The present invention discloses process of extracting and separating compounds I-VIII from ginseng-ophiopogon injection. These compounds have functions of inhibiting myocardial cell apoptosis, promoting the release of CO from vascular endothelial cell, suppressing oxidation caused by AAPH, etc; and this show that these compounds are main active components for the ginseng-ophiopogon injection to treat cardiac and cerebral vascular diseases. The extracting and separating process includes the steps of: chromatographic separation in macroporous resin column, MCI columm, silica gel column, glucosan gel column and reverse silica gel column; efficient liquid phase separation; sublimation; crystallization; etc. The compound structure identification is completed through infrared method, 1D and 2D nuclear magnetic resonance, mass spectrum and other spectroscopic method.