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公开(公告)号：US20210024921A1
公开(公告)日：2021-01-28
申请号：US16467233
申请日：2017-12-14
申请人： The J. David Gladstone Institutes, a testamentary trust established under theWill ofJ.DavidGladstone , The Regents of the University of California
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/6869
摘要： Provided are methods and compositions for generating a deletion library, and methods and compositions for generating and identifying a defective interfering particle (DIP). Also provided are transposon cassettes. A subject method can include: inserting a transposon cassette comprising a target sequence for a sequence specific DNA endonuclease into a population of circular target DNAs to generate a population of transposon-inserted circular target DNAs; contacting the population of transposon-inserted circular target DNAs with the sequence specific DNA endonuclease to generate a population of cleaved linear target DNAs; contacting the population of cleaved linear target DNAs with one or more exonucleases to generate a population of deletion DNAs; and circularizing the deletion DNAs to generate a library of circularized deletion DNAs. The population of circular target DNAs can include viral genomic DNA. Also provided are human immunodeficiency virus (HIV) deletion mutants, e.g., interfering, conditionally replicating, HIV deletion mutants, and related constructs.
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公开(公告)号：US11584931B2
公开(公告)日：2023-02-21
申请号：US16467233
申请日：2017-12-14
申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone , The Regents of the University of California
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/6869 , C12N15/66 , C12N15/867
摘要： Provided are methods and compositions for generating a deletion library, and methods and compositions for generating and identifying a defective interfering particle (DIP). Also provided are transposon cassettes. A subject method can include: inserting a transposon cassette comprising a target sequence for a sequence specific DNA endonuclease into a population of circular target DNAs to generate a population of transposon-inserted circular target DNAs; contacting the population of transposon-inserted circular target DNAs with the sequence specific DNA endonuclease to generate a population of cleaved linear target DNAs; contacting the population of cleaved linear target DNAs with one or more exonucleases to generate a population of deletion DNAs; and circularizing the deletion DNAs to generate a library of circularized deletion DNAs. The population of circular target DNAs can include viral genomic DNA. Also provided are human immunodeficiency virus (HIV) deletion mutants, e.g., interfering, conditionally replicating, HIV deletion mutants, and related constructs.
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3.发明申请公开(公告)号：US20210395746A1
公开(公告)日：2021-12-23
申请号：US17251395
申请日：2019-06-12
申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads
IPC分类号： C12N15/113 , A61K31/713 , A61K31/522 , A61K31/662 , A61K31/675 , A61K31/7056
摘要： The present disclosure provides compositions and methods for inhibiting herpesvirus replication in a cell infected with herpesvirus. The composition includes a double stranded DNA molecule including a sequence of a cis regulatory sequence (crs) of a herpesvirus, wherein the crs is flanked on the 5′ end by a first sequence of at least 2 base pairs and on the 3′ end by a second sequence of at least 2 base pairs.
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公开(公告)号：US20220364119A1
公开(公告)日：2022-11-17
申请号：US17619063
申请日：2020-06-12
申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J.David Gladst
IPC分类号： C12N15/867 , A61K35/76 , A61K38/16
摘要： The present disclosure provides interfering, conditionally replicating human immunodeficiency virus (HIV) constructs; infectious particles comprising the constructs; and compositions comprising the constructs or the particles. The constructs, particles, and compositions are useful in methods of reducing HIV viral load in an individual, which methods are also provided.
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5.发明申请公开(公告)号：US20160015759A1
公开(公告)日：2016-01-21
申请号：US14769025
申请日：2014-03-13
CPC分类号： A61K35/76 , A61K38/162 , A61K39/12 , A61K39/21 , C12N7/00 , C12N2740/16021 , C12N2740/16032 , C12N2740/16033 , C12N2740/16062 , A61K2300/00
摘要： The present disclosure provides an interfering, conditionally replicating human immunodeficiency virus (HIV) construct; infectious particles comprising the constructs; and compositions comprising the construct or the particle. The constructs, particles, and compositions are useful in methods of reducing HIV viral load in an individual, which methods are also provided.
摘要翻译： 本公开提供了干扰，有条件地复制的人类免疫缺陷病毒（HIV）构建体; 包含构建体的感染性颗粒; 和包含构建体或颗粒的组合物。 构建体，颗粒和组合物可用于减少个体中HIV病毒载量的方法，也提供了这些方法。
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6.发明公开公开(公告)号：US20240035021A1
公开(公告)日：2024-02-01
申请号：US18171578
申请日：2023-02-20
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/6869 , C12N15/66
CPC分类号： C12N15/1082 , C12Q1/6806 , C12Q1/6869 , C12N15/66 , C12N15/1065 , C12N15/10 , C12Q2563/185 , C12N2800/107 , C12N2310/533 , C12N2800/90 , C12N2800/80 , C12N15/867
摘要： Provided are methods and compositions for generating a deletion library, and methods and compositions for generating and identifying a defective interfering particle (DIP). Also provided are transposon cassettes. A subject method can include: inserting a transposon cassette comprising a target sequence for a sequence specific DNA endonuclease into a population of circular target DNAs to generate a population of transposon-inserted circular target DNAs; contacting the population of transposon-inserted circular target DNAs with the sequence specific DNA endonuclease to generate a population of cleaved linear target DNAs; contacting the population of cleaved linear target DNAs with one or more exonucleases to generate a population of deletion DNAs; and circularizing the deletion DNAs to generate a library of circularized deletion DNAs. The population of circular target DNAs can include viral genomic DNA. Also provided ae human immunodeficiency virus (HIV) deletion mutants, e.g., interfering, conditionally replicating, HIV deletion mutants, and related constructs.
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公开(公告)号：US10478431B2
公开(公告)日：2019-11-19
申请号：US15527662
申请日：2015-11-24
发明人： Leor S. Weinberger
IPC分类号： A61K31/496 , A61K45/06 , A01N1/02 , A61K31/522 , A61K31/662 , A61K31/675 , A61K31/7056
摘要： The present disclosure provides a method for inhibiting cytomegalovirus (CMV) replication in a cell infected with CMV, the method comprising contacting the cell with a bisbenzimidazole compound. The present disclosure provides a method of treating a CMV infection in an individual, the method comprising administering to the individual an effective amount of a bisbenzimidazole compound.
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公开(公告)号：US20170296601A1
公开(公告)日：2017-10-19
申请号：US15498319
申请日：2017-04-26
CPC分类号： A61K35/76 , A61K38/162 , A61K39/12 , A61K39/21 , C12N7/00 , C12N2740/16021 , C12N2740/16032 , C12N2740/16033 , C12N2740/16062 , A61K2300/00
摘要： The present disclosure provides an interfering, conditionally replicating human immunodeficiency virus (HIV) construct; infectious particles comprising the constructs; and compositions comprising the construct or the particle. The constructs, particles, and compositions are useful in methods of reducing HIV viral load in an individual, which methods are also provided.
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公开(公告)号：US10106817B2
公开(公告)日：2018-10-23
申请号：US14767541
申请日：2014-02-13
发明人： Leor S. Weinberger
摘要： A recombinant expression vector comprising a nucleotide sequence encoding a herpesvirus transactivator, where the nucleotide sequence is operably linked to a herpesvirus control element is provided as are cell lines genetically modified to express a herpesvirus transactivator under the control of a herpesvirus control element. Also provided are methods of identifying agents that disrupt feedback regulation of a herpesvirus transcriptional control element by a herpesvirus transactivator.
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公开(公告)号：US20170360778A1
公开(公告)日：2017-12-21
申请号：US15527662
申请日：2015-11-24
发明人： Leor S. Weinberger
IPC分类号： A61K31/496 , A61K31/675 , A61K31/522 , A61K31/662 , A61K45/06 , A61K31/7056
CPC分类号： A61K31/496 , A01N1/0215 , A61K31/522 , A61K31/662 , A61K31/675 , A61K31/7056 , A61K45/06 , A61K2300/00
摘要： The present disclosure provides a method for inhibiting cytomegalovirus (CMV) replication in a cell infected with CMV, the method comprising contacting the cell with a bisbenzimidazole compound. The present disclosure provides a method of treating a CMV infection in an individual, the method comprising administering to the individual an effective amount of a bisbenzimidazole compound.
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