The invention discloses a clarification method of ultrafiltration enzyme in the preparation of ginseng juice. The clarification method comprises the following steps: cutting clean ginseng into slices with the thickness of 5mm, adding purified water to a material-liquid ratio of 1:8-1:12, mincing by using a pulverizer, and then finely grinding ginseng pulp by using a colloid mill; adjusting pH value by adopting citric acid and lactic acid; carrying out high-temperature and high-pressure treatment; cooling, placing the ginseng pulp into an enzymolysis tank, adjusting pH value by using sodium bicarbonate, preserving heat and carrying out hydrolysis; and carrying out enzyme deactivation treatment at 100 DEG C, carrying out centrifugal treatment and filtering the ginseng pulp to obtain clarified ginseng juice. After the ginseng pulp prepared by the clarification method is treated by the ultrafiltration enzyme, macromolecular substances such as cellulose, pectin and protein are partially hydrolyzed to be in a micromolecular state, so that the ginseng juice is obviously clarified, the yield of the ginseng juice is increased, and the flavor of the ginseng juice is improved; meanwhile, the utilization rate of the ginseng can be effectively increased, the production process is easy, the operation is convenient, the cost is low, and the clarification method is suitable for large-scale industrial production.