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公开(公告)号：US20110002958A1
公开(公告)日：2011-01-06
申请号：US12791140
申请日：2010-06-01
申请人： Silvia Perri , John Polo , Yasushi Uematsu , Catherine Greer
发明人： Silvia Perri , John Polo , Yasushi Uematsu , Catherine Greer
IPC分类号： A61K39/215 , A61P31/14 , A61P31/16 , A61P31/04
CPC分类号： A61K39/12 , A61K39/145 , A61K39/155 , A61K2039/5252 , A61K2039/5256 , A61K2039/53 , A61K2039/543 , A61K2039/545 , A61K2039/55544 , A61K2039/55566 , A61K2039/70 , C12N15/86 , C12N2760/16122 , C12N2760/16134 , C12N2760/18322 , C12N2760/18334 , C12N2760/18522 , C12N2760/18534 , C12N2760/18622 , C12N2760/18634 , C12N2770/20022 , C12N2770/20034 , C12N2770/36143 , C12N2830/20 , C12N2830/60 , C12N2840/20 , C12N2840/203
摘要： Described herein are compositions and methods for stimulating an immune response to one or more proteins derived from one or more respiratory pathogens. In particular, the invention relates to alphavirus replicons, alphavirus vector constructs, and alphavirus replicon particles expressing one or more antigens derived from one or more respiratory pathogens, as well as to methods of making and using the immunogenic compositions.
摘要翻译： 本文描述了用于刺激来自一种或多种呼吸道病原体的一种或多种蛋白质的免疫应答的组合物和方法。 特别地，本发明涉及表达一种或多种源自一种或多种呼吸道病原体的抗原的甲病毒复制子，甲病毒载体构建体和表达一种或多种抗原的甲病毒属复制子颗粒，以及制备和使用免疫原性组合物的方法。
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公开(公告)号：US20060257852A1
公开(公告)日：2006-11-16
申请号：US10822303
申请日：2004-04-09
申请人： Rino Rappuoli , Vega Masignani , Konrad Stadler , Jens Gregersen , David Chien , Jang Han , John Polo , Amy Weiner , Michael Houghton , Hyun Song , Mi-Young Seo , John Donnelly , Hans Klenk , Nicholas Valiante
发明人： Rino Rappuoli , Vega Masignani , Konrad Stadler , Jens Gregersen , David Chien , Jang Han , John Polo , Amy Weiner , Michael Houghton , Hyun Song , Mi-Young Seo , John Donnelly , Hans Klenk , Nicholas Valiante
IPC分类号： C12Q1/70 , C07H21/04 , C12N15/86 , C07K14/165
CPC分类号： C07K14/005 , A61K39/00 , A61K39/12 , A61K39/215 , A61K2039/5252 , A61K2039/545 , A61K2039/55505 , A61K2039/55511 , A61K2039/55566 , C07K16/10 , C07K2317/34 , C07K2317/76 , C07K2319/21 , C12N7/00 , C12N2770/20021 , C12N2770/20022 , C12N2770/20034 , C12N2770/20043 , C12N2770/20051 , C12N2770/20063 , C12Q1/701 , G01N2333/165 , G01N2469/20
摘要： An outbreak of a virulent respiratory virus, now known as Severe Acute Respiratory Syndrome (SARS), was identified in Hong Kong, China and a growing number of countries around the world in 2003. The invention relates to nucleic acids and proteins from the SARS coronavirus. These nucleic acids and proteins can be used in the preparation and manufacture of vaccine formulations, diagnostic reagents, kits, etc. The invention also provides methods for treating SARS by administering small molecule antiviral compounds, as well as methods of identifying potent small molecules for the treatment of SARS.
摘要翻译： 在2003年，中国香港和世界各地越来越多的国家确定了现在称为严重急性呼吸综合征（SARS）的毒性呼吸道病毒的爆发。本发明涉及SARS冠状病毒的核酸和蛋白质 。 这些核酸和蛋白质可用于制备和制造疫苗制剂，诊断试剂，试剂盒等。本发明还提供了通过施用小分子抗病毒化合物治疗SARS的方法，以及鉴定用于治疗SARS的有效小分子的方法 治疗SARS。
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3.发明授权Compositions and methods for generating an immune response utilizing alphavirus-based vector systems 有权使用基于甲病毒的载体系统产生免疫应答的组合物和方法公开(公告)号：US07771979B2
公开(公告)日：2010-08-10
申请号：US11712044
申请日：2007-02-27
申请人： John Polo , Thomas W. Dubensky, Jr. , Ilya Frolov , Jason P. Gardner , Gillis Otten , Susan Barnett , David A. Driver
发明人： John Polo , Thomas W. Dubensky, Jr. , Ilya Frolov , Jason P. Gardner , Gillis Otten , Susan Barnett , David A. Driver
CPC分类号： C07K14/005 , A61K35/00 , A61K2039/5256 , C12N15/86 , C12N2740/16122 , C12N2770/36143
摘要： Methods are provided for generating immune responses utilizing alphavirus-based vector systems.
摘要翻译： 提供了使用基于甲病毒的载体系统产生免疫应答的方法。
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公开(公告)号：US20090104226A1
公开(公告)日：2009-04-23
申请号：US11597347
申请日：2005-05-20
申请人： Silvia Perri , John Polo , Yasushi Uematsu , Catherine Greer
发明人： Silvia Perri , John Polo , Yasushi Uematsu , Catherine Greer
IPC分类号： A61K39/00
CPC分类号： A61K39/12 , A61K39/145 , A61K39/155 , A61K2039/5252 , A61K2039/5256 , A61K2039/53 , A61K2039/543 , A61K2039/545 , A61K2039/55544 , A61K2039/55566 , A61K2039/70 , C12N15/86 , C12N2760/16122 , C12N2760/16134 , C12N2760/18322 , C12N2760/18334 , C12N2760/18522 , C12N2760/18534 , C12N2760/18622 , C12N2760/18634 , C12N2770/20022 , C12N2770/20034 , C12N2770/36143 , C12N2830/20 , C12N2830/60 , C12N2840/20 , C12N2840/203
摘要： Described herein are compositions and methods for stimulating an immune response to one or more proteins derived from one or more respiratory pathogens. In particular, the invention relates to alphavirus replicons, alphavirus vector constructs, alphavirus replicon particles expressing one or more antigens derived from one or more respiratory pathogens as well as to method of making and using these immunogenic compositions.
摘要翻译： 本文描述了用于刺激来自一种或多种呼吸道病原体的一种或多种蛋白质的免疫应答的组合物和方法。 特别地，本发明涉及甲病毒复制子，甲病毒载体构建体，表达一种或多种来源于一种或多种呼吸道病原体的抗原的甲病毒复制子颗粒以及制备和使用这些免疫原性组合物的方法。
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公开(公告)号：US20080069804A1
公开(公告)日：2008-03-20
申请号：US11597452
申请日：2005-05-20
申请人： Zequn Tang , Silvia Perri , John Polo
发明人： Zequn Tang , Silvia Perri , John Polo
CPC分类号： C12N15/86 , C12N2510/00 , C12N2770/36143
摘要： Provided herein are modified, functional 5′ amplification sequences that are defective packaging signals. Also provided are compositions and methods comprising these modified 5′ amplification sequences.
摘要翻译： 本文提供了作为有缺陷的包装信号的修饰的功能性5'扩增序列。 还提供了包含这些修饰的5'扩增序列的组合物和方法。
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公开(公告)号：US20080026071A1
公开(公告)日：2008-01-31
申请号：US11653792
申请日：2007-01-16
申请人： Derek O'Hagan , Gillis Otten , John Donnelly , John Polo , Susan Barnett , Manmohan Singh , Jeffrey Ulmer , Thomas Dubensky
发明人： Derek O'Hagan , Gillis Otten , John Donnelly , John Polo , Susan Barnett , Manmohan Singh , Jeffrey Ulmer , Thomas Dubensky
IPC分类号： A61K39/21 , A61K39/00 , A61K39/12 , A61K39/125 , A61K39/29 , A61P31/04 , A61P33/00 , A61P31/12 , A61K9/50 , A61K39/215 , A61K39/145 , A61K39/155
CPC分类号： C12N15/86 , A61K9/1647 , A61K9/167 , A61K39/00 , A61K39/12 , A61K39/21 , A61K47/6927 , A61K2039/53 , A61K2039/545 , A61K2039/55505 , A61K2039/55555 , A61K2039/55566 , A61K2039/57 , A61K2039/6093 , C12N2740/16134 , C12N2740/16234 , C12N2770/36143 , Y02A50/388
摘要： Microparticles with adsorbent surfaces, methods of making such microparticles, and uses thereof, are disclosed. The microparticles comprise a polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and the like, and are formed using cationic, anionic, or nonionic detergents. Also provided are microparticles in the form of submicron emulsions of an oil droplet emulsion having a metabolizable oil and an emulsifying agent. The surface of the microparticles efficiently adsorb polypeptides, such as antigens, and nucleic acids, such as ELVIS vectors and other vector constructs, containing heterologous nucleotide sequences encoding biologically active macromolecules, such as polypeptides, antigens, and adjuvants. Methods of stimulating an immune response, methods of immunizing a host animal against a viral, bacterial, or parasitic infection, and uses of the microparticle compositions for vaccines are also provided.
摘要翻译： 公开了具有吸附剂表面的微粒，制备这种微粒的方法及其用途。 微粒包含聚（α-羟基酸），多羟基丁酸，聚己内酯，聚原酸酯，聚酐等聚合物，并使用阳离子，阴离子或非离子型洗涤剂形成。 还提供了具有可代谢油和乳化剂的油滴乳液的亚微米乳液形式的微粒。 微粒的表面有效地吸收多肽，如抗原和核酸，如ELVIS载体和其他载体构建体，其含有编码生物活性大分子如多肽，抗原和佐剂的异源核苷酸序列。 还提供了刺激免疫应答的方法，免疫宿主动物免受病毒，细菌或寄生虫感染的方法，以及微粒组合物用于疫苗的用途。
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公开(公告)号：US06242259B1
公开(公告)日：2001-06-05
申请号：US09476299
申请日：1999-12-30
IPC分类号： C12N510
CPC分类号： C12N7/00 , A61K48/00 , C07K14/005 , C07K2319/00 , C07K2319/02 , C12N15/86 , C12N2770/36122 , C12N2770/36143 , C12N2770/36152 , C12N2830/00 , C12N2830/60
摘要： Nucleic acid molecule are provided comprising a nucleic acid sequence which encodes, in order, an alphavirus capsid, a signal peptide, and an alphavirus E1 or E2 glycoprotein. Also provided are vectors encoding such nucleic acid molecules, and use of such vectors or expression cassettes to generate recombinant alphavirus particles and alphavirus packaging cell lines. In addition, modified alphavirus vector constructs are provided that permit reduced transgene expression during vector packaging, as well as methods of using such vector constructs for the production of alphavirus vector particles.
摘要翻译： 提供核酸分子，其包含按顺序编码甲病毒衣壳，信号肽和甲病毒属E1或E2糖蛋白的核酸序列。 还提供了编码这种核酸分子的载体，以及使用这样的载体或表达盒产生重组甲病毒颗粒和甲病毒包装细胞系。 此外，提供了修饰的甲病毒载体构建体，其允许在载体包装期间降低的转基因表达，以及使用这种载体构建体用于产生甲病毒载体颗粒的方法。
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公开(公告)号：US09024001B2
公开(公告)日：2015-05-05
申请号：US11597452
申请日：2005-05-20
申请人： Zequn Tang , Silvia Perri , John Polo
发明人： Zequn Tang , Silvia Perri , John Polo
CPC分类号： C12N15/86 , C12N2510/00 , C12N2770/36143
摘要： Provided herein are modified, functional 5′ amplification sequences that are defective packaging signals. Also provided are compositions and methods comprising these modified 5′ amplification sequences.
摘要翻译： 本文提供了作为有缺陷的包装信号的修饰的功能性5'扩增序列。 还提供了包含这些修饰的5'扩增序列的组合物和方法。
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9.发明申请Compositions and methods for generating an immune response utilizing alphavirus-based vector systems 有权使用基于甲病毒的载体系统产生免疫应答的组合物和方法公开(公告)号：US20080226598A1
公开(公告)日：2008-09-18
申请号：US11712044
申请日：2007-02-27
申请人： John Polo , Thomas W. Dubensky , Ilya Frolov , Jason P. Gardner , Gillis Otten , Susan Barnett , David A. Driver
发明人： John Polo , Thomas W. Dubensky , Ilya Frolov , Jason P. Gardner , Gillis Otten , Susan Barnett , David A. Driver
CPC分类号： C07K14/005 , A61K35/00 , A61K2039/5256 , C12N15/86 , C12N2740/16122 , C12N2770/36143
摘要： Methods are provided for generating immune responses utilizing alphavirus-based vector systems.
摘要翻译： 提供了使用基于甲病毒的载体系统产生免疫应答的方法。
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公开(公告)号：US20050112752A1
公开(公告)日：2005-05-26
申请号：US10881575
申请日：2004-06-29
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N2770/36151 , C12Q1/6897 , C12Q1/701
摘要： Methods of production and purification for viruses and virus-derived vectors, including those related to alphaviruses, are disclosed. In one aspect, methods of purification that subject alphavirus replicon particle preparations to one or more steps of chromatographic purification, such as using an ion exchange resin, are provided. Also disclosed are methods of characterizing alphavirus replicon particles and utilizing these materials for vaccines and gene-based therapeutics.
摘要翻译： 公开了病毒和病毒衍生载体的生产和纯化方法，包括与甲病毒相关的方法。 一方面，提供将甲病毒复制子颗粒制备物进行一步或多步色谱纯化的纯化方法，例如使用离子交换树脂。 还公开了表征甲病毒复制子颗粒并利用这些材料用于疫苗和基于基因的治疗剂的方法。
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