公开(公告)号：US11981953B2

公开(公告)日：2024-05-14

申请号：US16369939

申请日：2019-03-29

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： Christina Marie Hull ,  Mingwei Huang ,  Sebastien Claude Ortiz

IPC: C12Q1/18 ,  A01N33/10 ,  A01N37/44 ,  A01N41/12 ,  A01N47/40 ,  A61K31/137 ,  A61K31/145 ,  A61K31/155 ,  A61K31/24 ,  A61P31/10 ,  C12Q1/66

CPC classification number: C12Q1/18 ,  A01N33/10 ,  A01N37/44 ,  A01N41/12 ,  A01N47/40 ,  A61K31/137 ,  A61K31/145 ,  A61K31/155 ,  A61K31/24 ,  A61P31/10 ,  C12Q1/66 ,  G01N2333/375

Abstract: A method of testing compounds for activity to inhibit germination of spores. The method includes the steps of providing bacterial, fungal, or plant spores transformed to contain and express a detectable marker, wherein the marker when expressed, is operationally linked to a spore-specific or yeast-specific protein, in a medium and under environmental conditions in which the spores will germinate, and measuring a first signal output generated by the marker prior to the spores initiating germination; contacting the spores of step (a) with a compound whose activity to inhibit germination of spores is to be measured; incubating the spores of step (b) under environmental conditions and for a time wherein spores not treated with the compound will germinate; and determining extent of germination of the spores by measuring a second signal output generated by the marker, wherein a difference between the first signal output and the second signal output is proportional to the extent of germination of the spores. Also described are compositions of matter for inhibiting spore germination in vitro and in vivo.

公开(公告)号：US20240247299A1

公开(公告)日：2024-07-25

申请号：US18631396

申请日：2024-04-10

Applicant: Wisconsin Alumni Research Foundation

Inventor： Christina Marie HULL ,  Mingwei HUANG ,  Sebastien Claude Ortiz

IPC: C12Q1/18 ,  A01N33/10 ,  A01N37/44 ,  A01N41/12 ,  A01N47/40 ,  A61K31/137 ,  A61K31/145 ,  A61K31/155 ,  A61K31/24 ,  A61P31/10 ,  C12Q1/66

CPC classification number: C12Q1/18 ,  A01N33/10 ,  A01N37/44 ,  A01N41/12 ,  A01N47/40 ,  A61K31/137 ,  A61K31/145 ,  A61K31/155 ,  A61K31/24 ,  A61P31/10 ,  C12Q1/66 ,  G01N2333/375

Abstract: A method of testing compounds for activity to inhibit germination of spores. The method includes the steps of (a) providing bacterial, fungal, or plant spores transformed to contain and express a detectable marker, wherein the marker when expressed, is operationally linked to a spore-specific or yeast-specific protein, in a medium and under environmental conditions in which the spores will germinate, and measuring a first signal output generated by the marker prior to the spores initiating germination; (b) contacting the spores of step (a) with a compound whose activity to inhibit germination of spores is to be measured; (c) incubating the spores of step (b) under environmental conditions and for a time wherein spores not treated with the compound will germinate; and (d) determining extent of germination of the spores by measuring a second signal output generated by the marker, wherein a difference between the first signal output and the second signal output is proportional to the extent of germination of the spores. Also described are compositions of matter for inhibiting spore germination in vitro and in vivo.

公开(公告)号：US20190300926A1

公开(公告)日：2019-10-03

申请号：US16369939

申请日：2019-03-29

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： Christina Marie Hull ,  Mingwei Huang ,  Sebastien Claude Ortiz

IPC: C12Q1/18 ,  C12Q1/66 ,  A01N41/12 ,  A01N47/40 ,  A01N37/44 ,  A01N33/10 ,  A61K31/145 ,  A61K31/155 ,  A61K31/24 ,  A61K31/137 ,  A61P31/10

Abstract: A method of testing compounds for activity to inhibit germination of spores. The method includes the steps of providing bacterial, fungal, or plant spores transformed to contain and express a detectable marker, wherein the marker when expressed, is operationally linked to a spore-specific or yeast-specific protein, in a medium and under environmental conditions in which the spores will germinate, and measuring a first signal output generated by the marker prior to the spores initiating germination; contacting the spores of step (a) with a compound whose activity to inhibit germination of spores is to be measured; incubating the spores of step (b) under environmental conditions and for a time wherein spores not treated with the compound will germinate; and determining extent of germination of the spores by measuring a second signal output generated by the marker, wherein a difference between the first signal output and the second signal output is proportional to the extent of germination of the spores. Also described are compositions of matter for inhibiting spore germination in vitro and in vivo.