公开(公告)号：US5827714A

公开(公告)日：1998-10-27

申请号：US739015

申请日：1996-10-28

申请人： Takeshi Yamamoto ,  Motoko Nakashizuka ,  Ichiro Terada ,  Hisashi Kodama

发明人： Takeshi Yamamoto ,  Motoko Nakashizuka ,  Ichiro Terada ,  Hisashi Kodama

IPC分类号： C12N9/10 ,  C12N1/20

CPC分类号： C12N9/1081 ,  Y10S435/822

摘要： A .beta.-galactoside-.alpha.-2,6-sialyltransferase derived from a microorganism belonging to the genus Photobacterium has ben disclosed, having the following physicochemical properties: (1) action and specificity: transferring sialic acid from cytidine monophosphate-sialic acid to the 6-position of a galactose residue in a sugar chain of a glycoconjugate or in a free sugar chain, or to the 6-position of a monosaccharide having a hydroxyl group on carbon at the 6-position and being capable of forming a glycoconjugate. (2) optimum pH: 5 to 6; (3) optimum temperature: 30.degree. C.; and (4) molecular weight: 64,000.+-.5,000 (determined by gel filtration).

摘要翻译： 衍生自属于光杆菌属的微生物的β-半乳糖苷-α-2,6-唾液酸转移酶已公开，具有以下物理化学性质：（1）作用和特异性：将唾液酸从胞苷一磷酸唾液酸转移至6 在糖缀合物或游离糖链的糖链中的半乳糖残基的位置，或6-位上具有羟基的单糖的6-位，并且能够形成糖缀合物。 （2）最适pH：5〜6; （3）最适温度：30℃。 和（4）分子量：64,000 +/- 5,000（通过凝胶过滤测定）。

公开(公告)号：US5908766A

公开(公告)日：1999-06-01

申请号：US741663

申请日：1996-10-31

申请人： Takeshi Yamamoto ,  Motoko Nakashizuka ,  Ichiro Terada ,  Hisashi Kodama

发明人： Takeshi Yamamoto ,  Motoko Nakashizuka ,  Ichiro Terada ,  Hisashi Kodama

IPC分类号： C12N9/10 ,  C12P19/26 ,  C12R1/01 ,  C12P19/18 ,  C12P19/04 ,  C12P19/44

CPC分类号： C12N9/1081 ,  C12P19/26

摘要： Described is a method of production of saccharides containing sialic acid, wherein .beta.-galactoside-.alpha.-2,6-sialyltransferase is used for linking sialic acid to the 6-position of a galactose residue in a sugar chain of a glycoconjugate or the 6-position of a galactose residue in a free sugar chain, or to the 6-position of a monosaccharide having a hydroxyl group on carbon at the 6-position and being capable of forming an oligosaccharide or a glycoconjugate.

摘要翻译： 描述了一种生产含有唾液酸的糖的方法，其中使用β-半乳糖苷-α-2,6-唾液酸转移酶将唾液酸与糖缀合物的糖链中的半乳糖残基的6-位连接， 在游离糖链中的半乳糖残基的位置，或6-位上具有羟基的单糖的6-位，并且能够形成寡糖或糖缀合物。

公开(公告)号：US06255094B1

公开(公告)日：2001-07-03

申请号：US09171878

申请日：1999-01-05

申请人： Takeshi Yamamoto ,  Motoko Nakashizuka ,  Ichiro Terada

发明人： Takeshi Yamamoto ,  Motoko Nakashizuka ,  Ichiro Terada

IPC分类号： C12N910

CPC分类号： C12N9/1081 ,  C12N9/1077

摘要： This invention provides to a novel gene encoding a protein aving the activity of &bgr;-galactoside-&agr;2,6-sialyltransferase. The gene of the invention encodes a protein which having the amino acid sequence of SEQ ID NO:1, or a protein having the amino acid sequence of SEQ ID NO:1 which have been modified by deletion, substitution or addition of at least one amino acid residue in said sequence, while maintaining substantially the same &bgr;-galactoside-&agr;2,6-sialyltransferase activity. The present invention uses said gene to further provide a vector for expressing a protein having the &bgr;-galactoside-&agr;2,6-sialyltransferase activity, host cells and a recombinant protein. The protein encoded by the gene of the invention does not have any substantial homology with known sialyltransferases, and in addition, the membrane binding region is located in the C-terminal unlike known sialyltransferases.

摘要翻译： 本发明提供了一种新型的编码β-半乳糖苷-α2,6-唾液酸转移酶活性蛋白质的基因。本发明的基因编码具有SEQ ID NO：1的氨基酸序列的蛋白质，或具有 通过在所述序列中缺失，取代或添加至少一个氨基酸残基而修饰的SEQ ID NO：1的氨基酸序列，同时保持基本上相同的β-半乳糖苷-α2,6-唾液酸转移酶活性。本发明使用 所述基因进一步提供用于表达具有β-半乳糖苷-α2,6-唾液酸转移酶活性的蛋白质的载体，宿主细胞和重组蛋白。 由本发明的基因编码的蛋白质与已知的唾液酸转移酶不具有任何实质的同源性，此外，与已知的唾液酸转移酶不同，膜结合区位于C末端。