- 专利标题： Ketoreductase polypeptides and polynucleotides
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申请号： US16605576申请日： 2018-04-13
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公开(公告)号： US11021729B2公开(公告)日： 2021-06-01
- 发明人: Jeffrey C. Moore , Jack Liang , Jonathan Penfield , Jovana Nazor , Nikki Dellas , Vesna Mitchell , Da Duan , Iman Farasat , Agustina Rodriguez-Granillo , Grant Murphy , Nicholas Marshall
- 申请人： Codexis, Inc.
- 申请人地址： US CA Redwood City
- 专利权人： Codexis, Inc.
- 当前专利权人： Codexis, Inc.
- 当前专利权人地址： US CA Redwood City
- 代理机构： Codexis, Inc.
- 国际申请： PCT/US2018/027450 WO 20180413
- 国际公布： WO2018/200214 WO 20181101
- 主分类号： C12P41/00
- IPC分类号： C12P41/00 ; C12N9/02 ; C12N9/04 ; C12N15/70
摘要：
The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.
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