公开(公告)号：US10942346B2

公开(公告)日：2021-03-09

申请号：US15544890

申请日：2016-02-02

申请人： The Hong Kong University of Science and Technology

发明人： Ming Tak Michael Loy ,  Shengwang Du ,  Teng Zhao ,  Sze Cheung Lau ,  Ying Wang ,  Yumian Su

IPC分类号： G02B21/36 ,  G02B21/08 ,  G02B27/09 ,  G02B21/16 ,  G02B27/58 ,  G02B21/00

摘要： Methods and systems for generating non-diffracting light sheets for multicolor fluorescence microscopy are disclosed. A method for generating a non-diffracting light patterned Bessel sheet comprises transmitting an input light beam through a Fourier transform lens the input light beam has a spatial intensity pattern at a first plane, and a Fourier plane is formed after the Fourier transform lens to obtain a first light beam; transmitting the first light beam through an annulus mask to obtain a second light beam; and transmitting the second light beam through an excitation objective lens to form a non-diffracting patterned light sheet. A method for generating a non-diffracting light line Bessel sheet comprises transmitting an input light beam at a first lane through an annulus mask to obtain a first light beam; and transmitting the first light beam through an excitation objective lens to form a non-diffracting Bessel light sheet.