公开(公告)号：US20120265351A1

公开(公告)日：2012-10-18

申请号：US13211582

申请日：2011-08-17

申请人： XIANG YU ,  XIAO-YONG MA ,  QI CHEN

发明人： XIANG YU ,  XIAO-YONG MA ,  QI CHEN

IPC分类号： G05D9/12

CPC分类号： G05D9/12

摘要： A switch control system for controlling an electrically conductive fluid supply to a tank, includes a detection circuit, a gate circuit, a control circuit and a switch circuit. The detection circuit detects a fluid level in the tank and outputs a voltage level signal. The gate circuit receives the voltage level signal and outputs control signals accordingly. The control circuit receives the control signals and outputs a first voltage and a second voltage accordingly. The switch circuit receives the first voltage and the second voltage and turns on and turns off the electrically conductive fluid supply to the tank in accordance with the first voltage and the second voltage.

摘要翻译： 用于控制向罐的导电流体供应的开关控制系统包括检测电路，门电路，控制电路和开关电路。 检测电路检测储罐中的液位并输出电压电平信号。 门电路接收电压电平信号，并相应地输出控制信号。 控制电路接收控制信号并相应地输出第一电压和第二电压。 开关电路接收第一电压和第二电压，并根据第一电压和第二电压接通和断开对槽的导电流体供应。

公开(公告)号：US20100227361A1

公开(公告)日：2010-09-09

申请号：US12575550

申请日：2009-10-08

申请人： QI CHEN ,  Qiong Cheng ,  Kevin Michael Croker ,  Thomas Eduard Friedmann ,  Kristin Ruebling-Jass

发明人： QI CHEN ,  Qiong Cheng ,  Kevin Michael Croker ,  Thomas Eduard Friedmann ,  Kristin Ruebling-Jass

IPC分类号： C12P21/00 ,  C12N1/21

CPC分类号： C07K14/245 ,  C07K2319/00 ,  C07K2319/50 ,  C12N15/70

摘要： Disrupting the expression of endogenous Escherichia host cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production. The addition of a genetic modification to the coding region of gene yejM further enhances peptide production and facilitates easier downstream processing. Recombinant Escherichia host cells are provided as well as methods of using such host cells for heterologous peptide production.

摘要翻译： 破坏内源性大肠杆菌宿主细胞基因gcvA和spr的表达提供具有增加的异源肽产生的突变宿主细胞。 在基因yejM的编码区添加遗传修饰进一步增强了肽的产生，并且便于下游处理。 提供重组埃希氏杆菌宿主细胞以及使用这种宿主细胞进行异源肽生产的方法。

公开(公告)号：US20120122168A1

公开(公告)日：2012-05-17

申请号：US12946120

申请日：2010-11-15

申请人： QI CHEN ,  QIONG CHENG ,  ANDREW C. ELIOT ,  Kristin Ruebling-Jass

发明人： QI CHEN ,  QIONG CHENG ,  ANDREW C. ELIOT ,  Kristin Ruebling-Jass

IPC分类号： C12N1/21 ,  C12P7/18 ,  C12P7/42 ,  C12P7/20

CPC分类号： C12P7/20 ,  C12N9/93 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12Y602/01001

摘要： Recombinant E. coli having enhanced acetyl-CoA synthetase activity and the ability to produce glycerol and glycerol-derived products, such as 3-hydroxypropionic acid, methylglyoxal, 1,2-propanediol, and 1,3-propanediol, are described. The recombinant E. coli comprise a promoter operably linked to a nucleotide sequence encoding a polypeptide having acetyl-CoA synthetase enzyme activity, wherein the promoter and nucleotide sequence are each independently either native or non-native.

摘要翻译： 描述了具有增强的乙酰辅酶A合成酶活性和产生甘油和甘油衍生产物如3-羟基丙酸，甲基乙二醛，1,2-丙二醇和1,3-丙二醇的能力的重组大肠杆菌。 重组大肠杆菌包含与编码具有乙酰-CoA合成酶活性的多肽的核苷酸序列可操作地连接的启动子，其中启动子和核苷酸序列各自独立地是天然的或非天然的。