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1.发明授权Host cell modifications that improve peptide production and downstream processing 失效改善肽生产和下游加工的宿主细胞修饰公开(公告)号：US07915011B2
公开(公告)日：2011-03-29
申请号：US12575550
申请日：2009-10-08
CPC分类号： C07K14/245 , C07K2319/00 , C07K2319/50 , C12N15/70
摘要： Disrupting the expression of endogenous Escherichia host cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production. The addition of a genetic modification to the coding region of gene yejM further enhances peptide production and facilitates easier downstream processing. Recombinant Escherichia host cells are provided as well as methods of using such host cells for heterologous peptide production.
摘要翻译： 破坏内源性大肠杆菌宿主细胞基因gcvA和spr的表达提供具有增加的异源肽产生的突变宿主细胞。 在基因yejM的编码区添加遗传修饰进一步增强了肽的产生，并且便于下游处理。 提供重组埃希氏杆菌宿主细胞以及使用这种宿主细胞进行异源肽生产的方法。
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2.发明申请公开(公告)号：US20100227361A1
公开(公告)日：2010-09-09
申请号：US12575550
申请日：2009-10-08
CPC分类号： C07K14/245 , C07K2319/00 , C07K2319/50 , C12N15/70
摘要： Disrupting the expression of endogenous Escherichia host cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production. The addition of a genetic modification to the coding region of gene yejM further enhances peptide production and facilitates easier downstream processing. Recombinant Escherichia host cells are provided as well as methods of using such host cells for heterologous peptide production.
摘要翻译： 破坏内源性大肠杆菌宿主细胞基因gcvA和spr的表达提供具有增加的异源肽产生的突变宿主细胞。 在基因yejM的编码区添加遗传修饰进一步增强了肽的产生，并且便于下游处理。 提供重组埃希氏杆菌宿主细胞以及使用这种宿主细胞进行异源肽生产的方法。
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3.发明授权公开(公告)号：US07572643B2
公开(公告)日：2009-08-11
申请号：US11284155
申请日：2005-11-21
申请人： Kevin Michael Croker , Michael B. Damore , Kostantinos Kourtakis , Michael P. Perry , James M. Prober , Paul Douglas Stull
发明人： Kevin Michael Croker , Michael B. Damore , Kostantinos Kourtakis , Michael P. Perry , James M. Prober , Paul Douglas Stull
IPC分类号： G01N33/551 , G01N33/552 , G01N33/553
CPC分类号： G01N33/54346 , B82Y30/00
摘要： A microsphere for use in a bioassay comprising a glass core coated with a nanoparticle composite comprising a bioactive probe is provided. The nanoparticle composite coating enhances the density of bioprobe loading on the surface of the microspheres, resulting in enhanced dynamic range and sensitivity in bioassays. The particle may be used in detection systems where resonant light scattering properties of the particle are useful.
摘要翻译： 提供了用于生物测定的微球，其包括涂覆有包含生物活性探针的纳米颗粒复合物的玻璃核心。 纳米颗粒复合涂层增强了微球表面生物探针加载的密度，从而提高了生物测定中的动态范围和灵敏度。 该颗粒可用于其中共聚光散射性质有用的检测系统中。
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公开(公告)号：US20100159513A1
公开(公告)日：2010-06-24
申请号：US12619790
申请日：2009-11-17
CPC分类号： C12P21/02
摘要： Several endogenous genes have been identified in Escherichia coli, the overexpression of which increases recombinant peptide production. Increasing the copy number of aroB, aroK, proB, or crl increases the amount of a recombinant peptide produced by a host cell. Recombinant host cells comprising at least one chimeric genetic construct encoding a peptide of interest and at least one genetic modification that increases recombinant peptide production are provided as well as methods of using such recombinant host cells
摘要翻译： 已经在大肠杆菌中鉴定了几种内源基因，其过表达增加了重组肽的产生。 增加aroB，aroK，proB或crl的拷贝数增加了由宿主细胞产生的重组肽的量。 提供了包含至少一种编码目的肽的嵌合基因构建体和至少一种提高重组肽产生的遗传修饰的重组宿主细胞以及使用这种重组宿主细胞的方法
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公开(公告)号：US07662587B1
公开(公告)日：2010-02-16
申请号：US12398358
申请日：2009-03-05
CPC分类号： C12N15/70 , C07K14/245 , C07K2319/00 , C07K2319/50
摘要： Disrupting the expression of endogenous Escherichia host cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production relative to control Escherichia host cells. Recombinant Escherichia host cells are provided as well as methods of using such host cells for heterologous peptide production.
摘要翻译： 破坏内源性大肠杆菌宿主细胞基因gcvA和spr的表达提供相对于对照大肠杆菌宿主细胞具有增加的异源肽产生的突变宿主细胞。 提供重组埃希氏杆菌宿主细胞以及使用这种宿主细胞进行异源肽生产的方法。
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6.发明申请USE OF TETRACYSTEINE TAGS IN FLUORESCENCE-ACTIVATED CELL SORTING ANALYSIS OF PROKARYOTIC CELLS PRODUCING PEPTIDES OR PROTEINS 失效在生产肽或蛋白质的原核细胞的荧光激活细胞分选中使用四肽蛋白标签公开(公告)号：US20090117609A1
公开(公告)日：2009-05-07
申请号：US12263608
申请日：2008-11-03
申请人： Qiong Cheng , Kevin Michael Croker , Stephen R. Fahnestock , Tanja Maria Gruber , Kristin Ruebling-Jass , Jianzhong Zhang
发明人： Qiong Cheng , Kevin Michael Croker , Stephen R. Fahnestock , Tanja Maria Gruber , Kristin Ruebling-Jass , Jianzhong Zhang
CPC分类号： C12N15/62 , C07K2319/20 , C07K2319/60
摘要： A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected.
摘要翻译： 体内标记和鉴定重组产生的肽或蛋白质在未经过预处理的原核宿主细胞内的过程。 表达含有至少一个四羧酸标签的融合肽的重组原核细胞在体内用双标记标记试剂进行标记。 使用荧光活化细胞分选器来鉴定和选择荧光细胞亚群，其中细胞中融合肽的量与所检测的荧光量成比例。
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7.发明授权Use of tetracysteine tags in fluorescence-activated cell sorting analysis of prokaryotic cells producing peptides or proteins 失效在用于产生肽或蛋白质的原核细胞的荧光激活细胞分选分析中使用四羧酸标签公开(公告)号：US07794963B2
公开(公告)日：2010-09-14
申请号：US12263608
申请日：2008-11-03
申请人： Qiong Cheng , Kevin Michael Croker , Stephen R. Fahnestock , Tanja Maria Gruber , Kristin Ruebling-Jass , Jianzhong Zhang
发明人： Qiong Cheng , Kevin Michael Croker , Stephen R. Fahnestock , Tanja Maria Gruber , Kristin Ruebling-Jass , Jianzhong Zhang
IPC分类号： G01N33/569 , C07K16/00
CPC分类号： C12N15/62 , C07K2319/20 , C07K2319/60
摘要： A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected.
摘要翻译： 体内标记和鉴定重组产生的肽或蛋白质在未经过预处理的原核宿主细胞内的过程。 表达含有至少一个四羧酸标签的融合肽的重组原核细胞在体内用双标记标记试剂进行标记。 使用荧光活化细胞分选器来鉴定和选择荧光细胞亚群，其中细胞中融合肽的量与所检测的荧光量成比例。
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