公开(公告)号：US08003771B2

公开(公告)日：2011-08-23

申请号：US12714983

申请日：2010-03-01

Applicant: Zbigniew Skrzypczynski ,  Sarah R Wayland

Inventor： Zbigniew Skrzypczynski ,  Sarah R Wayland

IPC: C07H21/00 ,  C07H21/04 ,  C07C247/00 ,  A61K31/41 ,  C12P13/06 ,  C12P13/02

CPC classification number: C07H21/00 ,  A61K49/0039 ,  A61K49/0054

Abstract: The present invention relates to compositions useful as probes and in other applications and methods of their use. In some embodiments, nucleotides are prepared and functionalized with dyes. In some embodiments a first molecule is functionalized with an alkynyl group, a second molecule is functionalized with an azide group, and said first and second molecules are mixed under conditions to form a conjugate with a 1,2,3-triazol group. In further embodiments, a nucleotide is functionalized with an alkynyl group, a dye is functionalized with an azide group, and mixing the nucleotide and the dye forms a conjugate capable of emitting light.

Abstract translation: 本发明涉及可用作探针的组合物及其应用和使用方法。 在一些实施方案中，制备核苷酸并用染料官能化。 在一些实施方案中，第一分子用炔基官能化，第二分子用叠氮基官能化，并且所述第一和第二分子在条件下混合以形成具有1,2,3-三唑基团的缀合物。 在另外的实施方案中，核苷酸用炔基官能化，染料用叠氮化物官能化，并且混合核苷酸和染料形成能发光的共轭体。