公开(公告)号：US10717782B2

公开(公告)日：2020-07-21

申请号：US16141491

申请日：2018-09-25

Applicant: TRUSTEES OF BOSTON UNIVERSITY

Inventor： John T. Ngo

IPC: C07K16/28 ,  C07K19/00 ,  C07K14/705 ,  C07K14/71 ,  C07K16/30 ,  C07K16/46 ,  C12N5/09

Abstract: Described herein are compositions, methods, and systems for modulating Notch receptor activation. Aspects of the invention relate to synthetic proteins comprising at least a Notch NRR (Negative Regulatory Region)-binding scFV fused to a transmembrane domain. Another aspect of the invention relates to drug-dependent synthetic proteins. Constructs and engineered cells comprising the synthetic proteins are additionally described herein.

公开(公告)号：US12065501B2

公开(公告)日：2024-08-20

申请号：US17072434

申请日：2020-10-16

Applicant: TRUSTEES OF BOSTON UNIVERSITY

Inventor： John T. Ngo

IPC: C07K16/28 ,  C07K14/705 ,  C07K14/71 ,  C07K16/30 ,  C07K16/46 ,  C07K19/00 ,  C12N5/09 ,  C12N9/50

CPC classification number: C07K16/30 ,  C07K14/705 ,  C07K16/2863 ,  C07K16/462 ,  C07K19/00 ,  C12N5/0693 ,  C12N9/50 ,  C07K16/28 ,  C07K2317/56 ,  C07K2317/569 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/76 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/70 ,  C07K2319/74

Abstract: Described herein are compositions, methods, and systems for modulating Notch receptor activation. Aspects of the invention relate to synthetic proteins comprising at least a Notch NRR (Negative Regulatory Region)-binding scFV fused to a transmembrane domain. Another aspect of the invention relates to drug-dependent synthetic proteins. Constructs and engineered cells comprising the synthetic proteins are additionally described herein.

公开(公告)号：US12043652B2

公开(公告)日：2024-07-23

申请号：US17458739

申请日：2021-08-27

Applicant: TRUSTEES OF BOSTON UNIVERSITY

Inventor： Elliot Parker Tague ,  John T. Ngo ,  Jeffrey Blye McMahan

IPC: C07K14/705

CPC classification number: C07K14/705 ,  C07K2319/03

Abstract: Described herein are methods and compositions related to a modular engineered receptor polypeptide construct and their use in methods to modulate the activity of a cell. In particular, the disclosure relates to an engineered receptor polypeptide comprising, in brief, (i) an extracellular ligand binding domain having at least one ligand binding site, (ii) an optional flexible polypeptide linker, (iii) an intramolecular peptide that binds to the at least one ligand binding site in the extracellular ligand binding domain, (iv) a transmembrane domain comprising at least one γ-secretase cleavage site, and (v) an intracellular effector domain, where the intramolecular peptide that serves to regulate the activity of the engineered receptor polypeptide. Other aspects relate to cells comprising the engineered receptor polypeptide, and nucleic acid sequence encoding the engineered receptor polypeptide.

公开(公告)号：US11672828B2

公开(公告)日：2023-06-13

申请号：US17171329

申请日：2021-02-09

Applicant: TRUSTEES OF BOSTON UNIVERSITY

Inventor： John T. Ngo ,  Jeffrey Blye McMahan

IPC: A61K35/17 ,  C07K14/725 ,  C07K14/705 ,  C07K16/28 ,  C07K16/44

CPC classification number: A61K35/17 ,  C07K14/705 ,  C07K14/7051 ,  C07K14/70521 ,  C07K16/28 ,  C07K16/2809 ,  C07K16/2818 ,  C07K16/44 ,  C07K2319/03 ,  C07K2319/30 ,  C07K2319/60 ,  C07K2319/71 ,  C07K2319/74 ,  C07K2319/80

Abstract: Described herein are polypeptides, systems, and methods that relate to using domains that bind specifically to a biotinylamide to control receptor and cellular activity.

公开(公告)号：US11660288B2

公开(公告)日：2023-05-30

申请号：US17462185

申请日：2021-08-31

Applicant: TRUSTEES OF BOSTON UNIVERSITY

Inventor： John T. Ngo ,  Mary Dunlop ,  Elliot Parker Tague ,  Nathan Michael Tague ,  Alexander Michael Marzilli

IPC: A61K31/404 ,  A61K31/5377 ,  A61K31/4965 ,  C07K14/435 ,  A61K47/64 ,  C07K14/705 ,  C12N9/12 ,  C07K14/02 ,  C07K14/47

CPC classification number: A61K31/404 ,  A61K31/4965 ,  A61K31/5377 ,  A61K47/6425 ,  C07K14/02 ,  C07K14/43595 ,  C07K14/47 ,  C07K14/705 ,  C12N9/1241 ,  C07K2319/03 ,  C07K2319/60 ,  C07K2319/80

Abstract: The technology described herein is directed to polypeptide systems using drug-controlled peptide docking domains and cognate docking domain-binding peptides and their use to control cellular signaling, activity, and/or gene expression.

公开(公告)号：US10858443B2

公开(公告)日：2020-12-08

申请号：US15994330

申请日：2018-05-31

Applicant: TRUSTEES OF BOSTON UNIVERSITY

Inventor： John T. Ngo

IPC: C07K16/28 ,  C07K19/00 ,  C07K14/705 ,  C07K14/71 ,  C07K16/30 ,  C07K16/46 ,  C12N5/09 ,  C12N9/50

Abstract: Described herein are compositions, methods, and systems for modulating Notch receptor activation. Aspects of the invention relate to synthetic proteins comprising at least a Notch NRR (Negative Regulatory Region)-binding scFV fused to a transmembrane domain. Another aspect of the invention relates to drug-dependent synthetic proteins. Constructs and engineered cells comprising the synthetic proteins are additionally described herein.