The invention discloses a flexible operation clamping mechanism and an operation tool. A fixing lock ring is arranged at the upper end of an elastic installing sleeve and can be fixed to a connectingplate of the mechanism, and the elastic installing sleeve is hollow, so that wire arrangement is facilitated. A fastening clamp sheet and a locking buckle are used at the lower end of the elastic installing sleeve so that an electric main shaft can be clamped and fixed, and the relative positions of the elastic installing sleeve and the electric main shaft is kept unchangeable. The elastic installing sleeve is provided with an elastic groove so that elasticity can be improved, and when the operation tool works, a certain-degree obstacle avoiding function can be generated through the electric main shaft, and the electric main shaft and the clamping mechanism are protected. The proper screw pitch and groove depth can be calculated and set according to an operation object and operation work condition requirements, and the flexible operation clamping mechanism can generate different elastic forces according to different screw pitches and groove depths. According to the characteristic thatthe flexible operation clamping mechanism can generate different elastic forces through calculation and setting of different screw pitches and groove depths, it can be known that the flexible operation clamping mechanism is low in cost, convenient to install and use and reliable in clamping. Different operation tools can be arranged below the electric main shaft so that different functions can beachieved.