公开(公告)号：US20230003711A1

公开(公告)日：2023-01-05

申请号：US17930865

申请日：2022-09-09

申请人： UNM RAINFOREST INNOVATIONS

发明人： Steven R.J. BRUECK ,  Jeremy Scott EDWARDS ,  Alexander NEUMANN ,  Yuliya KUZNETSOVA ,  Edgar A. MENDOZA

IPC分类号： G01N33/487 ,  C12Q1/6869 ,  B01L3/00 ,  C23C16/34 ,  C23C16/455 ,  C23C16/50 ,  G01N21/65

摘要： Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

公开(公告)号：US20240118260A1

公开(公告)日：2024-04-11

申请号：US18528948

申请日：2023-12-05

申请人： UNM RAINFOREST INNOVATIONS

发明人： Steven R.J. BRUECK ,  Jeremy Scott EDWARDS ,  Alexander NEUMANN ,  Yuliya KUZNETSOVA ,  Edgar A. MENDOZA

IPC分类号： G01N33/487 ,  B01L3/00 ,  C12Q1/6869 ,  C23C16/34 ,  C23C16/455 ,  C23C16/50 ,  G01N21/65

CPC分类号： G01N33/48721 ,  B01L3/502707 ,  B01L3/502761 ,  C12Q1/6869 ,  C23C16/345 ,  C23C16/45525 ,  C23C16/50 ,  G01N21/65 ,  B01L2200/0663 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0896 ,  B01L2300/16 ,  B01L2300/168 ,  B01L2400/0421 ,  G01N2021/653 ,  G01N2201/061

摘要： Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.