公开(公告)号：US20070009937A1

公开(公告)日：2007-01-11

申请号：US11437163

申请日：2006-05-19

申请人： Ulrich Laemmli ,  Manfred Schmid

发明人： Ulrich Laemmli ,  Manfred Schmid

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  C12Q2522/10 ,  C12Q2521/301

摘要： The present invention is directed to a method for localising, in chromatin or DNA, the binding loci of a chromatin or DNA binding protein comprising the following steps: a) tethering, directly or indirectly, an enzyme with regulatable activity to said chromatin binding protein, b) transiently activating the regulatable enzymatic activity bound to the targeted chromatin and c) mapping the enzymatically-modified genomic sites introduced by the enzyme into the chromatin. According to an embodiment of the invention, the regulatable enzyme is a regulatable protease. The mapping step may be carried out on specific DNA fragments, or on a chromosome-wide scale or a genome-wide scale. The present invention is also directed to kits for carrying out the methods of the invention.

摘要翻译： 本发明涉及一种用于在染色质或DNA中定位染色质或DNA结合蛋白的结合位点的方法，包括以下步骤：a）直接或间接地将具有可调节活性的酶系到所述染色质结合蛋白上， b）瞬时激活与靶向染色质结合的可调节酶活性，和c）将由酶引入的酶修饰的基因组位点映射到染色质中。 根据本发明的一个实施方案，可调节酶是可调节蛋白酶。 映射步骤可以在特定的DNA片段上进行，或者在染色体大范围或全基因组范围内进行。 本发明还涉及用于实施本发明方法的试剂盒。