公开(公告)号：US20230349011A1

公开(公告)日：2023-11-02

申请号：US18137350

申请日：2023-04-20

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Iwijn de Vlaminck ,  Michael Kertesz ,  Kiran Khush ,  Mark Kowarsky ,  Lance Martin ,  Stephen R. Quake ,  Hannah Valantine

IPC: C12Q1/70 ,  G16B30/00 ,  C12Q1/6883 ,  G16B20/00 ,  G16B30/10 ,  G16B20/20 ,  C12Q1/689 ,  C12Q1/6895

CPC classification number: C12Q1/705 ,  G16B30/00 ,  C12Q1/6883 ,  G16B20/00 ,  G16B30/10 ,  G16B20/20 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q1/701

Abstract: Methods, devices, compositions and kits are provided for analysis of the microbiome or individual components thereof in an individual. The methods find use in a determination of infection, in analysis of the microbiome structure, in determining the immunocompetence of an individual, and the like. In some embodiments of the invention, the individual is treated with an therapeutic regimen, e.g. drugs, diet, radiation therapy, and the like.

公开(公告)号：US11597966B2

公开(公告)日：2023-03-07

申请号：US17836671

申请日：2022-06-09

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Stephen R. Quake ,  Thomas M. Snyder ,  Hannah Valantine

IPC: C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6837 ,  G16B20/00 ,  G16B20/20 ,  C12Q1/6883 ,  C12Q1/6858

Abstract: The invention provides methods, devices, compositions and kits for diagnosing or predicting transplant status or outcome in a subject who has received a transplant.

公开(公告)号：US20220372576A1

公开(公告)日：2022-11-24

申请号：US17836671

申请日：2022-06-09

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Stephen R. Quake ,  Thomas M. Snyder ,  Hannah Valantine

IPC: C12Q1/6883

Abstract: The invention provides methods, devices, compositions and kits for diagnosing or predicting transplant status or outcome in a subject who has received a transplant.

公开(公告)号：US20220125310A1

公开(公告)日：2022-04-28

申请号：US17568087

申请日：2022-01-04

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Yosef Mandel ,  Ismail Emre Araci ,  Stephen R. Quake

IPC: A61B3/16 ,  A61B90/00 ,  A61B5/00

Abstract: Glaucoma is the second most common cause of blindness in the global world. It is a multifactorial disease with several risk factors, of which intraocular pressure (IOP) is the most important. IOP measurements are used for glaucoma diagnosis and patient monitoring. IOP has wide diurnal fluctuation, and is dependent on body posture, so the occasional measurements done by the eye care expert in clinic can be misleading. We provide an implantable sensor, based on microfluidic principles, which in one example has 1 mmHg limit of detection, high sensitivity and excellent reproducibility. This sensor has an optical interface, which enables IOP to be read with, for example, a cell phone camera. The design, fabrication, along with the option of self-monitoring are promising steps toward better patient care and treatment for this devastating disease.

公开(公告)号：US11213203B2

公开(公告)日：2022-01-04

申请号：US16031044

申请日：2018-07-10

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Yosef Mandel ,  Ismail Emre Araci ,  Stephen R. Quake

IPC: A61B90/00 ,  A61B3/16 ,  A61B5/00

Abstract: Glaucoma is the second most common cause of blindness in the global world. It is a multifactorial disease with several risk factors, of which intraocular pressure (IOP) is the most important. IOP measurements are used for glaucoma diagnosis and patient monitoring. IOP has wide diurnal fluctuation, and is dependent on body posture, so the occasional measurements done by the eye care expert in clinic can be misleading. We provide an implantable sensor, based on microfluidic principles, which in one example has 1 mmHg limit of detection, high sensitivity and excellent reproducibility. This sensor has an optical interface, which enables IOP to be read with, for example, a cell phone camera. The design, fabrication, along with the option of self-monitoring are promising steps toward better patient care and treatment for this devastating disease.

公开(公告)号：US20210363584A1

公开(公告)日：2021-11-25

申请号：US17396457

申请日：2021-08-06

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Lian Chye Winston Koh ,  Stephen R. Quake ,  Hei-Mun Christina Fan ,  Wenying Pan

IPC: C12Q1/6883 ,  G16B50/00 ,  C12Q1/6809 ,  C12Q1/6874 ,  C12Q1/6876 ,  G16H10/40

Abstract: The invention generally relates to methods for assessing the health of a tissue by characterizing circulating nucleic acids in a biological sample. According to certain embodiments, methods for assessing the health of a tissue include the steps of detecting a sample level of RNA in a biological sample, comparing the sample level of RNA to a reference level of RNA specific to the tissue, determining whether a difference exists between the sample level and the reference level, and characterizing the tissue as abnormal if a difference is detected.

公开(公告)号：US20210317198A1

公开(公告)日：2021-10-14

申请号：US17351053

申请日：2021-06-17

Applicant: Chan Zuckerberg Biohub, Inc. ,  The Board of Trustees of the Leland Stanford Junior University

Inventor： Derek Croote ,  Stephen R. Quake ,  Kari Nadeau ,  Spyros Darmanis ,  David N. Cornfield

IPC: C07K16/18 ,  C07K16/14 ,  C07K16/16

Abstract: In one aspect, methods of generating human monoclonal antibodies that specifically binds to an allergen are provided. In some embodiments, the monoclonal antibodies are generated from sequences identified from isolated single B cells from a human subject who is allergic to the allergen.

公开(公告)号：US20210246195A1

公开(公告)日：2021-08-12

申请号：US17056384

申请日：2019-05-17

Applicant: Chan Zuckerberg Biohub, Inc. ,  The Board of Trustees of the Leland Stanford Junior University

Inventor： Derek Croote ,  Stephen R. Quake ,  Kari Nadeau ,  Spyros Darmanis ,  David N. Cornfield

IPC: C07K16/18 ,  C07K16/16 ,  C07K16/14

Abstract: In one aspect, methods of generating human monoclonal antibodies that specifically binds to an allergen are provided. In some embodiments, the monoclonal antibodies are generated from sequences identified from isolated single B cells from a human subject who is allergic to the allergen.

公开(公告)号：US10774383B2

公开(公告)日：2020-09-15

申请号：US16014900

申请日：2018-06-21

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Stephen R. Quake ,  Joshua Weinstein ,  Ning Jiang ,  Daniel S. Fisher

IPC: C12Q1/68 ,  C12Q1/6883

Abstract: High-throughput long read sequencing is used to perform immunogenomic characterization of expressed antibody repertoires in the context of vaccination. Informatic analysis allows global characterizations of isotype distributions, determination of the lineage structure of the repertoire and measure age and antigen related mutational activity. Global analysis of the immune system's clonal structure provides direct insight into the effects of vaccination and provides a detailed molecular portrait of age-related effects.

公开(公告)号：US20200277667A1

公开(公告)日：2020-09-03

申请号：US16849067

申请日：2020-04-15

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Chunxiao Song ,  Stephen R. Quake

IPC: C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6886

Abstract: Provided herein is a method of sequencing hydroxymethyated cell-free DNA. In some embodiments, the method comprises adding an affinity tag to only hydroxymethyated DNA molecules in a sample of cfDNA, enriching for the DNA molecules that are tagged with the affinity tag; and sequencing the enriched DNA molecules.