公开(公告)号：US20230309552A1

公开(公告)日：2023-10-05

申请号：US18043108

申请日：2022-05-25

申请人： UNIVERSITY OF SHANGHAI FOR SCIENCE AND TECHNOLOGY

发明人： Weijie LI ,  Jing SHEN ,  Yanbin QIN ,  Zhixin LI ,  Hongli YAN ,  Qin QI ,  Baolin LIU

IPC分类号： A01N1/02

CPC分类号： A01N1/0284 ,  A01N1/0252

摘要： A method, system and device for programmed cell freezing is disclosed. The method includes: S1, acquiring a cooling rate k and a temperature distribution function T=a×h2+b×h+c of a heat preservation chamber containing liquid nitrogen; S2, controlling a cell preservation tube to lower in the chamber at a speed of v=(k-b)/2ah, and acquiring temperatures T1 and T2 inside and outside the tube at a same time and at a same height in real time during the lowering of the tube; S3, determining whether a difference Δt between T1 and T2 exceeds a preset temperature difference threshold, if yes, proceeding to S4, and if no, proceeding to S2 until the tube reaches a position corresponding to a preset temperature T0; and S4, controlling the cell preservation tube to stop until the difference Δt between T1 and T2 is less than or equal to the temperature difference threshold, and continuing S2.