A frequency multiplier and a delay-reused duty cycle calibration method thereof are provided. The frequency multiplier includes a first calibration circuit, a second calibration circuit and a controller. In a calibration mode of the frequency multiplier, an output terminal of a delay cell is coupled to an input terminal of the delay cell. The first calibration circuit repeatedly uses the delay cell M times for generating a first delayed signal. The controller controls the delay cell according to the first delayed signal, to find a delay of the delay cell which makes M times the delay be equal to one cycle period of an input clock signal. After the delay is found, the delay cell is repeatedly used M/2 times for generating a second delayed signal. The controller controls the second calibration circuit according to the second delayed signal to make an input calibration signal have a target duty cycle.