料具有表面积S，体积V，包含低温流体的罐，用于沿着纵向将袋保持器浸入罐中的机构 所述罐中的开口用于插入所述袋保持器，以及从所述开口延伸到所述罐中的引导构件。 还提供了一种用于加热设置在袋中的冷冻保存的液体生物材料的系统和方法。 该系统包括热源，具有用于将袋放置在其中的空间的加温装置，其连接到热源并适于将热量从热源传送到袋，以及用于将热源保持与热传递接触的装置 材料的低温保存部分以允许由所述低温保存部分接收热量。